LOCUS       sequence-425967-        9831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human PLCG1 isoform 2. 
            C-terminal NanoLuc luciferase tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 9831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pNLF1-C_PLCG1:1-1291
FEATURES             Location/Qualifiers
     source          1..9831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1079..1088
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5006..5518
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(5594..5612)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    5649..5770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5660..5679)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5714..5733
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6015..6372
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6015..6206
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6038..6058)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6223..6358
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6403..7440
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             7419..7427
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    7464..7512
                     /note="synthetic polyadenylation signal"
     promoter        7668..7772
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7773..7841,7842..8633)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7991..8010)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8791..9379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8871..8890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9495..9778
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gcgatcgctt ccgaattcgc
     1081 caccatggcg ggcgccgcgt ccccttgcgc caacggctgc gggcccggcg cgccctcgga
     1141 cgccgaggtg ctgcacctct gccgcagcct cgaggtgggc accgtcatga ctttgttcta
     1201 ctccaagaag tcgcagcgac ccgagcggaa gaccttccag gtcaagctgg agacgcgcca
     1261 gatcacgtgg agccggggcg ccgacaagat cgagggggcc attgacattc gtgaaattaa
     1321 ggagatccgc ccagggaaga cctcacggga ctttgatcgc tatcaagagg acccagcttt
     1381 ccggccggac cagtcacatt gctttgtcat tctctatgga atggaatttc gcctgaaaac
     1441 gctgagcctg caagccacat ctgaggatga agtgaacatg tggatcaagg gcttaacttg
     1501 gctgatggag gatacattgc aggcacccac acccctgcag attgagaggt ggctccggaa
     1561 gcagttttac tcagtggatc ggaatcgtga ggatcgtata tcagccaagg acctgaagaa
     1621 catgctgtcc caggtcaact accgggtccc caacatgcgc ttcctccgag agcggctgac
     1681 ggacctggag cagcgcagcg gggacatcac ctacgggcag tttgctcagc tgtaccgcag
     1741 cctcatgtac agcgcccaga agacgatgga cctccccttc ttggaagcca gtactctgag
     1801 ggctggggag cggccggagc tttgccgagt gtcccttcct gagttccagc agttccttct
     1861 tgactaccag ggggagctgt gggctgttga tcgcctccag gtgcaggagt tcatgctcag
     1921 cttcctccga gaccccttac gagagatcga ggagccatac ttcttcctgg atgagtttgt
     1981 caccttcctg ttctccaaag agaacagtgt gtggaactcg cagctggatg cagtatgccc
     2041 ggacaccatg aacaaccctc tttcccacta ctggatctcc tcctcgcaca acacgtacct
     2101 gaccggggac cagttctcca gtgagtcctc cttggaagcc tatgctcgct gcctgcggat
     2161 gggctgtcgc tgcattgagt tggactgctg ggacggcccg gatgggatgc cagttattta
     2221 ccatgggcac acccttacca ccaagatcaa gttctcagat gtcctgcaca ccatcaagga
     2281 gcatgccttt gtggcctcag agtacccagt catcctgtcc attgaggacc actgcagcat
     2341 tgcccagcag agaaacatgg cccaatactt caagaaggtg ctgggggaca cactcctcac
     2401 caagcccgtg gagatctctg ccgacgggct cccctcaccc aaccagctta agaggaagat
     2461 cctcatcaag cacaagaagc tggctgaggg cagtgcctac gaggaggtgc ctacatccat
     2521 gatgtactct gagaacgaca tcagcaactc tatcaagaat ggcatcctct acctggagga
     2581 ccctgtgaac cacgaatggt atccccacta ctttgttctg accagcagca agatctacta
     2641 ctctgaggag accagcagtg accagggcaa cgaggatgag gaggagccca aggaggtcag
     2701 cagcagcaca gagctgcact ccaatgagaa gtggttccat gggaagctag gggcagggcg
     2761 tgacgggcgt cacatcgctg agcgcctgct tactgagtac tgcatcgaga ccggagcccc
     2821 tgacggctcc ttcctcgtgc gagagagtga gaccttcgtg ggcgactaca cgctctcttt
     2881 ctggcggaac gggaaagtcc agcactgccg tatccactcc cggcaagatg ctgggacccc
