LOCUS       sequence-426075-        5801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human WDR91 fragment P392 to A747. N-terminal 
            6X His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_WDR91:392-747
FEATURES             Location/Qualifiers
     source          1..5801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(434..439,440..456,457..462))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        814..905
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     830..847
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             964..981
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             994..1014
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2220..2354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2257..2276)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2311..2330
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2383..2548
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2732..3186
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2819..2838)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3028..3049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3212..3316
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3317..3385,3386..4177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3535..3554)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4348..4936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4837..4856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5090..5107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5241..5465
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(5532..5801,1..264))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt
       61 cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg
      121 accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt
      181 gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt
      241 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
      301 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac
      361 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
      421 ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat
      481 ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct
      541 gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc
      601 cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg
      661 aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat
      721 cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag
      781 tggttggcta cgtatactcc ggaatattaa tagatcatgg agataattaa aatgataacc
      841 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
      901 taaatattcc ggattattca taccgtccca ccatcgggcg cggatctcgg tccgaaaacc
      961 atgcatcatc atcatcatca cagcagcggc agagaaaact tgtatttcca gggccccgag
     1021 cagcccttta ttgtgctggg acaggaggag tacggggaac accactcatc catcatgcac
     1081 tgcagagtgg actgctctgg gaggagagtc gccagcttag acgtagatgg ggtcatcaaa
     1141 gtgtggtcct tcaaccccat catgcagacc aaagcatcct ccatttccaa atcaccgctg
     1201 ctgtctttgg aatgggccac caaacgggac agactgctct tgctgggcag tggtgtggga
     1261 acagtgcgtc tctatgacac ggaagccaag aagaatctct gtgaaatcaa tatcaacgac
     1321 aacatgccca gaatcctgtc tcttgcgtgc agccccaacg gggcctcttt cgtctgttcg
     1381 gcagcagctc cgagcctcac ttcccaggtg gacttctcag caccagacat cggcagcaag
     1441 ggcatgaacc aggttcctgg caggctgctg ctgtgggaca cgaaaaccat gaagcagcag
     1501 ctccagttct ccctggatcc agaacccatt gctatcaact gtacagcctt caatcacaac
     1561 gggaacctgc tggtcacagg ggcagctgat ggcgtcatcc ggctgtttga catgcagcag
     1621 catgagtgcg cgatgagctg gagggcccac tacggggagg tctactctgt ggagttcagc
     1681 tatgatgaga acaccgtgta cagcatcggc gaggacggga agttcatcca gtggaacatc
     1741 cacaagagtg gcctcaaggt atccgagtac agcctcccct cagatgccac gggccccttt
     1801 gtgctgtctg gatacagcgg ctacaagcag gttcaagtcc ccaggggccg actcttcgct
     1861 tttgactcgg agggaaatta catgctgaca tgttctgcca caggcggcgt catctacaag
     1921 ctgggtggcg atgagaaggt tctggagagc tgcttgagcc taggtggcca ccgagcccct
     1981 gtggtcaccg tggactggag cactgccatg gactgtggga cctgcctcac cgcctccatg
     2041 gatggcaaga tcaagctgac caccctcctg gcccataaag cctgatgacg aagcttgtcg
     2101 agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     2161 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     2221 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     2281 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     2341 atcatgtctg gatctgatca ctgatatcgc ctaggagatc cgaaccagat aagtgaaatc
     2401 tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt
     2461 tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc
     2521 cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa
     2581 tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt ttctctgtca
     2641 cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga ctgaatatca
     2701 acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     2761 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     2821 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     2881 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     2941 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3001 ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga acaacactca
     3061 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3121 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     3181 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3241 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3301 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3361 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3421 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3481 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3541 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3601 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3661 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3721 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3781 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3841 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3901 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3961 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4021 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4081 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4141 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4201 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4261 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5101 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     5161 ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat
     5221 ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat
     5281 ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca
     5341 gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc
     5401 tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg gtaaagacta
     5461 tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct
     5521 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
     5581 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
     5641 atcacataag caccaagcgc gttggcctca tgcttgagca gattgatgag cgcggtggca
     5701 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc
     5761 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc c
//
