LOCUS       sequence-425941-        7704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human DDB1. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_DDB1:1-1140
FEATURES             Location/Qualifiers
     source          1..7704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4767..4888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4778..4797)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(5003..5036)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5046..5093
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5125..5919
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5179..5198)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5789..5808
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(6077..6665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6157..6176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6791..6839
                     /note="synthetic polyadenylation signal"
     CDS             complement(6863..7261)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      7258..7267
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7292..7649)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7306..7441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        7458..7649
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7606..7626
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tgtcgtacaa
     1261 ctacgtggta acggcccaga agcccaccgc cgtgaacggc tgcgtgaccg gacactttac
     1321 ttcggccgaa gacttaaacc tgttgattgc caaaaacacg agattagaga tctatgtggt
     1381 caccgccgag gggcttcggc ccgtcaaaga ggtgggcatg tatgggaaga ttgcggtcat
     1441 ggagcttttc aggcccaagg gggagagcaa ggacctgctg tttatcttga cagcgaagta
     1501 caatgcctgc atcctggagt ataaacagag tggcgagagc attgacatca ttacgcgagc
     1561 ccatggcaat gtccaggacc gcattggccg cccctcagag accggcatta ttggcatcat
     1621 tgaccctgag tgccggatga ttggcctgcg tctctatgat ggccttttca aggttattcc
     1681 actagatcgc gataataaag aactcaaggc cttcaacatc cgcctggagg agctgcatgt
     1741 cattgatgtc aagttcctat atggttgcca agcacctact atttgctttg tctaccagga
     1801 ccctcagggg cggcacgtaa aaacctatga ggtgtctctc cgagaaaagg aattcaataa
     1861 gggcccttgg aaacaggaaa atgtcgaagc tgaagcttcc atggtgatcg cagtcccaga
     1921 gccctttggg ggggccatca tcattggaca ggagtcaatc acctatcaca atggtgacaa
     1981 atacctggct attgcccctc ctatcatcaa gcaaagcacg attgtgtgcc acaatcgagt
     2041 ggaccctaat ggctcaagat acctgctggg agacatggaa ggccggctct tcatgctgct
     2101 tttggagaag gaggaacaga tggatggcac cgtcactctc aaggatctcc gtgtagaact
     2161 ccttggagag acctctattg ctgagtgctt gacatacctt gataatggtg ttgtgtttgt
     2221 cgggtctcgc ctgggtgact cccagcttgt gaagctcaac gttgacagta atgaacaagg
     2281 ctcctatgta gtggccatgg aaacctttac caacttagga cccattgtcg atatgtgcgt
     2341 ggtggacctg gagaggcagg ggcaggggca gctggtcact tgctctgggg ctttcaagga
     2401 aggttctttg cggatcatcc ggaatggaat tggaatccac gagcatgcca gcattgactt
     2461 accaggcatc aaaggattat ggccactgcg gtctgaccct aatcgtgaga ctgatgacac
     2521 tttggtgctc tcttttgtgg gccagacaag agttctcatg ttaaatggag aggaggtaga
     2581 agaaaccgaa ctgatgggtt tcgtggatga tcagcagact ttcttctgtg gcaacgtggc
     2641 tcatcagcag cttatccaga tcacttcagc atcggtgagg ttggtctctc aagaacccaa
     2701 agctctggtc agtgaatgga aggagcctca ggccaagaac atcagtgtgg cctcctgcaa
     2761 tagcagccag gtggtggtgg ctgtaggcag ggccctctac tatctgcaga tccatcctca
     2821 ggagctccgg cagatcagcc acacagagat ggaacatgaa gtggcttgct tggacatcac
     2881 cccattagga gacagcaatg gactgtcccc tctttgtgcc attggcctct ggacggacat
     2941 ctcggctcgt atcttgaagt tgccctcttt tgaactactg cacaaggaga tgctgggtgg
     3001 agagatcatt cctcgctcca tcctgatgac cacctttgag agtagccatt acctcctttg
     3061 tgccttggga gatggagcgc ttttctactt tgggctcaac attgagacag gtctgttgag
     3121 cgaccgtaag aaggtgactt tgggcaccca gcccaccgta ttgaggactt ttcgttctct
     3181 ttctaccacc aacgtctttg cttgttctga ccgccccact gtcatctata gcagcaacca
     3241 caaattggtc ttctcaaatg tcaacctcaa ggaagtgaac tacatgtgtc ccctcaattc
     3301 agatggctat cctgacagcc tggcgctggc caacaatagc accctcacca ttggcaccat
     3361 cgatgagatc cagaagctgc acattcgcac agttcccctc tatgagtctc caaggaagat
     3421 ctgctaccag gaagtgtccc agtgtttcgg ggtcctctcc agccgcattg aagtccaaga
     3481 cacgagtggg ggcacgacag ccttgaggcc cagcgctagc acccaggctc tgtccagcag
     3541 tgtaagctcc agcaagctgt tctccagcag cactgctcct catgagacct cctttggaga
     3601 agaggtggag gtgcacaacc tacttatcat tgaccaacac acctttgaag tgcttcatgc
     3661 ccaccagttt ctgcagaatg aatatgccct cagtctggtt tcctgcaagc tgggcaaaga
