LOCUS       sequence-426030-        8102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f, CCR5TC 
            zinc finger, and KRAB(A) transcriptional repressor domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Rimbault C, Breillat C, Compans B, Toulme E, Nunes Vicente F, 
            Fernandez-Monreal M, Mascalchi P, Genuer C, Puente-Munoz V, 
            Gauthereau I, Hosy E, Claverol S, Giannone G, Chamma I, Mackereth 
            CD, Poujol C, Choquet D, Sainlos M
  TITLE     Engineering paralog-specific PSD-95 recombinant binders as minimally
            interfering multimodal probes for advanced imaging techniques.
  JOURNAL   Elife. 2024 Jan 3;13:e69620. doi: 10.7554/eLife.69620.
  PUBMED    38167295
REFERENCE   2  (bases 1 to 8102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-01-3"; volume: "13:e69620. doi"; pages: " 
            10.7554/eLife.69620"
COMMENT     Sequence Label: pCAG_Xph20-GCaMP7f-CCR5TC
FEATURES             Location/Qualifiers
     source          1..8102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..21
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             396..452
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     665..686
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(792..813)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1053..1072)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1188..1628
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKELFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             1710..2051
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     CDS             2100..2225
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     intron          2274..2406
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2389..2408
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    2415..3003
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2468..2488)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2886..2897)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3030..3047)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3036..3147
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3243..3263)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3243..3261)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3282..3298)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3404..3423)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3469..3524
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3523..3542)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3885..3901)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3909..3925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3933..3939,3940..3957,3958..3963))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3978..3999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4058..4253
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4104..4239
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4259..4393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4296..4315)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4350..4369
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4461..4478)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4632..5220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4712..4731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5391..6182,6183..6251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6014..6033
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6252..6356)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    6407..6418
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     enhancer        6422..6801
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6803..7079
                     /label=chicken beta-actin promoter
     intron          7080..8096
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     8020..8042
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcgg taccgcgggc
       61 ccggggccac catggcgggc agctctgtca gttccgtgcc gaccaaactg gaagtggttg
      121 cggccacccc gacgtctctg ctgatcagct gggatgcagt ggcgaagaat gtgaaggtcg
      181 gttattaccg tatcacctat ggcgaaacgg gcggtaactc cccggttcag gaattcaccg
      241 tcccgggtag ttcctcaacc gcaacgattt ccggcctgtc accgggtgtc gactatacca
      301 ttacggttta cgctaatggg gtgctgagta agatggtgct tccgatttct atcaattacc
      361 gtaccggtag cggctccagt agatctgact catcacgtcg taagtggaat aagacaggtc
      421 acgcagtcag agctataggt cggctgagct cactcgagaa cgtctatatc aaggccgaca
      481 agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcggcg
      541 tgcagctcgc ctaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      601 ccgacaacca ctacctgagc gtgcagtcca aactttcgaa agaccccaac gagaagcgcg
      661 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
      721 tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg
      781 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      841 gcgagggtga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      901 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      961 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1021 gctacatcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1081 aggtgaagtt cgagggcgac accctggtga accgcatcga gcttaagggc atcgacttca
     1141 aggaggacgg caacatcctg gggcacaagc tggagtacaa cctgccggac caactgactg
     1201 aagagcagat cgcagaattt aaagagctgt tctccctatt tgacaaggac ggggatggga
     1261 caataacaac caaggagctg gggacggtga tgcggtctct ggggcagaac cccacagaag
     1321 cagagctgca ggacatgatc aatgaagtag atgccgacgg tgacggcaca atcgacttcc
     1381 ctgagttcct gacaatgatg gcaagaaaaa tgaaatacac agacagtgaa gaagaaatta
     1441 gagaagcgtt ccgtgtgttt gataaggatg gcaatggcta catcagtgca gcagagcttc
     1501 gccacgtgat gacaaacctt ggagagaagt taacagatga agaggttgat gaaatgatca
     1561 gggaagcaga catcgatggg gatggtcagg taaactacga agagtttgta caaatgatga
     1621 cagcgaaggg cggttcgggc ggtagcggcg gttcgggcgg tagcgctagc ggagctggcg
     1681 ctggagcggg tgcaggggct ggctctagat tccagtgccg gatctgcatg cggaacttca
     1741 gcgaccggtc caacctgagc aggcacatca gaacccacac cggagaaaag cccttcgcct
     1801 gcgacatttg cggccggaag ttcgccatca gcagcaacct gaacagccac accaagatcc
     1861 acactggcag ccagaaacct ttccagtgca gaatttgtat gagaaacttt agcagaagcg
     1921 acaacctggc cagacacatc cggacacata ctggtgaaaa accttttgcc tgtgatatct
     1981 gtggcagaaa gtttgccacc tccggcaatc tgacccggca cacaaagatt cacctgcggg
     2041 gcagccagct atcgattgtc gacgctcctg aacaacgtga aggtgcttct caagtttctg
     2101 tttctgttac ttttgaagat gttgctgttc tttttactcg tgatgaatgg aaaaaacttg
     2161 atctttctca acgttctctt tatcgtgaag ttatgcttga aaattattct aatcttgctt
     2221 ctatggctta aacgcgttgt acaagtaaag cggccccgga ctcgaggccg caggtaagta
     2281 tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa
     2341 gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt gcctttctct
     2401 ccacaggtgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2461 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2521 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2581 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2641 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2701 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2761 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     2821 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     2881 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     2941 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3001 cgcctggaat tcgagctcgg tacgatcagc ctcgactgtg ccttctagtt gccagccatc
     3061 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     3121 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     3181 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagcc cagcttttgt
     3241 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     3301 tgaaattgtt atccgctaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     3361 tggtgtggcc aatgccctgg ctcacaaata ccactgagat cgatcttttt ccctctgcca
     3421 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     3481 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     3541 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     3601 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     3661 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     3721 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     3781 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     3841 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     3901 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3961 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4021 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca
     4081 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4141 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4201 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa
     4261 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4321 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4381 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     4441 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     4501 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4561 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4621 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4681 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4741 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4801 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4861 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4921 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4981 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5041 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     5101 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5161 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5221 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5281 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     5341 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5401 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     5461 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     5521 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5581 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5641 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5701 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5761 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5821 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5881 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5941 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6001 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6061 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6361 tttccccgaa aagtgccacc tgggtcgaca ttgattattg actagagtca tcctcatcgt
     6421 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     6481 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     6541 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     6601 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     6661 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     6721 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     6781 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
     6841 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
     6901 atgggggcgg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
     6961 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     7021 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     7081 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     7141 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     7201 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
     7261 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
     7321 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     7381 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     7441 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     7501 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     7561 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     7621 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     7681 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     7741 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     7801 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     7861 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     7921 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     7981 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     8041 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     8101 tg
//
