LOCUS       sequence-426068-        7701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of the truncated variants of the SHIP2 without 
            phosphatase domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Fafilek B, Balek L, Bosakova MK, Varecha M, Nita A, Gregor T, 
            Gudernova I, Krenova J, Ghosh S, Piskacek M, Jonatova L, Cernohorsky
            NH, Zieba JT, Kostas M, Haugsten EM, Wesche J, Erneux C, Trantirek 
            L, Krakow D, Krejci P
  TITLE     The inositol phosphatase SHIP2 enables sustained ERK activation 
            downstream of FGF receptors by recruiting Src kinases.
  JOURNAL   Sci Signal. 2018 Sep 18;11(548):eaap8608. doi: 
            10.1126/scisignal.aap8608.
  PUBMED    30228226
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2018-09-18"; volume: "11(548):eaap8608. doi"; 
            pages: " 10.1126/scisignal.aap8608"
COMMENT     Sequence Label: SHIP2-Delta-PS
FEATURES             Location/Qualifiers
     source          1..7701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3861..3902
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3912..3929
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3958..3974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3958..3974)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3985..4607
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4184..4203)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(4756..5344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4836..4855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5673..5720
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5745..5764
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5952..6746)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5952..6746)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6063..6082)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6673..6692
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6781..7138)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6795..6930
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6947..7138
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7095..7115
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(7140..7244)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7271..7699
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7358..7377)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7568..7589
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcctcggcc tgcggggcgc cgggcccggg gggcgccctg ggcagccagg
     1081 ccccctcctg gtaccaccgc gacctgagcc gggcggccgc ggaggagctg ctggcccggg
     1141 cgggccgcga tggcagcttc ctggtccgag acagcgagag cgtggcgggg gccttcgcgc
     1201 tctgcgtcct gtatcagaag catgtgcaca cgtatcgcat tctgcctgat ggagaagatt
     1261 tcttggctgt gcagacctcg cagggtgtgc ctgtgcgccg cttccagacc ctgggtgagc
     1321 tcatcggcct gtacgcccag cccaaccagg gccttgtgtg cgccctgctt cttcctgtag
     1381 agggtgagcg agagccggac ccaccggatg accgggatgc ctcagatggg gaggatgaga
     1441 agcccccgct gcccccgcgc tctggctcca ccagcatttc tgcccccact gggcccagca
     1501 gtcccctgcc agctcctgag actcccacag ctccagctgc tgagagtgct cccaatgggc
     1561 tgagcaccgt ctcgcacgac tacctgaaag gcagctatgg gctggacctg gaagctgtga
     1621 ggggtggagc cagccacctg ccccacctca cccgtaccct cgctacctca tgccggaggc
     1681 tgcacagtga ggtggacaag gtcctgtcag gcctggagat cctgtccaag gtgtttgacc
     1741 agcagagctc gcccatggtg acccgccttt tgcagcagca gaacctgcca cagacagggg
     1801 agcaggaact agagagcctg gtgctgaagc tgtcagtgct aaaggacttc ctgtcaggca
     1861 tccagaagaa ggccctgaag gccctacagg acatgagctc cacagcaccc ccagctccgc
     1921 agccatccac acgtaaggcc aagaccatcc ccgtgcaggc ctttgaggtg aagctagatg
     1981 tgaccctggg tgacctgacc aagattggga agtcacagaa gttcacgctg agcgtggatg
     2041 tggagggtgg gcggctggtg ctgctgcgga gacagcggga ctcccaggag gactggacca
     2101 ccttcacgca cgaccgcatc cgccagctca ttaagtccca gcgtgtccag aacaagctgg
     2161 gtgttgtgtt tgagaaggag aaggaccgga ctcagcgcaa ggacttcatc tttgtcagtg
     2221 cccggaagcg ggaggccttc tgccagctgt tgcagctcat gaagaacaag cactccaagc
     2281 agtcaaagac ttcagaccag gcctacattg agtttgagag catcgaggcc attgtgaaga
     2341 cagccagccg caccaagttc ttcatcgagt tctactctac ctgcctggag gaatacaaga
     2401 agagctttga gaatgatgcc cagagcagtg acaacatcaa cttcctcaaa gtgcagtggt
     2461 cttcacgcca gctgcccacg ctcaaaccaa ttctggctga tatcgagtac ctgcaggacc
     2521 agcacctcct gctcacagtc aagtccatgg atggctatga atcctatggg gagtgtgtgg
     2581 ttgcactcaa atccatgatc ggcagcacgg cccaacagtt cctgaccttc ctatcccacc
     2641 gtggcgagga gacaggcaat atcagaggct ccatgaaggt gcgggtgccc acggagcgcc
     2701 tgggcacccg tgagcggctc tacgagtgga tcagcattga taaggatgag gcaggagcaa
     2761 agagcaaagc cccctctgtg tcccgaggga gccaggagcc caggtcaggg agccgcaagc
     2821 cagccttcac agaggcctcc tgcccgctct ccaggttatt tgaagaacca gagaaaccgc
     2881 caccaacggg gaggccccca gccccacccc gagcagctcc ccgggaggag cccttgaccc
     2941 ccaggttgaa gccagaggga gctcctgaac cagaaggggt ggcggccccc ccacccaaga
     3001 acagcttcaa taaccctgcc tactacgtcc ttgaaggggt cccgcaccag ctgctgcccc
