LOCUS       sequence-425951-        6822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human DDB1. loop deletion amino 
            acids I396-D689. N-terminal HiBiT tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_DDB1:1-1140
FEATURES             Location/Qualifiers
     source          1..6822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3885..4006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3896..3915)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(4121..4154)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4164..4211
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4243..5037
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4297..4316)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4907..4926
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(5195..5783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5275..5294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5909..5957
                     /note="synthetic polyadenylation signal"
     CDS             complement(5981..6379)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6376..6385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6410..6767)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6424..6559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6576..6767
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6724..6744
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tgtcgtacaa
     1261 ctacgtggta acggcccaga agcccaccgc cgtgaacggc tgcgtgaccg gacactttac
     1321 ttcggccgaa gacttaaacc tgttgattgc caaaaacacg agattagaga tctatgtggt
     1381 caccgccgag gggcttcggc ccgtcaaaga ggtgggcatg tatgggaaga ttgcggtcat
     1441 ggagcttttc aggcccaagg gggagagcaa ggacctgctg tttatcttga cagcgaagta
     1501 caatgcctgc atcctggagt ataaacagag tggcgagagc attgacatca ttacgcgagc
     1561 ccatggcaat gtccaggacc gcattggccg cccctcagag accggcatta ttggcatcat
     1621 tgaccctgag tgccggatga ttggcctgcg tctctatgat ggccttttca aggttattcc
     1681 actagatcgc gataataaag aactcaaggc cttcaacatc cgcctggagg agctgcatgt
     1741 cattgatgtc aagttcctat atggttgcca agcacctact atttgctttg tctaccagga
     1801 ccctcagggg cggcacgtaa aaacctatga ggtgtctctc cgagaaaagg aattcaataa
     1861 gggcccttgg aaacaggaaa atgtcgaagc tgaagcttcc atggtgatcg cagtcccaga
     1921 gccctttggg ggggccatca tcattggaca ggagtcaatc acctatcaca atggtgacaa
     1981 atacctggct attgcccctc ctatcatcaa gcaaagcacg attgtgtgcc acaatcgagt
     2041 ggaccctaat ggctcaagat acctgctggg agacatggaa ggccggctct tcatgctgct
     2101 tttggagaag gaggaacaga tggatggcac cgtcactctc aaggatctcc gtgtagaact
     2161 ccttggagag acctctattg ctgagtgctt gacatacctt gataatggtg ttgtgtttgt
     2221 cgggtctcgc ctgggtgact cccagcttgt gaagctcaac gttgacagta atgaacaagg
     2281 ctcctatgta gtggccatgg aaacctttac caacttagga cccattgtcg atatgtgcgt
     2341 ggtggacctg gagaggcagg ggcaggggca gctggtcact tgctctgggg ctttcaagga
     2401 aggttctttg cggatcatcc ggaatggaat tggaagcctg gcgctggcca acaatagcac
     2461 cctcaccatt ggcaccatcg atgagatcca gaagctgcac attcgcacag ttcccctcta
     2521 tgagtctcca aggaagatct gctaccagga agtgtcccag tgtttcgggg tcctctccag
     2581 ccgcattgaa gtccaagaca cgagtggggg cacgacagcc ttgaggccca gcgctagcac
     2641 ccaggctctg tccagcagtg taagctccag caagctgttc tccagcagca ctgctcctca
     2701 tgagacctcc tttggagaag aggtggaggt gcacaaccta cttatcattg accaacacac
     2761 ctttgaagtg cttcatgccc accagtttct gcagaatgaa tatgccctca gtctggtttc
     2821 ctgcaagctg ggcaaagacc ccaacactta cttcattgtg ggcacagcaa tggtgtatcc
     2881 tgaagaggca gagcccaagc agggtcgcat tgtggtcttt cagtattcgg atggaaaact
     2941 acagactgtg gctgaaaagg aagtgaaagg ggccgtgtac tctatggtgg aatttaacgg
     3001 gaagctgtta gccagcatca atagcacggt gcggctctat gagtggacaa cagagaagga
     3061 gctgcgcact gagtgcaacc actacaacaa catcatggcc ctctacctga agaccaaggg
     3121 cgacttcatc ctggtgggcg accttatgcg ctcagtgctg ctgcttgcct acaagcccat
     3181 ggaaggaaac tttgaagaga ttgctcgaga ctttaatccc aactggatga gtgctgtgga
     3241 aatcttggat gatgacaatt ttctgggggc tgaaaatgcc tttaacttgt ttgtgtgtca
     3301 aaaggatagc gctgccacca ctgacgagga gcggcagcac ctccaggagg ttggtctttt
     3361 ccacctgggc gagtttgtca atgtcttttg ccacggctct ctggtaatgc agaatctggg
     3421 tgagacttcc acccccacac aaggctcggt gctcttcggc acggtcaacg gcatgatagg
     3481 gctggtgacc tcactgtcag agagctggta caacctcctg ctggacatgc agaatcgact
     3541 caataaagtc atcaaaagtg tggggaagat cgagcactcc ttctggagat cctttcacac
     3601 cgagcggaag acagaaccag ccacaggttt catcgacggt gacttgattg agagtttcct
     3661 ggatattagc cgccccaaga tgcaggaggt ggtggcaaac ctacagtatg acgatggcag
     3721 cggtatgaag cgagaggcca ctgcagacga cctcatcaag gttgtggagg agctaactcg
     3781 gatccattaa tctagaggtc aagcttcggg ggtttaaacc tgcaggcatg caagctgatt
     3841 tggctgctgc caccgctgag caaggccgct tcgagcagac atgataagat acattgatga
     3901 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     3961 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     4021 cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa
     4081 cctctacaaa tgtggtaaaa tcttaattaa gacttctaat ataacttcgt atagcataca
     4141 ttatacgaag ttatggttcc actgttgaca attaatcatc ggcatagtat atcggcatag
     4201 tataatacga caaggtgagg aactaaaccc aggaggcaga tcatgattga acaagatgga
     4261 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     4321 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     4381 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     4441 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     4501 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     4561 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     4621 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     4681 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     4741 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     4801 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     4861 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     4921 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     4981 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgattc
     5041 gaaatgaccg accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt
     5101 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5161 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5221 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5281 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5341 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5401 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5461 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5521 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5581 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     5641 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5701 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5761 cgcgcagaaa aaaaggattt caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5821 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5881 cctagatcct tttatagtcc ggaatcgtca cacaaaaaac caacacacag atgtaatgaa
     5941 aataaagata ttttattgcg gccatcgtga tggctagagt ttagccctcc cacacataac
     6001 cagagggcag caattcacga atcccaactg ccgtcggctg tccatcactg tccttcacta
     6061 tggctttgat cccaggatgc agatcgagaa gcacctgtcg gcaccgtccg caggggctca
     6121 agatgcccct gttctcattt ccgatcgcga cgatacaagt caggttgcca gctgccgcag
     6181 cagcagcagt gcccagcacc acgagttctg cacaaggtcc cccagtaaaa tgatatacat
     6241 tgacaccagt gaagatgcgg ccgtcgctag agagagctgc gctggcgacg ctgtagtctt
     6301 cagagatggg gatgctgttg attgtagccg ttgctctttc aatgagggtg gattcttctt
     6361 gagacaaagg cttggccatg gtggcgctag tgtcagaaga atcgagcttt ttgcaaaagc
     6421 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     6481 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     6541 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     6601 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg
     6661 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     6721 cacaccctaa ctgacacaca ttccacagct ggttctttcc gcctcagaag gtacctaacc
     6781 aagttcctct ttcagaggtt atttcaggcc atggtgctgc gc
//
