LOCUS       sequence-426015-        8967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human MOV10. C-terminal NanoLuc 
            luciferase tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pNLF1-C_MOV10:1-1003
FEATURES             Location/Qualifiers
     source          1..8967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3707..3718
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             4142..4654
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(4730..4748)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    4785..4906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4796..4815)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4850..4869
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5151..5508
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5151..5342
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5174..5194)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5359..5494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5539..6576
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             6555..6563
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    6600..6648
                     /note="synthetic polyadenylation signal"
     promoter        6804..6908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6909..6977,6978..7769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7127..7146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7927..8515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8007..8026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8631..8914
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcaaa gcgatcgctt ccgaattcgc
     1081 caccatgccc agtaagttca gctgccggca gctccgggag gcgggccagt gtttcgagag
     1141 tttcctggtc gttcggggac tggacatgga gacagatcgc gagcggctgc ggaccattta
     1201 taaccgcgac ttcaagatca gctttgggac ccccgcccct ggcttctcct ccatgctgta
     1261 tggaatgaag attgcaaatc tggcctacgt caccaagact cgggtcaggt tcttcagact
     1321 cgaccgctgg gccgacgtgc ggttcccaga aaagaggaga atgaagctgg ggtcagatat
     1381 cagcaaacac cacaagtcac tgctagccaa gatcttttat gacagggctg agtatcttca
     1441 tgggaaacat ggtgtggatg tggaagtcca ggggccccat gaagcccgag atgggcagct
     1501 ccttatccgc ctggatttga accgcaaaga ggtgctgacc ctgaggcttc ggaatggcgg
     1561 aacccagtct gttaccctca ctcacctctt cccactctgc cggacacccc agtttgcttt
     1621 ctacaatgaa gaccaggagt tgccctgtcc actgggcccc ggtgaatgct atgaactcca
     1681 tgtccattgt aagaccagct ttgtgggcta cttcccagcc acagtgctct gggagctgct
     1741 gggacctggg gagtcgggtt cagaaggagc cggcacattc tacattgccc gcttcttggc
     1801 tgccgtcgcc cacagccccc tggctgcaca gctgaagccc atgactccct tcaagcggac
     1861 ccggatcacc ggaaaccctg tggtgaccaa tcggatagag gaaggagaga gacctgaccg
     1921 cgctaagggc tatgacctgg agttaagtat ggcgctgggg acatactacc cacctccccg
     1981 cctcaggcag ctgctcccca tgcttcttca gggaacaagt atcttcactg cccctaagga
     2041 gatcgcagag atcaaggccc agctggagac agccctgaag tggaggaact atgaggtgaa
     2101 gctgcggctg ctgctgcacc tggaggaact gcagatggag catgatatcc ggcactatga
     2161 cctggagtcg gtgcccatga cctgggaccc tgtggaccag aaccccaggc tgctcacgct
     2221 ggaggttcct ggagtgactg agagccgccc ctcagtgcta cggggcgacc acctgtttgc
     2281 ccttttgtcc tcggagacac accaggagga ccccatcaca tataagggct ttgtgcacaa
     2341 ggtggaattg gaccgtgtca agctgagctt ttccatgagc ctcctgagcc gctttgtgga
     2401 tgggctgacc ttcaaggtga actttacctt caaccgccag ccgctgcgag tccagcaccg
     2461 tgccctggag ctgacagggc gctggctgct gtggcccatg ctctttcctg tggcacctcg
     2521 ggacgtcccg ctgctgccct cagatgtgaa actcaagctg tacgaccgga gtctggagtc
     2581 aaacccagag cagctgcagg ccatgaggca cattgttacg ggcaccaccc gtccagcccc
     2641 ctacatcatc tttgggcctc caggcaccgg caagactgtc acgttagtgg aggcaattaa
     2701 gcaggtggtg aagcacttgc ccaaagccca catcttggcc tgcgctccat ccaactcagg
     2761 ggctgaccta ctctgtcaaa ggctccgggt ccaccttcct agctccatct accgcctcct
     2821 ggcccccagc agggacatcc gcatggtacc tgaggacatc aagccctgct gcaactggga
     2881 cgcaaagaag ggggagtatg tatttcccgc caagaagaag ctgcaggaat accgggtctt
     2941 aattaccacc ctcatcactg ccggcaggtt ggtctcggcc cagtttccca ttgatcactt
     3001 cacacacatc ttcatcgatg aggctggcca ctgcatggag cctgagagtc tggtagctat
     3061 agcagggctg atggaagtaa aggaaacagg tgatccagga gggcagctgg tgctggcagg
     3121 agaccctcgg cagctggggc ctgtgctgcg ttccccactg acccagaagc atggactggg
     3181 atactcactg ctggagcggc tgctcaccta caactccctg tacaagaagg gccctgatgg
     3241 ctatgacccc cagttcataa ccaagctgct ccgcaactac aggtctcatc ccaccatcct
     3301 ggacattcct aaccagctct attatgaagg ggagctgcag gcctgtgctg atgtcgtgga
     3361 tcgagaacgc ttctgccgct gggcgggcct acctcgacag ggctttccca tcatctttca
     3421 cggcgtaatg ggcaaagatg agcgtgaagg caacagccca tccttcttca accctgaaga
     3481 ggctgccaca gtgacttcct acctgaagct gctcctggcc ccctcctcca agaagggcaa
     3541 agctcgcctg agccctcgaa gtgtgggcgt catctccccg taccggaaac aggtggagaa
     3601 aatccgttac tgcatcacca aacttgacag ggagcttcga ggactggatg acatcaagga
     3661 cttgaaggtg ggttcagtag aagaattcca aggccaagaa cgaagcgtca tcctcatctc
     3721 caccgtgcga agcagccaga gctttgtgca gctggatctg gactttaatc tgggtttcct
     3781 taagaacccc aagaggttca atgtagctgt gacccgggcc aaggccctgc tcatcatcgt
     3841 ggggaacccc cttctcctgg gccatgaccc tgactggaaa gtattcctgg agttctgtaa
     3901 agaaaacgga gggtataccg ggtgtccctt ccctgccaaa ctggacctgc aacagggaca
     3961 gaatttactg caaggtctga gcaagctcag cccctctacc tcagggcccc acagccatga
     4021 ctacctcccc caggagcggg agggtgaagg gggcctgtct ctgcaagtgg agccagagtg
