LOCUS       sequence-425974-        7842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human PALB2. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_PALB2:1-1186
FEATURES             Location/Qualifiers
     source          1..7842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4905..5026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4916..4935)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(5141..5174)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5184..5231
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5263..6057
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5317..5336)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5927..5946
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(6215..6803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6295..6314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6929..6977
                     /note="synthetic polyadenylation signal"
     CDS             complement(7001..7399)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      7396..7405
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7430..7787)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7444..7579
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        7596..7787
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7744..7764
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tggacgagcc
     1261 tcccgggaag cccctcagct gtgaggagaa ggaaaagtta aaggagaaat tagcattctt
     1321 gaaaagggaa tacagcaaga cactagcccg ccttcagcgt gcccaaagag ctgaaaagat
     1381 taagcattct attaagaaaa cagtagaaga acaagattgt ttgtctcagc aggatctctc
     1441 accgcagcta aaacactcag aacctaaaaa taaaatatgt gtttatgaca agttacacat
     1501 caaaacccat cttgatgaag aaactggaga aaagacatct atcacacttg atgttgggcc
     1561 tgagtccttt aaccctggag atggcccagg aggattacct atacaaagaa cagatgacac
     1621 ccaagaacat tttccccaca gggtcagtga ccctagtggt gagcaaaagc agaagctgcc
     1681 aagcagaaga aagaagcagc agaagaggac atttatttca caggagagag actgtgtctt
     1741 tggcactgat tcactcagat tgtctgggaa aagactaaag gaacaggaag aaatcagtag
     1801 caaaaatcct gctagatcac cagtaactga aataagaact caccttttaa gtcttaaatc
     1861 tgaacttcca gattctccag aaccagttac agaaattaat gaagacagtg tattaattcc
     1921 accaactgcc caaccagaaa aaggtgttga tacattccta agaagaccta atttcaccag
     1981 ggcgactaca gttcctttac agactctatc agatagcggt agtagtcagc accttgaaca
     2041 cattcctcct aaaggtagca gtgaacttac tactcacgac ctaaaaaaca ttagatttac
     2101 ttcacctgta agtttggagg cacaaggcaa aaaaatgact gtctctacag ataacctcct
     2161 tgtaaataaa gctataagta aaagtggcca actgcccaca agttctaatt tagaggcaaa
     2221 tatttcatgt tctctaaatg aactcaccta caataactta ccagcaaatg aaaaccaaaa
     2281 cttaaaagaa caaaatcaaa cagagaaatc tttaaaatct cccagtgaca ctcttgatgg
     2341 caggaatgaa aatcttcagg aaagtgagat tctaagtcaa cctaagagtc ttagcctgga
     2401 agcaacctct cctctttctg cagaaaaaca ttcttgcaca gtgcctgaag gccttctgtt
     2461 tcctgcagaa tattatgtta gaacaacacg aagcatgtcc aattgccaga ggaaagtagc
     2521 cgtggaggct gtcattcaga gtcatttgga tgtcaagaaa aaagggttta aaaataaaaa
     2581 taaggatgca agtaaaaatt taaacctttc caatgaggaa actgaccaaa gtgaaattag
     2641 gatgtctggc acatgcacag gacaaccaag ttcaagaacc tctcagaaac ttctctcatt
     2701 aactaaagtc agctctcccg ctgggcccac tgaagataat gacttgtcta ggaaggcagt
     2761 tgcccaagca cctggtagaa gatacacagg aaaaagaaaa tcagcctgca ccccagcatc
     2821 agatcattgt gaaccacttt tgccaacttc tagcctgtcg attgttaaca ggtccaagga
     2881 agaagtcacc tcacacaaat atcagcacga aaaattattt attcaagtga aagggaagaa
     2941 aagtcgtcat caaaaagagg attccctttc ttggagtaat agtgcttatt tatccttgga
     3001 tgatgatgct ttcacggctc catttcatag ggatggaatg ctgagtttaa agcaactact
     3061 gtcttttctc agtatcacag actttcagtt acctgatgaa gactttggac ctcttaagct
     3121 tgaaaaagtg aagtcctgct cagaaaaacc agtggagccc tttgagtcaa aaatgtttgg
     3181 agagagacat cttaaagagg gaagctgtat ttttccagag gaactgagtc ctaaacgcat
     3241 ggatacagaa atggaggact tagaagagga ccttattgtt ctaccaggaa aatcacatcc
     3301 caaaaggcca aactcgcaaa gccagcatac aaagacgggc ctttcttcat ccatattact
     3361 ttatactcct ttaaatacgg ttgcgcctga tgataatgac aggcctacca cagacatgtg
     3421 ttcacctgct ttccccatct taggtactac tccagccttt ggccctcaag gctcctatga
     3481 aaaagcatct acagaagttg ctggacgaac ttgctgcaca ccccaacttg ctcatttgaa
     3541 agactcagtc tgtcttgcca gtgatactaa acaattcgac agttcaggca gcccagcaaa
     3601 accacatacc accctgcaag tgtcaggcag gcaaggacaa cctacctgtg actgtgactc
     3661 tgtcccgcca ggaacacctc cacccattga gtcattcact tttaaagaaa atcagctctg
     3721 tagaaacaca tgccaggagc tgcataaaca ttccgtcgaa cagactgaaa cagcagagct
     3781 tcctgcttct gatagcataa acccaggcaa cctacaattg gtttcagagt taaagaatcc
