LOCUS       sequence-426078-        7800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of the truncated variants of the SHIP2 without first 
            proline-rich domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Fafilek B, Balek L, Bosakova MK, Varecha M, Nita A, Gregor T, 
            Gudernova I, Krenova J, Ghosh S, Piskacek M, Jonatova L, Cernohorsky
            NH, Zieba JT, Kostas M, Haugsten EM, Wesche J, Erneux C, Trantirek 
            L, Krakow D, Krejci P
  TITLE     The inositol phosphatase SHIP2 enables sustained ERK activation 
            downstream of FGF receptors by recruiting Src kinases.
  JOURNAL   Sci Signal. 2018 Sep 18;11(548):eaap8608. doi: 
            10.1126/scisignal.aap8608.
  PUBMED    30228226
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2018-09-18"; volume: "11(548):eaap8608. doi"; 
            pages: " 10.1126/scisignal.aap8608"
COMMENT     Sequence Label: SHIP2-Delta-PR1
FEATURES             Location/Qualifiers
     source          1..7800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2101..2112)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3960..4001
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4011..4028
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4057..4073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4057..4073)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4084..4706
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4283..4302)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(4855..5443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4935..4954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5772..5819
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5844..5863
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6051..6845)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6051..6845)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6162..6181)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6772..6791
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6880..7237)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6894..7029
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        7046..7237
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7194..7214
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(7239..7343)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7370..7798
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7457..7476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7667..7688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcctcggcc tgcggggcgc cgggcccggg gggcgccctg ggcagccagg
     1081 ccccctcctg gtaccaccgc gacctgagcc gggcggccgc ggaggagctg ctggcccggg
     1141 cgggccgcga tggcagcttc ctggtccgag acagcgagag cgtggcgggg gccttcgcgc
     1201 tctgcgtcct gtatcagaag catgtgcaca cgtatcgcat tctgcctgat ggagaagatt
     1261 tcttggctgt gcagacctcg cagggtgtgc ctgtgcgccg cttccagacc ctgggtgagc
     1321 tcatcggcct gtacgcccag cccaaccagg gccttgtgtg cgccctgctt cttcctgtag
     1381 agggtgagcg agagatgaag aacaagcact ccaagcagga cgagcccgac atgatctcag
     1441 tcttcatagg cacctggaac atgggaagtg taccacctcc aaaaaacgtg acatcctggt
     1501 tcacatcgaa gggtctgggg aagaccctgg acgaggtcac agtgaccata ccccatgaca
     1561 tctatgtctt tgggacccag gagaactcag tgggcgaccg cgagtggctg gacctactgc
     1621 gcgggggcct caaggagctt acggatctgg attaccgccc gattgccatg caatcactgt
     1681 ggaatatcaa ggtggcagtg ctggtcaagc cagagcacga gaaccgtatc agccatgtca
     1741 gtacgtccag tgtgaagact ggcatcgcca acaccctggg gaacaagggg gctgtgggcg
     1801 tctccttcat gtttaatggc acctcatttg gctttgtgaa ttgtcacctc acctcgggaa
     1861 atgagaagac ggctcggagg aaccaaaact acttggacat cctgcggctg ctctcgctgg
     1921 gcgaccggca gctcaatgcc tttgacatct ctctgcgttt cacacacctc ttctggtttg
     1981 gggacctcaa ctaccgcctg gacatggata tccaggagat cctgaactac atcagcagga
     2041 aagagtttga gcccctcctc agggtggacc agctcaacct ggagcgggag aagcacaagg
     2101 tcttccttcg attcagtgag gaggagatct ccttcccacc cacctaccgc tatgagcggg
     2161 gttcccggga cacatatgcc tggcacaagc agaagccaac tggggtccgg accaatgtgc
     2221 cctcatggtg tgaccggatt ctgtggaaat cctaccctga aactcacatc atctgcaatt
     2281 cttatggttg cactgatgac atcgtcacca gcgaccattc ccccgtgttt gggacatttg
     2341 aggttggagt tacctcccag ttcatctcca agaaagggct ctcaaagact tcagaccagg
     2401 cctacattga gtttgagagc atcgaggcca ttgtgaagac agccagccgc accaagttct
     2461 tcatcgagtt ctactctacc tgcctggagg aatacaagaa gagctttgag aatgatgccc
     2521 agagcagtga caacatcaac ttcctcaaag tgcagtggtc ttcacgccag ctgcccacgc
     2581 tcaaaccaat tctggctgat atcgagtacc tgcaggacca gcacctcctg ctcacagtca
     2641 agtccatgga tggctatgaa tcctatgggg agtgtgtggt tgcactcaaa tccatgatcg
     2701 gcagcacggc ccaacagttc ctgaccttcc tatcccaccg tggcgaggag acaggcaata
     2761 tcagaggctc catgaaggtg cgggtgccca cggagcgcct gggcacccgt gagcggctct
     2821 acgagtggat cagcattgat aaggatgagg caggagcaaa gagcaaagcc ccctctgtgt
     2881 cccgagggag ccaggagccc aggtcaggga gccgcaagcc agccttcaca gaggcctcct
     2941 gcccgctctc caggttattt gaagaaccag agaaaccgcc accaacgggg aggcccccag
     3001 ccccaccccg agcagctccc cgggaggagc ccttgacccc caggttgaag ccagagggag
     3061 ctcctgaacc agaaggggtg gcggcccccc cacccaagaa cagcttcaat aaccctgcct
