LOCUS       sequence-42604-e        2301 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Yoo SK, Starnes TW, Deng Q, Huttenlocher A
  TITLE     Lyn is a redox sensor that mediates leukocyte wound attraction in 
            vivo.
  JOURNAL   Nature. 2011 Nov 20;480(7375):109-12. doi: 10.1038/nature10632.
  PUBMED    22101434
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-11-20- 20"; volume: "480"; issue: "7375"; 
            pages: "109-12"
COMMENT     Sequence Label: tol2-mpx-lyn C466A-GFP
FEATURES             Location/Qualifiers
     source          1..2301
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1576..1585
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1582..1584,1585..1587,1588..2301)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1582..1584,1585..1587,1588..2298)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1627..1648)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1888..1907)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2235..2256
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgggctgta agaaatcaaa gctggatgga gatcagaatg gatgcgtgct ggaacctgtg
       61 aataaggaac accccgtcag aaccgaccag acatatgtga gggacccaac ttcatacaag
      121 ccccctatca ccagccagaa cgctggactg ctgccaggac aggtgttcca gaaaatggag
      181 gaaaaggaga aaattgtggt cgcactgtac ccctatgaag ccatccacgc tgacgatctg
      241 ggctttaaga aaggggagaa gctgaaaatc attgaggaac atggggaatg gtggaaggcc
      301 aggtccctga ccacacgcaa agagggattc attccttcta actatgtggc agaggccgat
      361 accatggaaa cagaggaatg gttttacaag gacatcacac gaaaagatgc tgagcggcag
      421 ctgctggctc ccgcaaataa gcctggctcc tatctgattc gcgagtcaga aactagcaaa
      481 gggtcataca gcctgtccat ccgagacgtg gatgcccagg ggcaggacgt ggtcaagcac
      541 tataaaatcc gcagtctgga taatggcggg tactatatct ctccaaagat tactttcagt
      601 gacatcagct ccatgattaa gcactaccat aaacagtccg acggcctgtg cagaaagctg
      661 gataaagcct gtgagaagcc taaagctcag aagccatggg acaaagatgc ctgggaaatc
      721 tccaaggata gcatcaagat ggtgaagaaa ctgggagcag gccagttcgg agaggtctgg
      781 atggcctttt ataacaatag tacaaaggtg gctgtcaaga ctctgaaacc cggcaccatg
      841 tcagtggagg catttctgga ggaagccaac ctgatgaaga ctctgcagca cgaccggctg
      901 gtcagactgt acgccgtggt cacaaaaact gagcctatct acatcattac cgaatatatg
      961 gcaaatgggt ccctgctgga cttcctgaag agtcaggccg gatcaaagat ccagctgcca
     1021 aaactgattg atttttctgc acagatcgcc gagggcatgg cctacatcga aaagaaaaac
     1081 tacatccata gggacctgcg cgccgctaat gtgctggtct ctgagatgct gctgtgcaag
     1141 attgctgatt tcggcctggc aagagtgatc gaggacgatc agtataccgc cagggaaggg
     1201 gctaagtttc ccatcaagtg gaccgctcct gaggcaatta actacggaag cttcacaatc
     1261 aagagcgata tgtggtcctt tggcgtgctg ctgtacgaaa tcattacata tggaaagatc
     1321 ccataccccg gcatgtctaa tagtgaagtg atgtctagtg tccagcgagg ctatcggatg
     1381 cctagaccag aaaacgctcc tgtggagctg tacgaaatta tgactacctg ttggaagaac
     1441 aagccagagg accggcccac cttcgattac atccagagcg tcctggatga cttttacacc
     1501 gcaaccgagg ggcagtatca gcagcagcca cgaattctgc agtcgacggt accgcgggcc
     1561 cgggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     1621 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1681 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1741 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     1801 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1861 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1921 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1981 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2041 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2101 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2161 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2221 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2281 atggacgagc tgtacaagta a
//
