LOCUS       sequence-425949-        6114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR46. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_WDR46:1-610
FEATURES             Location/Qualifiers
     source          1..6114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3177..3298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3188..3207)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3413..3446)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3456..3503
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3535..4329
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3589..3608)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4199..4218
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4487..5075)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4567..4586)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5201..5249
                     /note="synthetic polyadenylation signal"
     CDS             complement(5273..5671)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5668..5677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5702..6059)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5716..5851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5868..6059
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6016..6036
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tggagacagc
     1261 ccccaagccg ggcaaggatg tcccgcccaa gaaagacaaa cttcagacca agagaaagaa
     1321 accgcggcga tactgggagg aagagaccgt tccgaccaca gccggagcct ctccagggcc
     1381 tcctcgtaac aagaagaatc gggagctccg tcctcagaga ccaaaaaatg cttacatctt
     1441 aaagaagtct cggatctcta agaagcctca ggtcccgaag aaaccccgag aatggaagaa
     1501 cccggagtcc cagcgcggct tgtccggggc ccaagatcca ttcccaggcc ccgcccccgt
     1561 ccctgtggaa gtggtccaga agttctgtcg cattgacaaa tcccgaaagc taccacattc
     1621 taaagccaaa actcgaagcc gacttgaggt ggctgaagct gaggaagagg aaacaagtat
     1681 caaagctgct cgttctgagc tgctgcttgc tgaagaacct gggtttctgg aaggggagga
     1741 tggggaagac acagcaaaga tatgccaggc tgacattgtg gaggctgtgg acattgcaag
     1801 tgcagccaag cactttgact tgaatctgcg gcagtttgga ccctacagac taaactactc
     1861 tcgaactgga agacacctgg cttttggagg gcgccgaggt catgtggctg cccttgattg
     1921 ggtaacaaag aagcttatgt gcgagatcaa cgtcatggag gcggtgcggg acatccggtt
     1981 tctccattct gaggcactgc ttgctgttgc tcagaaccgc tggctccaca tctatgacaa
     2041 tcagggcatt gagctccact gtatccgccg ctgtgaccga gtaacacggc ttgagttcct
     2101 gcccttccac ttcctcctgg ctacagcttc agaaacaggg tttctaacct acctggatgt
     2161 gtcagtgggg aagattgtgg cagctctgaa tgctcgagct gggcggctcg atgttatgag
     2221 tcagaaccct tacaatgccg tcatccatct cggacacagc aatggtactg tgtctttatg
     2281 gagtccagct atgaaggagc cactggcaaa gattctctgt catcgtggtg gggtccgggc
     2341 tgtggcagta gattctacag gcacgtacat ggccacctct ggcctagacc accagctgaa
     2401 gatctttgac ttgcgaggga cgtaccagcc tctgagcact cggaccctgc cccatggagc
     2461 agggcacctg gccttctccc agaggggact gctggtggcg ggaatgggtg acgttgtcaa
     2521 catctgggca gggcagggca aggccagccc accctccctt gaacagccct acctcaccca
     2581 ccggctctca ggccctgtgc atggccttca gttctgcccc tttgaagatg tgctgggggt
     2641 ggggcacact gggggcatca ccagcatgct ggtccctggg gccggtgagc ccaacttcga
     2701 tggcctggag agtaatccat acagaagccg gaagcagcgc caggagtggg aggtgaaggc
     2761 cctgctagag aaggtacctg cagagcttat ttgtctggac ccacgagccc tggccgaggt
     2821 ggatgtcatc tccctggagc agggaaagaa ggagcagata gagaggctgg gctatgaccc
     2881 gcaggctaag gctcccttcc agccaaagcc aaagcagaag ggccgcagct ccacggcaag
     2941 cctggtgaag aggaagagga aggtcatgga tgaggaacac agggacaagg tccggcagag
     3001 ccttcagcag cagcatcata aggaggcgaa ggccaagccc acgggggccc ggccatctgc
     3061 cctggacaga tttgtgcgct aatctagagg tcaagcttcg ggggtttaaa cctgcaggca
     3121 tgcaagctga tttggctgct gccaccgctg agcaaggccg cttcgagcag acatgataag
     3181 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     3241 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3301 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggttttttt
     3361 aagcaagtaa aacctctaca aatgtggtaa aatcttaatt aagacttcta atataacttc
     3421 gtatagcata cattatacga agttatggtt ccactgttga caattaatca tcggcatagt
     3481 atatcggcat agtataatac gacaaggtga ggaactaaac ccaggaggca gatcatgatt
     3541 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     3601 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     3661 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     3721 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     3781 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     3841 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     3901 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     3961 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4021 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     4081 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4141 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     4201 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     4261 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     4321 ttcttctgat tcgaaatgac cgaccaagcg acgcccaacc ggtatcagct cactcaaagg
     4381 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4441 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4501 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4561 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4621 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4681 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4741 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4801 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4861 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4921 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4981 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5041 agcagcagat tacgcgcaga aaaaaaggat ttcaagaaga tcctttgatc ttttctacgg
     5101 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5161 aaaggatctt cacctagatc cttttatagt ccggaatcgt cacacaaaaa accaacacac
     5221 agatgtaatg aaaataaaga tattttattg cggccatcgt gatggctaga gtttagccct
     5281 cccacacata accagagggc agcaattcac gaatcccaac tgccgtcggc tgtccatcac
     5341 tgtccttcac tatggctttg atcccaggat gcagatcgag aagcacctgt cggcaccgtc
     5401 cgcaggggct caagatgccc ctgttctcat ttccgatcgc gacgatacaa gtcaggttgc
     5461 cagctgccgc agcagcagca gtgcccagca ccacgagttc tgcacaaggt cccccagtaa
     5521 aatgatatac attgacacca gtgaagatgc ggccgtcgct agagagagct gcgctggcga
     5581 cgctgtagtc ttcagagatg gggatgctgt tgattgtagc cgttgctctt tcaatgaggg
     5641 tggattcttc ttgagacaaa ggcttggcca tggtggcgct agtgtcagaa gaatcgagct
     5701 ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     5761 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     5821 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     5881 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     5941 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     6001 ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcaga
     6061 aggtacctaa ccaagttcct ctttcagagg ttatttcagg ccatggtgct gcgc
//