     3121 actacgtcct tgaaggggtc ccgcaccagc tgctgccccc ggagccaccc tcgcctgcca
     3181 gggcccctgt cccatctgcc accaagaaca aagtggccat tacagtgcct gctccacagc
     3241 ttgggcacca ccggcaccct cgtgtgggag aggggagttc ttcagatgag gagtctggag
     3301 gcacactgcc ccctccagac tttccacctc caccactgcc ggactcagcc atcttcctgc
     3361 cccccagcct ggatccttta ccagggccag tggtccgggg ccgtggtggg gctgaggccc
     3421 gtggcccacc acctcccaag gcccatccaa ggcctccact gcccccaggc ccctcaccag
     3481 ccagcacttt cctgggggaa gtggccagtg gggatgaccg gtcctgctcg gtgctgcaga
     3541 tggccaagac gctgagcgag gtggactatg cccctgctgg gcctgcacgc tcagcgctcc
     3601 tcccaggccc cctggagctg cagccccccc ggggactgcc ctcggactat ggccggcccc
     3661 tcagcttccc tccaccccgc atccgggaga gcatccagga agacctggca gaggaggctc
     3721 cgtgcctgca gggcgggcgg gccagcgggc tgggcgaggc aggcatgagt gcctggctgc
     3781 gggccatcgg cttggagcgc tatgaggagg gcctggtgca taatggctgg gacgacctgg
     3841 agtttctcag tgacatcacc gaggaggact tggaggaggc tggggtgcag gacccggctc
     3901 acaagcgcct ccttctggac accctgcagc tcagcaagac gcgtacgcgg ccgctcgagg
     3961 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc
     4021 atcaccattg agctagcgtt taaacggccg gccgcggtca tagctgtttc ctgaacagat
     4081 cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     4141 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     4201 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     4261 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     4321 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     4381 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     4441 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     4501 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     4561 ctgattttaa aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc
     4621 catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg
     4681 tccactcagt agatgcctgt tgaattgggt acgcggccag cggcgagcgg tatcagctca
     4741 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4801 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4861 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4921 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4981 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5041 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5101 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5161 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5221 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5281 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5341 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5401 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5461 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5521 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5581 ctaaagtata tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc
     5641 gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc
     5701 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
     5761 cccgcgttta tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt
     5821 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg
     5881 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
     5941 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     6001 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
     6061 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     6121 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     6181 gctatgtcct gatagcgatc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     6241 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     6301 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     6361 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     6421 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     6481 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     6541 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     6601 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     6661 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     6721 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     6781 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     6841 atcatgcgaa acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc
     6901 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     6961 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
     7021 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct
     7081 ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt
     7141 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
     7201 ctgacacaca ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat
     7261 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7321 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     7381 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7441 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7501 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7561 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7621 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7681 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7741 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
//