     3721 ccccaacact tacttcattg tgggcacagc aatggtgtat cctgaagagg cagagcccaa
     3781 gcagggtcgc attgtggtct ttcagtattc ggatggaaaa ctacagactg tggctgaaaa
     3841 ggaagtgaaa ggggccgtgt actctatggt ggaatttaac gggaagctgt tagccagcat
     3901 caatagcacg gtgcggctct atgagtggac aacagagaag gagctgcgca ctgagtgcaa
     3961 ccactacaac aacatcatgg ccctctacct gaagaccaag ggcgacttca tcctggtggg
     4021 cgaccttatg cgctcagtgc tgctgcttgc ctacaagccc atggaaggaa actttgaaga
     4081 gattgctcga gactttaatc ccaactggat gagtgctgtg gaaatcttgg atgatgacaa
     4141 ttttctgggg gctgaaaatg cctttaactt gtttgtgtgt caaaaggata gcgctgccac
     4201 cactgacgag gagcggcagc acctccagga ggttggtctt ttccacctgg gcgagtttgt
     4261 caatgtcttt tgccacggct ctctggtaat gcagaatctg ggtgagactt ccacccccac
     4321 acaaggctcg gtgctcttcg gcacggtcaa cggcatgata gggctggtga cctcactgtc
     4381 agagagctgg tacaacctcc tgctggacat gcagaatcga ctcaataaag tcatcaaaag
     4441 tgtggggaag atcgagcact ccttctggag atcctttcac accgagcgga agacagaacc
     4501 agccacaggt ttcatcgacg gtgacttgat tgagagtttc ctggatatta gccgccccaa
     4561 gatgcaggag gtggtggcaa acctacagta tgacgatggc agcggtatga agcgagaggc
     4621 cactgcagac gacctcatca aggttgtgga ggagctaact cggatccatt aatctagagg
     4681 tcaagcttcg ggggtttaaa cctgcaggca tgcaagctga tttggctgct gccaccgctg
     4741 agcaaggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     4801 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     4861 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     4921 ggttcagggg gagatgtggg aggttttttt aagcaagtaa aacctctaca aatgtggtaa
     4981 aatcttaatt aagacttcta atataacttc gtatagcata cattatacga agttatggtt
     5041 ccactgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     5101 ggaactaaac ccaggaggca gatcatgatt gaacaagatg gattgcacgc aggttctccg
     5161 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5221 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5281 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     5341 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     5401 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5461 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5521 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     5581 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     5641 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     5701 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     5761 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     5821 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     5881 cgcatcgcct tctatcgcct tcttgacgag ttcttctgat tcgaaatgac cgaccaagcg
     5941 acgcccaacc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6001 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6061 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6121 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6181 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6241 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6301 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6361 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6421 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6481 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     6541 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6601 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6661 ttcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6721 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttatagt
     6781 ccggaatcgt cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg
     6841 cggccatcgt gatggctaga gtttagccct cccacacata accagagggc agcaattcac
     6901 gaatcccaac tgccgtcggc tgtccatcac tgtccttcac tatggctttg atcccaggat
     6961 gcagatcgag aagcacctgt cggcaccgtc cgcaggggct caagatgccc ctgttctcat
     7021 ttccgatcgc gacgatacaa gtcaggttgc cagctgccgc agcagcagca gtgcccagca
     7081 ccacgagttc tgcacaaggt cccccagtaa aatgatatac attgacacca gtgaagatgc
     7141 ggccgtcgct agagagagct gcgctggcga cgctgtagtc ttcagagatg gggatgctgt
     7201 tgattgtagc cgttgctctt tcaatgaggg tggattcttc ttgagacaaa ggcttggcca
     7261 tggtggcgct agtgtcagaa gaatcgagct ttttgcaaaa gcctaggcct ccaaaaaagc
     7321 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     7381 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     7441 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
     7501 ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
     7561 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
     7621 cattccacag ctggttcttt ccgcctcaga aggtacctaa ccaagttcct ctttcagagg
     7681 ttatttcagg ccatggtgct gcgc
//