     3061 cggagccacc ctcgcctgcc agggcccctg tcccatctgc caccaagaac aaagtggcca
     3121 ttacagtgcc tgctccacag cttgggcacc accggcaccc tcgtgtggga gaggggagtt
     3181 cttcagatga ggagtctgga ggcacactgc cccctccaga ctttccacct ccaccactgc
     3241 cggactcagc catcttcctg ccccccagcc tggatccttt accagggcca gtggtccggg
     3301 gccgtggtgg ggctgaggcc cgtggcccac cacctcccaa ggcccatcca aggcctccac
     3361 tgcccccagg cccctcacca gccagcactt tcctggggga agtggccagt ggggatgacc
     3421 ggtcctgctc ggtgctgcag atggccaaga cgctgagcga ggtggactat gcccctgctg
     3481 ggcctgcacg ctcagcgctc ctcccaggcc ccctggagct gcagcccccc cggggactgc
     3541 cctcggacta tggccggccc ctcagcttcc ctccaccccg catccgggag agcatccagg
     3601 aagacctggc agaggaggct ccgtgcctgc agggcgggcg ggccagcggg ctgggcgagg
     3661 caggcatgag tgcctggctg cgggccatcg gcttggagcg ctatgaggag ggcctggtgc
     3721 ataatggctg ggacgacctg gagtttctca gtgacatcac cgaggaggac ttggaggagg
     3781 ctggggtgca ggacccggct cacaagcgcc tccttctgga caccctgcag ctcagcaaga
     3841 cgcgtacgcg gccgctcgag ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     3901 cgcgtaccgg tcatcatcac catcaccatt gagctagcgt ttaaacggcc ggccgcggtc
     3961 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc
     4021 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     4081 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     4141 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     4201 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     4261 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     4321 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     4381 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     4441 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag
     4501 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc
     4561 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca
     4621 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4681 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4741 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4801 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4861 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4921 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4981 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5041 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5101 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5161 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     5221 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5281 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5341 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5401 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5461 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc
     5521 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
     5581 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
     5641 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
     5701 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
     5761 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     5821 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     5881 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     5941 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     6001 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     6061 gcaatatcac gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca
     6121 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     6181 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
     6241 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     6301 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     6361 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     6421 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     6481 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     6541 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg
     6601 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     6661 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     6721 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc
     6781 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     6841 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     6901 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     6961 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     7021 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     7081 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga
     7141 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7201 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7261 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7321 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7381 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7441 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7501 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7561 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7621 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7681 caaaaattta acgcgaattt t
//
