LOCUS       sequence-425966-        9171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human XPO1. C-terminal NanoLuc 
            luciferase tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pNLF1-C_XPO1:1-1071
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4346..4858
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(4934..4952)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4989..5110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5000..5019)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5054..5073
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5355..5712
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5355..5546
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5378..5398)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5563..5698
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5743..6780
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             6759..6767
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    6804..6852
                     /note="synthetic polyadenylation signal"
     promoter        7008..7112
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7113..7181,7182..7973)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7331..7350)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8131..8719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8211..8230)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8835..9118
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gcgatcgctt ccgaattcgc
     1081 caccatgcca gcaattatga caatgttagc agaccatgca gctcgtcagc tgcttgattt
     1141 cagccaaaaa ctggatatca acttattaga taatgtggtg aattgcttat accatggaga
     1201 aggagcccag caaagaatgg ctcaagaagt actgacacat ttaaaggagc atcctgatgc
     1261 ttggacaaga gtcgacacaa ttttggaatt ttctcagaat atgaatacga aatactatgg
     1321 actacaaatt ttggaaaatg tgataaaaac aaggtggaag attcttccaa ggaaccagtg
     1381 cgaaggaata aaaaaatacg ttgttggcct cattatcaag acgtcatctg acccaacttg
     1441 tgtagagaaa gaaaaggtgt atatcggaaa attaaatatg atccttgttc agatactgaa
     1501 acaagaatgg cccaaacatt ggccaacttt tatcagtgat attgttggag caagtaggac
     1561 cagcgaaagt ctctgtcaaa ataatatggt gattcttaaa ctcttgagtg aagaagtatt
     1621 tgatttctct agtggacaga taacccaagt caaatctaag catttaaaag acagcatgtg
     1681 caatgaattc tcacagatat ttcaactgtg tcagtttgta atggaaaatt ctcaaaatgc
     1741 tccacttgta catgcaacct tggaaacatt gctcagattt ctgaactgga ttcccctggg
     1801 atatattttt gagaccaaat taatcagcac attgatttat aagttcctga atgttccaat
     1861 gtttcgaaat gtctctctga agtgcctcac tgagattgct ggtgtgagtg taagccaata
     1921 tgaagaacaa tttgtaacac tatttactct gacaatgatg caactaaagc agatgcttcc
     1981 tttaaatacc aatattcgac ttgcgtactc aaatggaaaa gatgatgaac agaacttcat
     2041 tcaaaatctc agtttgtttc tctgcacctt tcttaaggaa catgatcaac ttatagaaaa
     2101 aagattaaat ctcagggaaa ctcttatgga ggcccttcat tatatgttgt tggtatctga
     2161 agtagaagaa actgaaatct ttaaaatttg tcttgaatac tggaatcatt tggctgctga
     2221 actctataga gagagtccat tctctacatc tgcctctccg ttgctttctg gaagtcaaca
     2281 ttttgatgtt cctcccagga gacagctata tttgcccatg ttattcaagg tccgtttatt
     2341 aatggttagt cgaatggcta aaccagagga agtattggtt gtagagaatg atcaaggaga
     2401 agttgtgaga gaattcatga aggatacaga ttccataaat ttgtataaga atatgaggga
     2461 aacattggtt tatcttactc atctggatta tgtagataca gaaagaataa tgacagagaa
     2521 gcttcacaat caagtgaatg gtacagagtg gtcatggaaa aatttgaata cattgtgttg
     2581 ggcaataggc tccattagtg gagcaatgca tgaagaggac gaaaaacgat ttcttgttac
     2641 tgttataaag gatctattag gattatgtga acagaaaaga ggcaaagata ataaagctat
     2701 tattgcatca aatatcatgt acatagtagg tcaataccca cgttttttga gagctcactg
     2761 gaaatttctg aagactgtag ttaacaagct gttcgaattc atgcatgaga cccatgatgg
     2821 agtccaggat atggcttgtg atactttcat taaaatagcc caaaaatgcc gcaggcattt
     2881 cgttcaggtt caggttggag aagtgatgcc atttattgat gaaattttga acaacattaa
     2941 cactattatt tgtgatcttc agcctcaaca ggttcatacg ttttatgaag ctgtggggta
     3001 catgattggt gcacaaacag atcaaacagt acaagaacac ttgatagaaa agtacatgtt
     3061 actccctaat caagtgtggg atagtataat ccagcaggca accaaaaatg tggatatact
     3121 gaaagatcct gaaacagtca agcagcttgg tagcattttg aaaacaaatg tgagagcctg
     3181 caaagctgtt ggacacccct ttgtaattca gcttggaaga atttatttag atatgcttaa
     3241 tgtatacaag tgcctcagtg aaaatatttc tgcagctatc caagctaatg gtgaaatggt
     3301 tacaaagcaa ccattgatta gaagtatgcg aactgtaaaa agggaaactt taaagttaat
     3361 atctggttgg gtgagccgat ccaatgatcc acagatggtc gctgaaaatt ttgttccccc
     3421 tctgttggat gcagttctca ttgattatca gagaaatgtc ccagctgcta gagaaccaga
     3481 agtgcttagt actatggcca taattgtcaa caagttaggg ggacatataa cagctgaaat
     3541 acctcaaata tttgatgctg tttttgaatg cacattgaat atgataaata aggactttga
     3601 agaatatcct gaacatagaa cgaacttttt cttactactt caggctgtca attctcattg
     3661 tttcccagca ttccttgcta ttccacctac acagtttaaa cttgttttgg attccatcat
     3721 ttgggctttc aaacatacta tgaggaatgt cgcagatacg ggcttacaga tactttttac
     3781 actcttacaa aatgttgcac aagaagaagc tgcagctcag agtttttatc aaacttattt
     3841 ttgtgatatt ctccagcata tcttttctgt tgtgacagac acttcacata ctgctggttt
     3901 aacaatgcat gcatcaattc ttgcatatat gtttaatttg gttgaagaag gaaaaataag
     3961 tacatcatta aatcctggaa atccagttaa caaccaaatc tttcttcagg aatatgtggc
     4021 taatctcctt aagtcggcct tccctcacct acaagatgct caagtaaagc tctttgtgac
     4081 agggcttttc agcttaaatc aagatattcc tgctttcaag gaacatttaa gagatttcct
     4141 agttcaaata aaggaatttg caggtgaaga cacttctgat ttgtttttgg aagagagaga
     4201 aatagcccta cggcaggctg atgaagagaa acataaacgt caaatgtctg tccctggcat
