LOCUS       sequence-426079-        5765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human SMU1 fragment K170 to P513. N-terminal 6X
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_SMU1:170-513
FEATURES             Location/Qualifiers
     source          1..5765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1052..1186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1089..1108)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1143..1162
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1215..1380
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1564..2018
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1651..1670)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1860..1881
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2044..2148
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2149..2217,2218..3009)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2367..2386)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3180..3768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3669..3688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3922..3939
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4073..4297
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4364..4897)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5067..5072,5073..5089,5090..5095))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5447..5538
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5463..5480
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5597..5614
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5627..5647
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttaagtttgg tcagaaatca catgtggagt gtgctcgatt ttctccagat ggtcagtatt
       61 tggtcactgg gtctgttgat ggattcattg aagtatggaa ctttactact ggaaaaatca
      121 gaaaggatct taagtaccag gcccaagata actttatgat gatggatgat gctgtcctct
      181 gcatgtgttt cagcagagat acagaaatgt tagcaactgg ggcccaagat ggaaaaatca
      241 aggtgtggaa gattcagagt ggacaatgtt taaggagatt tgagagggca cacagtaagg
      301 gtgtcacctg tctaagcttt tctaaggata gcagtcagat ccttagtgct tcttttgacc
      361 agacaattag aattcatggt ttaaaatctg ggaaaaccct gaaggaattt cgtggccatt
      421 cctcctttgt taacgaagca acatttacac aagatggaca ttacattatt agtgcatcct
      481 ctgatggcac tgtaaagatc tggaatatga agaccacaga atgttcaaat acctttaaat
      541 ccctgggcag caccgcaggg acagatatta ccgtcaacag tgtgattcta cttcctaaaa
      601 accctgagca ctttgtggtg tgcaacagat caaacacggt ggtcatcatg aacatgcagg
      661 ggcagattgt cagaagcttc agttctggta aaagagaagg tggggacttt gtttgctgtg
      721 ccctctctcc ccgtggtgaa tggatctact gtgtagggga ggactttgtg ctctactgtt
      781 tcagtacagt cactggcaaa ctggagagaa ctttgacagt gcacgagaag gatgtgattg
      841 gtattgcaca tcaccctcat cagaacctga ttgctaccta cagtgaagat ggactcctaa
      901 agctctggaa accatgatga cgaagcttgt cgagaagtac tagaggatca taatcagcca
      961 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     1021 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     1081 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     1141 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgatatc
     1201 gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa
     1261 ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa
     1321 ataatcctta aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca
     1381 gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact ccatgtgaca
     1441 aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct gtcatctctt
     1501 cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg aatggcgaat
     1561 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1621 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1681 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1741 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     1801 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag
     1861 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     1921 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     1981 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     2041 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2101 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2161 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2221 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2281 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2341 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2401 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2461 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2521 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2581 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2641 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2701 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2761 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2821 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2881 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2941 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3001 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3061 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3121 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3181 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3241 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3301 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3361 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3421 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3481 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3541 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3601 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3661 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3721 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3781 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3841 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3901 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     3961 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca gccgcgtaac
     4021 ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg
     4081 gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact
     4141 agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact
     4201 tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa
     4261 gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac aatttaccga
     4321 acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     4381 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     4441 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     4501 catgcttgag cagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     4561 ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag
     4621 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     4681 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     4741 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     4801 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     4861 tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     4921 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     4981 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     5041 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     5101 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     5161 tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc
     5221 aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgccctggc
     5281 ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg
     5341 aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta
     5401 gctatagttc tagtggttgg ctacgtatac tccggaatat taatagatca tggagataat
     5461 taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta
     5521 ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg gcgcggatct
     5581 cggtccgaaa accatgcatc atcatcatca tcacagcagc ggcagagaaa acttgtattt
     5641 ccagggcaag tggcagcagc atcagggatt gcttcctcct ggtatgacca tagatttgtt
     5701 tcgaggcaag gcagctgtca aagatgtgga agaagaaaag tttcctacac aactgagcag
     5761 gcata
//
