LOCUS       sequence-426048-        6275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human SMU1. N-terminal 6X His tag 
            with thrombin protease cleavage site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-LIC_SMU1:1-513
FEATURES             Location/Qualifiers
     source          1..6275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      32..486
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(119..138)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     328..349
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        512..616
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(617..685,686..1477)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(835..854)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1648..2236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2137..2156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2390..2407
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2541..2765
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2832..3365)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3535..3540,3541..3557,3558..3563))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3915..4006
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3931..3948
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4074..4091
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4101..4118
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     polyA_signal    5795..5929
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5832..5851)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5886..5905
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5958..6123
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
       61 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      121 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      181 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
      241 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
      301 ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga acaacactca
      361 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
      421 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
      481 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      541 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      601 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      661 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      721 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      781 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      841 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      901 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      961 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1021 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1081 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1141 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1201 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1261 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1321 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1381 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1441 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1501 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1561 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1621 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1681 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1741 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1801 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1861 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1921 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1981 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2041 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2101 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2161 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2221 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2281 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2341 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2401 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2461 ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat
     2521 ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat
     2581 ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca
     2641 gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc
     2701 tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg gtaaagacta
     2761 tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct
     2821 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
     2881 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
     2941 atcacataag caccaagcgc gttggcctca tgcttgagca gattgatgag cgcggtggca
     3001 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc
     3061 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg
     3121 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc
     3181 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca
     3241 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt
     3301 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat
     3361 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
     3421 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     3481 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
     3541 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
     3601 cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag
     3661 cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc
     3721 aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg
     3781 cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc
     3841 gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct acgtatactc
     3901 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     3961 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     4021 ataccgtccc accatcgggc gcggatctcg gtccgaaaac catgggcagc agccatcatc
     4081 atcatcatca cagcagcggc ctggttccgc gtggtagtat gtcgatcgaa atcgaatctt
     4141 cggatgtgat ccgccttatt atgcagtact tgaaggagaa cagtttacat cgggcgttag
     4201 ccaccttgca ggaggagact actgtgtctc tgaatactgt ggacagcatt gagagttttg
     4261 tggctgacat taacagtggc cattgggata ctgtgttgca ggctatacag tctctgaaat
     4321 tgccagacaa aaccctcatt gacctctatg aacaggttgt tctggaattg atagagctcc
     4381 gtgaattggg tgctgccagg tcacttttga gacagactga tcccatgatc atgttaaaac
     4441 aaacacagcc agagcgatat attcatctgg agaacctttt ggccaggtct tactttgatc
     4501 ctcgtgaggc atacccagat ggaagtagca aagaaaagag aagagcagca attgcccagg
     4561 ccttagctgg cgaagtcagt gtggtgcctc catctcgtct catggcattg ctgggacagg
     4621 cactgaagtg gcagcagcat cagggattgc ttcctcctgg tatgaccata gatttgtttc
     4681 gaggcaaggc agctgtcaaa gatgtggaag aagaaaagtt tcctacacaa ctgagcaggc
     4741 atattaagtt tggtcagaaa tcacatgtgg agtgtgctcg attttctcca gatggtcagt
     4801 atttggtcac tgggtctgtt gatggattca ttgaagtatg gaactttact actggaaaaa
     4861 tcagaaagga tcttaagtac caggcccaag ataactttat gatgatggat gatgctgtcc
     4921 tctgcatgtg tttcagcaga gatacagaaa tgttagcaac tggggcccaa gatggaaaaa
     4981 tcaaggtgtg gaagattcag agtggacaat gtttaaggag atttgagagg gcacacagta
     5041 agggtgtcac ctgtctaagc ttttctaagg atagcagtca gatccttagt gcttcttttg
     5101 accagacaat tagaattcat ggtttaaaat ctgggaaaac cctgaaggaa tttcgtggcc
     5161 attcctcctt tgttaacgaa gcaacattta cacaagatgg acattacatt attagtgcat
     5221 cctctgatgg cactgtaaag atctggaata tgaagaccac agaatgttca aataccttta
     5281 aatccctggg cagcaccgca gggacagata ttaccgtcaa cagtgtgatt ctacttccta
     5341 aaaaccctga gcactttgtg gtgtgcaaca gatcaaacac ggtggtcatc atgaacatgc
     5401 aggggcagat tgtcagaagc ttcagttctg gtaaaagaga aggtggggac tttgtttgct
     5461 gtgccctctc tccccgtggt gaatggatct actgtgtagg ggaggacttt gtgctctact
     5521 gtttcagtac agtcactggc aaactggaga gaactttgac agtgcacgag aaggatgtga
     5581 ttggtattgc acatcaccct catcagaacc tgattgctac ctacagtgaa gatggactcc
     5641 taaagctctg gaaaccatga tgacgaagct tgtcgagaag tactagagga tcataatcag
     5701 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     5761 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     5821 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5881 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgat
     5941 atcgcctagg agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     6001 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     6061 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     6121 acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg
     6181 acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct
     6241 cttcgttatt aatgtttgta attgactgaa tatca
//
