LOCUS       sequence-42602-e        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, 
            Howarth M
  TITLE     Peptide tag forming a rapid covalent bond to a protein, through 
            engineering a bacterial adhesin.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 
            24.
  PUBMED    22366317
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-03-20"; volume: "109(12)"; pages: 
            "E690-7. Epub 2012 Feb 24"
COMMENT     Sequence Label: pDEST14-SpyCatcher
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             99..437
                     /codon_start=1
                     /product="truncated version of the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyCatcher
                     /note="forms a covalent isopeptide bond with the SpyTag 
                     peptide (Zakeri et al., 2012)"
                     /translation="VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATME
                     LRDSSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNG
                     KATKGDAHI"
     CDS             502..519
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(572..590)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      586..633
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(757..776)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     967..988
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttaagaagg agatatacat atgtcgtact accatcacca tcaccatcac gattacgaca
       61 tcccaacgac cgaaaacctg tattttcagg gcgccatggt tgatacctta tcaggtttat
      121 caagtgagca aggtcagtcc ggtgatatga caattgaaga agatagtgct acccatatta
      181 aattctcaaa acgtgatgag gacggcaaag agttagctgg tgcaactatg gagttgcgtg
      241 attcatctgg taaaactatt agtacatgga tttcagatgg acaagtgaaa gatttctacc
      301 tgtatccagg aaaatataca tttgtcgaaa ccgcagcacc agacggttat gaggtagcaa
      361 ctgctattac ctttacagtt aatgagcaag gtcaggttac tgtaaatggc aaagcaacta
      421 aaggtgacgc tcatatttaa atggttgatg cttgaggatc cgaattcgag ctccgtcgac
      481 aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa
      541 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
      601 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg
      661 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      721 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      781 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      841 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      901 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
      961 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtc
//