     3841 ttcaggttcc tgttccgtag atgtgagtgc catgttttgg gaaagagccg gttgtaaaga
     3901 gccatgtatc ataactgctt gcgaagatgt agtttctctt tggaaagctc tggatgcttg
     3961 gcagtgggaa aaactttata cctggcactt cgcagaggtt ccagtattac agatagttcc
     4021 agtgcctgat gtgtataatc tcgtgtgtgt agctttggga aatttggaaa tcagagagat
     4081 cagggcattg ttttgttcct ctgatgatga aagtgaaaag caagtactac tgaagtctgg
     4141 aaatataaaa gctgtgcttg gcctgacaaa gaggaggcta gttagtagca gtgggaccct
     4201 ttctgatcaa caagtagaag tcatgacgtt tgcagaagat ggaggaggca aagaaaacca
     4261 atttttgatg ccccctgagg agactatact aacttttgct gaggtccaag ggatgcaaga
     4321 agctctgctt ggtactacta ttatgaacaa cattgttatt tggaatttaa aaactggtca
     4381 actcctgaaa aagatgcaca ttgatgattc ttaccaagct tcagtctgtc acaaagccta
     4441 ttctgaaatg gggcttctct ttattgtcct gagtcatccc tgtgccaaag agagtgagtc
     4501 gttgcgaagc cctgtgtttc agctcattgt gattaaccct aagacgactc tcagcgtggg
     4561 tgtgatgctg tactgtcttc ctccagggca ggctggcagg ttcctggaag gtgacgtgaa
     4621 agatcactgt gcagcagcaa tcttgacttc tggaacaatt gccatttggg acttacttct
     4681 cggtcagtgt actgccctcc tcccacctgt ctctgaccaa cattggtctt ttgtgaaatg
     4741 gtcgggtaca gactctcatt tgctggctgg acaaaaagat ggaaatatat ttgtatacca
     4801 ctattcataa tctagaggtc aagcttcggg ggtttaaacc tgcaggcatg caagctgatt
     4861 tggctgctgc caccgctgag caaggccgct tcgagcagac atgataagat acattgatga
     4921 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     4981 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     5041 cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa
     5101 cctctacaaa tgtggtaaaa tcttaattaa gacttctaat ataacttcgt atagcataca
     5161 ttatacgaag ttatggttcc actgttgaca attaatcatc ggcatagtat atcggcatag
     5221 tataatacga caaggtgagg aactaaaccc aggaggcaga tcatgattga acaagatgga
     5281 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5341 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5401 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     5461 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     5521 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     5581 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5641 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     5701 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     5761 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     5821 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     5881 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     5941 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6001 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgattc
     6061 gaaatgaccg accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt
     6121 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6181 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6241 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6301 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6361 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6421 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6481 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6541 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6601 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     6661 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6721 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6781 cgcgcagaaa aaaaggattt caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6841 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6901 cctagatcct tttatagtcc ggaatcgtca cacaaaaaac caacacacag atgtaatgaa
     6961 aataaagata ttttattgcg gccatcgtga tggctagagt ttagccctcc cacacataac
     7021 cagagggcag caattcacga atcccaactg ccgtcggctg tccatcactg tccttcacta
     7081 tggctttgat cccaggatgc agatcgagaa gcacctgtcg gcaccgtccg caggggctca
     7141 agatgcccct gttctcattt ccgatcgcga cgatacaagt caggttgcca gctgccgcag
     7201 cagcagcagt gcccagcacc acgagttctg cacaaggtcc cccagtaaaa tgatatacat
     7261 tgacaccagt gaagatgcgg ccgtcgctag agagagctgc gctggcgacg ctgtagtctt
     7321 cagagatggg gatgctgttg attgtagccg ttgctctttc aatgagggtg gattcttctt
     7381 gagacaaagg cttggccatg gtggcgctag tgtcagaaga atcgagcttt ttgcaaaagc
     7441 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     7501 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     7561 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     7621 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg
     7681 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     7741 cacaccctaa ctgacacaca ttccacagct ggttctttcc gcctcagaag gtacctaacc
     7801 aagttcctct ttcagaggtt atttcaggcc atggtgctgc gc
//