     2941 caagttcttc ttgacagaca acctcgtctt tgactccctc tatgacctca tcacgcacta
     3001 ccagcaggtg cccctgcgct gtaatgagtt tgagatgcga ctttcagagc ctgtcccaca
     3061 gaccaacgcc cacgagagca aagagtggta ccacgcgagc ctgaccagag cacaggctga
     3121 gcacatgcta atgcgcgtcc ctcgtgatgg ggccttcctg gtgcggaagc ggaatgaacc
     3181 caactcatat gccatctctt tccgggctga gggcaagatc aagcattgcc gtgtccagca
     3241 agagggccag acagtgatgc tagggaactc ggagttcgac agccttgttg acctcatcag
     3301 ctactatgag aaacacccgc tataccgcaa gatgaagctg cgctatccca tcaacgagga
     3361 ggcactggag aagattggca cagctgagcc tgactacggg gccctgtatg agggacgcaa
     3421 ccctggcttc tatgtagagg caaaccctat gccaactttc aagtgtgcag tcaaagccct
     3481 ctttgactac aaggcccaga gggaggacga gctgaccttc accaagagcg ccatcatcca
     3541 gaatgtggag aagcaagagg gaggctggtg gcgaggggac tacggaggga agaagcagct
     3601 gtggttccca tcaaactacg tggaagagat ggtcaacccc gtggccctgg agccggagag
     3661 ggagcacttg gacgagaaca gccccctagg ggacttgctg cggggggtct tggatgtgcc
     3721 ggcttgtcag attgccatcc gtcctgaggg caagaacaac cggctcttcg tcttctccat
     3781 cagcatggcg tcggtggccc actggtccct ggatgttgct gccgactcac aggaggagct
     3841 gcaggactgg gtgaaaaaga tccgtgaagt ggcccagaca gcagacgcca ggctcactga
     3901 agggaagata atggaacgga ggaagaagat tgccctggag ctctctgaac ttgtcgtcta
     3961 ctgccggcct gttccctttg atgaagagaa gattggcaca gaacgtgctt gctaccggga
     4021 catgtcatcc ttcccggaaa ccaaggctga gaaatacgtg aacaaggcca aaggcaagaa
     4081 gttccttcag tacaatcgac tgcagctctc ccgcatctac cccaagggcc agcgactgga
     4141 ttcctccaac tacgatcctt tgcccatgtg gatctgtggc agtcagcttg tggccctcaa
     4201 cttccagacc cctgacaagc ctatgcagat gaaccaggcc ctcttcatga cgggcaggca
     4261 ctgtggctac gtgctgcagc caagcaccat gcgggatgag gccttcgacc cctttgacaa
     4321 gagcagcctc cgcgggctgg agccatgtgc catctctatt gaggtgctgg gggcccgaca
     4381 tctgccaaag aatggccgag gcattgtgtg tccttttgtg gagattgagg tggctggagc
     4441 tgagtatgac agcaccaagc agaagacaga gtttgtggtg gacaatggac tcaaccctgt
     4501 atggccagcc aagcccttcc acttccagat cagtaaccct gaatttgcct ttctgcgctt
     4561 cgtggtgtat gaggaagaca tgtttagtga ccagaatttc ctggctcagg ctactttccc
     4621 agtaaaaggc ctgaagacag gatacagagc agtgcctttg aagaacaact acagtgagga
     4681 cctggagttg gcctccctgc tgatcaagat tgacattttc cctgccaagc aggagaatgg
     4741 tgacctcagt cccttcagtg gtacgtccct gcgggagcgg ggctcagatg cctcaggcca
     4801 gctgtttcat ggccgagccc gggaaggctc ctttgaatcc cgctaccagc agccgtttga
     4861 ggacttccgc atctcccagg agcatctcgc agaccatttt gacagtcgag aacgaagggc
     4921 cccaagaagg actcgggtca atggagacaa ccgcctctct agatttgggc ccaattcctg
     4981 caggcgagct ctcggctcga gcggcgtctt cacactcgaa gatttcgttg gggactggcg
     5041 acagacagcc ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt
     5101 tcagaatctc ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg
     5161 gctgaagatc gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg
     5221 ccagatcgaa aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat
     5281 cctgcactat ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg
     5341 acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct
     5401 gtggaacggc aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt
     5461 ccgagtaacc atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcgtaagg
     5521 ccgcgactct agggcatgca agctgatccg gctgctaaca aagcccgaaa ggaagctgag
     5581 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcggc cgcttcgagc
     5641 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     5701 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     5761 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     5821 ggaggttttt ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa
     5881 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5941 acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
     6001 tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     6061 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6121 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6181 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6241 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6301 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6361 ggcttttgca aaaagctcga ttcttctgac actagcgcca ccatgaagaa gcccgaactc