     4261 ctttaatcca catgagattc cagaagaaat gtgtgattct agatttgggc ccaattcctg
     4321 caggcgagct ctcggctcga gcggcgtctt cacactcgaa gatttcgttg gggactggcg
     4381 acagacagcc ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt
     4441 tcagaatctc ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg
     4501 gctgaagatc gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg
     4561 ccagatcgaa aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat
     4621 cctgcactat ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg
     4681 acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct
     4741 gtggaacggc aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt
     4801 ccgagtaacc atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcgtaagg
     4861 ccgcgactct agggcatgca agctgatccg gctgctaaca aagcccgaaa ggaagctgag
     4921 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcggc cgcttcgagc
     4981 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     5041 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     5101 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     5161 ggaggttttt ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa
     5221 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5281 acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
     5341 tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     5401 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5461 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5521 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     5581 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5641 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5701 ggcttttgca aaaagctcga ttcttctgac actagcgcca ccatgaagaa gcccgaactc
     5761 accgctacca gcgttgaaaa atttctcatc gagaagttcg acagtgtgag cgacctgatg
     5821 cagttgtcgg agggcgaaga gagccgagcc ttcagcttcg atgtcggcgg acgcggctat
     5881 gtactgcggg tgaatagctg cgctgatggc ttctacaaag accgctacgt gtaccgccac
     5941 ttcgccagcg ctgcactacc catccccgaa gtgttggaca tcggcgagtt cagcgagagc
     6001 ctgacatact gcatcagtag acgcgcccaa ggcgttactc tccaagacct ccccgaaaca
     6061 gagctgcctg ctgtgttaca gcctgtcgcc gaagctatgg atgctattgc cgccgccgac
     6121 ctcagtcaaa ccagcggctt cggcccattc gggccccaag gcatcggcca gtacacaacc
     6181 tggcgggatt tcatttgcgc cattgctgat ccccatgtct accactggca gaccgtgatg
     6241 gacgacaccg tgtccgccag cgtagctcaa gccctggacg aactgatgct gtgggccgaa
     6301 gactgtcccg aggtgcgcca cctcgtccat gccgacttcg gcagcaacaa cgtcctgacc
     6361 gacaacggcc gcatcaccgc cgtaatcgac tggtccgaag ctatgttcgg ggacagtcag
     6421 tacgaggtgg ccaacatctt cttctggcgg ccctggctgg cttgcatgga gcagcagact
     6481 cgctacttcg agcgccggca tcccgagctg gccggcagcc ctcgtctgcg agcctacatg
     6541 ctgcgcatcg gcctggatca gctctaccag agcctcgtgg acggcaactt cgacgatgct
     6601 gcctgggctc aaggccgctg cgatgccatc gtccgcagcg gggccggcac cgtcggtcgc
     6661 acacaaatcg ctcgccggag cgcagccgta tggaccgacg gctgcgtcga ggtgctggcc
     6721 gacagcggca accgccggcc cagtacacga ccgcgcgcta aggaggtagg tcgagtttag
     6781 actctagcca tcacgatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     6841 gttttttgtg tgaatcgata gcgataagga tcctctttgc gcttgcgttt tcccttgtcc
     6901 agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct
     6961 acgtaatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7021 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7081 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7141 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7201 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7261 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7321 tcaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7381 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7441 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7501 acactgcggc caacttactt ctgacaacta tcggaggacc gaaggagcta accgcttttt
     7561 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7621 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7681 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7741 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7801 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7861 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7921 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taattcgaaa
     7981 tgaccgacca agcgacgccc aaccggtatc agctcactca aaggcggtaa tacggttatc
     8041 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8101 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8161 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8221 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8281 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8341 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8401 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8461 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8521 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8581 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8641 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8701 cagaaaaaaa ggatttcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8761 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8821 gatcctttta tagtccggaa atacaggaac gcacgctgga tggcccttcg ctgggatggt
     8881 gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc ctgtaccctc
     8941 tggttgcata ggtattcata cggttaaaat ttatcaggcg cgattgcggc agtttttcgg
     9001 gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg
     9061 tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta gccatcgaga
     9121 taaaccgcag tactccggcc acgatgcgtc cggcgtagag gatcgagatc t
//