     4081 gaggaatgag ctctctagat ttgggcccaa ttcctgcagg cgagctctcg gctcgagcgg
     4141 cgtcttcaca ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga
     4201 ccaagtcctt gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac
     4261 tccgatccaa aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat
     4321 catcccgtat gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt
     4381 ggtgtaccct gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat
     4441 cgacggggtt acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt
     4501 gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga
     4561 cgagcgcctg atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac
     4621 cggctggcgg ctgtgcgaac gcattctggc gtaaggccgc gactctaggg catgcaagct
     4681 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     4741 taactagcat aaccccttgg ggcggccgct tcgagcagac atgataagat acattgatga
     4801 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     4861 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     4921 cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa
     4981 cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca atttcctgat
     5041 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg gatctgcgca
     5101 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
     5161 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5221 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5281 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5341 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5401 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5461 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcgattct
     5521 tctgacacta gcgccaccat gaagaagccc gaactcaccg ctaccagcgt tgaaaaattt
     5581 ctcatcgaga agttcgacag tgtgagcgac ctgatgcagt tgtcggaggg cgaagagagc
     5641 cgagccttca gcttcgatgt cggcggacgc ggctatgtac tgcgggtgaa tagctgcgct
     5701 gatggcttct acaaagaccg ctacgtgtac cgccacttcg ccagcgctgc actacccatc
     5761 cccgaagtgt tggacatcgg cgagttcagc gagagcctga catactgcat cagtagacgc
     5821 gcccaaggcg ttactctcca agacctcccc gaaacagagc tgcctgctgt gttacagcct
     5881 gtcgccgaag ctatggatgc tattgccgcc gccgacctca gtcaaaccag cggcttcggc
     5941 ccattcgggc cccaaggcat cggccagtac acaacctggc gggatttcat ttgcgccatt
     6001 gctgatcccc atgtctacca ctggcagacc gtgatggacg acaccgtgtc cgccagcgta
     6061 gctcaagccc tggacgaact gatgctgtgg gccgaagact gtcccgaggt gcgccacctc
     6121 gtccatgccg acttcggcag caacaacgtc ctgaccgaca acggccgcat caccgccgta
     6181 atcgactggt ccgaagctat gttcggggac agtcagtacg aggtggccaa catcttcttc
     6241 tggcggccct ggctggcttg catggagcag cagactcgct acttcgagcg ccggcatccc
     6301 gagctggccg gcagccctcg tctgcgagcc tacatgctgc gcatcggcct ggatcagctc
     6361 taccagagcc tcgtggacgg caacttcgac gatgctgcct gggctcaagg ccgctgcgat
     6421 gccatcgtcc gcagcggggc cggcaccgtc ggtcgcacac aaatcgctcg ccggagcgca
     6481 gccgtatgga ccgacggctg cgtcgaggtg ctggccgaca gcggcaaccg ccggcccagt
     6541 acacgaccgc gcgctaagga ggtaggtcga gtttagactc tagccatcac gatggccgca
     6601 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga
     6661 taaggatcct ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca
     6721 tccggggtca gcaccgtttc tgcggactgg ctttctacgt aatggtttct tagacgtcag
     6781 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6901 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6961 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7021 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7081 ttcgccccga agaacgtttt ccaatgatga gcactttcaa agttctgcta tgtggcgcgg
     7141 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7201 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7261 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7321 caactatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7381 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7441 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7501 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7561 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7621 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7681 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7741 taggtgcctc actgattaag cattggtaat tcgaaatgac cgaccaagcg acgcccaacc
     7801 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7861 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7921 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7981 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8041 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8101 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8161 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8221 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8281 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8341 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8401 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8461 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ttcaagaaga
     8521 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8581 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttatagt ccggaaatac
     8641 aggaacgcac gctggatggc ccttcgctgg gatggtgaaa ccatgaaaaa tggcagcttc
     8701 agtggattaa gtgggggtaa tgtggcctgt accctctggt tgcataggta ttcatacggt
     8761 taaaatttat caggcgcgat tgcggcagtt tttcgggtgg tttgttgcca tttttacctg
     8821 tctgctgccg tgatcgcgct gaacgcgttt tagcggtgcg tacaattaag ggattatggt
     8881 aaatccactt actgtctgcc ctcgtagcca tcgagataaa ccgcagtact ccggccacga
     8941 tgcgtccggc gtagaggatc gagatct
//