     6421 accgctacca gcgttgaaaa atttctcatc gagaagttcg acagtgtgag cgacctgatg
     6481 cagttgtcgg agggcgaaga gagccgagcc ttcagcttcg atgtcggcgg acgcggctat
     6541 gtactgcggg tgaatagctg cgctgatggc ttctacaaag accgctacgt gtaccgccac
     6601 ttcgccagcg ctgcactacc catccccgaa gtgttggaca tcggcgagtt cagcgagagc
     6661 ctgacatact gcatcagtag acgcgcccaa ggcgttactc tccaagacct ccccgaaaca
     6721 gagctgcctg ctgtgttaca gcctgtcgcc gaagctatgg atgctattgc cgccgccgac
     6781 ctcagtcaaa ccagcggctt cggcccattc gggccccaag gcatcggcca gtacacaacc
     6841 tggcgggatt tcatttgcgc cattgctgat ccccatgtct accactggca gaccgtgatg
     6901 gacgacaccg tgtccgccag cgtagctcaa gccctggacg aactgatgct gtgggccgaa
     6961 gactgtcccg aggtgcgcca cctcgtccat gccgacttcg gcagcaacaa cgtcctgacc
     7021 gacaacggcc gcatcaccgc cgtaatcgac tggtccgaag ctatgttcgg ggacagtcag
     7081 tacgaggtgg ccaacatctt cttctggcgg ccctggctgg cttgcatgga gcagcagact
     7141 cgctacttcg agcgccggca tcccgagctg gccggcagcc ctcgtctgcg agcctacatg
     7201 ctgcgcatcg gcctggatca gctctaccag agcctcgtgg acggcaactt cgacgatgct
     7261 gcctgggctc aaggccgctg cgatgccatc gtccgcagcg gggccggcac cgtcggtcgc
     7321 acacaaatcg ctcgccggag cgcagccgta tggaccgacg gctgcgtcga ggtgctggcc
     7381 gacagcggca accgccggcc cagtacacga ccgcgcgcta aggaggtagg tcgagtttag
     7441 actctagcca tcacgatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     7501 gttttttgtg tgaatcgata gcgataagga tcctctttgc gcttgcgttt tcccttgtcc
     7561 agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct
     7621 acgtaatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7681 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7741 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7801 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7861 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7921 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7981 tcaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8041 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8101 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8161 acactgcggc caacttactt ctgacaacta tcggaggacc gaaggagcta accgcttttt
     8221 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8281 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8341 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8401 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8461 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8521 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8581 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taattcgaaa
     8641 tgaccgacca agcgacgccc aaccggtatc agctcactca aaggcggtaa tacggttatc
     8701 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8761 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8821 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8881 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8941 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9001 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9061 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9121 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9181 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9241 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9301 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9361 cagaaaaaaa ggatttcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9421 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9481 gatcctttta tagtccggaa atacaggaac gcacgctgga tggcccttcg ctgggatggt
     9541 gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc ctgtaccctc
     9601 tggttgcata ggtattcata cggttaaaat ttatcaggcg cgattgcggc agtttttcgg
     9661 gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg
     9721 tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta gccatcgaga
     9781 taaaccgcag tactccggcc acgatgcgtc cggcgtagag gatcgagatc t
//
