LOCUS       sequence-426010-        6315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR48. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_WDR48:1-677
FEATURES             Location/Qualifiers
     source          1..6315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3378..3499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3389..3408)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3614..3647)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3657..3704
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3736..4530
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3790..3809)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4400..4419
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4688..5276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4768..4787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5402..5450
                     /note="synthetic polyadenylation signal"
     CDS             complement(5474..5872)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5869..5878
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5903..6260)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5917..6052
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6069..6260
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6217..6237
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tggcggccca
     1261 tcaccggcag aacacagcag ggcggaggaa agtgcaggtt tcctatgtta ttcgagatga
     1321 agtggagaag tacaaccgaa atggagtcaa tgctctgcag ctggatccag cactaaatag
     1381 acttttcaca gccggtcgag actctatcat aagaatatgg agtgtcaatc agcacaagca
     1441 agatccatat atagcatcta tggaacacca tactgattgg gtaaacgaca ttgtactctg
     1501 ttgtaatggg aaaacattaa tatctgcttc ttctgacacg acagtaaaag tatggaatgc
     1561 acacaaggga ttttgcatgt caacattaag gacacataag gattacgtaa aggccttagc
     1621 atatgccaag gataaagaac tagtagcatc agctgggttg gacagacaaa tattcctttg
     1681 ggatgtgaat actctaacag cattgactgc ctcaaataac actgtcacaa cttcttcttt
     1741 aagtggaaac aaagattcca tttatagcct ggccatgaat caactgggaa caatcattgt
     1801 atcagggtcc actgaaaagg tgttacgggt atgggatcca agaacatgtg caaaactaat
     1861 gaagcttaaa gggcacacgg ataatgtgaa ggcattgcta ttaaacagag atggcacgca
     1921 atgcctgtca ggcagttctg atgggacaat tcgcctttgg tcccttggcc agcagagatg
     1981 tatagcaaca taccgagtcc atgatgaagg tgtttgggcg ctgcaagtca atgatgcctt
     2041 cacacatgtg tattctggtg gaagggacag gaagatttat tgtacagacc taagaaaccc
     2101 tgacattcgg gtgctaattt gtgaagaaaa agcaccagtt ctcaagatgg agcttgatag
     2161 atcagctgat cctcctcctg caatttgggt tgcaacaact aagtctacag taaataaatg
     2221 gactttgaaa ggaattcata attttagagc ctctggagat tatgacaatg actgtacaaa
     2281 tcctataaca cctctttgta cacaacctga ccaggttatt aaagggggtg ctagtattat
     2341 tcagtgccac attcttaatg ataagagaca tatattaacc aaagatacca ataataatgt
     2401 ggcatattgg gatgtattga aggcatgtaa agttgaagat ctgggcaaag tggattttga
     2461 agatgaaatt aagaaaagat ttaaaatggt gtatgtgcca aattggttct cagtagactt
     2521 aaaaacaggg atgttaacta ttactttgga tgaaagtgat tgttttgctg cctgggtttc
     2581 tgcaaaagat gctggtttca gcagccctga tgggtcagat ccaaaattga atttaggagg
     2641 acttttactc caagcactcc tggaatattg gcctagaaca catgtgaatc caatggatga
     2701 agaggaaaat gaagtaaacc atgtaaatgg ggagcaggag aaccgagtgc agaagggaaa
     2761 tggatatttt caagtgcccc cacatacacc cgtgatcttt ggtgaagctg gaggtcgcac
     2821 actgttcagg ctgctctgcc gagattccgg gggtgagact gagtctatgc ttcttaatga
     2881 aacagtgcca caatgggtaa ttgacatcac tgtggataaa aatatgccca aattcaacaa
     2941 aattcctttc tacctccaac ctcatgcatc ttcaggagca aaaaccttaa aaaaagatag
     3001 actctctgct agtgacatgc tccaagtccg aaaagttatg gaacatgttt atgaaaaaat
     3061 tatcaacttg gataatgagt ctcaaaccac tagctcttct aataatgaaa aaccaggaga
     3121 acaggaaaaa gaagaagata ttgctgtgtt ggcagaggag aaaattgaac ttttgtgcca
     3181 agaccaggtt ttggatccaa atatggacct tcgaacagtg aaacacttca tatggaagag
     3241 cggtggagac ctcaccctcc attaccgtca gaagtccacg taatctagag gtcaagcttc
     3301 gggggtttaa acctgcaggc atgcaagctg atttggctgc tgccaccgct gagcaaggcc
     3361 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3421 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3481 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3541 ggagatgtgg gaggtttttt taagcaagta aaacctctac aaatgtggta aaatcttaat
     3601 taagacttct aatataactt cgtatagcat acattatacg aagttatggt tccactgttg
     3661 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     3721 cccaggaggc agatcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     3781 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     3841 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     3901 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     3961 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     4021 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     4081 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     4141 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     4201 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     4261 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     4321 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     4381 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     4441 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     4501 ttctatcgcc ttcttgacga gttcttctga ttcgaaatga ccgaccaagc gacgcccaac
     4561 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4621 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4681 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4741 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4801 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4861 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4921 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4981 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5041 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5101 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     5161 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5221 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tttcaagaag
     5281 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5341 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttatag tccggaatcg
     5401 tcacacaaaa aaccaacaca cagatgtaat gaaaataaag atattttatt gcggccatcg
     5461 tgatggctag agtttagccc tcccacacat aaccagaggg cagcaattca cgaatcccaa
     5521 ctgccgtcgg ctgtccatca ctgtccttca ctatggcttt gatcccagga tgcagatcga
     5581 gaagcacctg tcggcaccgt ccgcaggggc tcaagatgcc cctgttctca tttccgatcg
     5641 cgacgataca agtcaggttg ccagctgccg cagcagcagc agtgcccagc accacgagtt
     5701 ctgcacaagg tcccccagta aaatgatata cattgacacc agtgaagatg cggccgtcgc
     5761 tagagagagc tgcgctggcg acgctgtagt cttcagagat ggggatgctg ttgattgtag
     5821 ccgttgctct ttcaatgagg gtggattctt cttgagacaa aggcttggcc atggtggcgc
     5881 tagtgtcaga agaatcgagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact
     5941 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     6001 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     6061 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     6121 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
     6181 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
     6241 gctggttctt tccgcctcag aaggtaccta accaagttcc tctttcagag gttatttcag
     6301 gccatggtgc tgcgc
//
