LOCUS       sequence-426080-        8099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human DDB1. Untagged..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-LIC_DDB1:1-1140
FEATURES             Location/Qualifiers
     source          1..8099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        216..307
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     232..249
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    3920..4054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3957..3976)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4011..4030
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4083..4248
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4432..4886
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4519..4538)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4728..4749
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4912..5016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5017..5085,5086..5877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5235..5254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6048..6636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6537..6556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6790..6807
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6941..7165
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(7232..7765)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7935..7940,7941..7957,7958..7963))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc
       61 ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc
      121 atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct
      181 agtggttggc tacgtatact ccggaatatt aatagatcat ggagataatt aaaatgataa
      241 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
      301 tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatctc ggtccgaaaa
      361 ccatgtcgta caactacgtg gtaacggccc agaagcccac cgccgtgaac ggctgcgtga
      421 ccggacactt tacttcggcc gaagacttaa acctgttgat tgccaaaaac acgagattag
      481 agatctatgt ggtcaccgcc gaggggcttc ggcccgtcaa agaggtgggc atgtatggga
      541 agattgcggt catggagctt ttcaggccca agggggagag caaggacctg ctgtttatct
      601 tgacagcgaa gtacaatgcc tgcatcctgg agtataaaca gagtggcgag agcattgaca
      661 tcattacgcg agcccatggc aatgtccagg accgcattgg ccgcccctca gagaccggca
      721 ttattggcat cattgaccct gagtgccgga tgattggcct gcgtctctat gatggccttt
      781 tcaaggttat tccactagat cgcgataata aagaactcaa ggccttcaac atccgcctgg
      841 aggagctgca tgtcattgat gtcaagttcc tatatggttg ccaagcacct actatttgct
      901 ttgtctacca ggaccctcag gggcggcacg taaaaaccta tgaggtgtct ctccgagaaa
      961 aggaattcaa taagggccct tggaaacagg aaaatgtcga agctgaagct tccatggtga
     1021 tcgcagtccc agagcccttt gggggggcca tcatcattgg acaggagtca atcacctatc
     1081 acaatggtga caaatacctg gctattgccc ctcctatcat caagcaaagc acgattgtgt
     1141 gccacaatcg agtggaccct aatggctcaa gatacctgct gggagacatg gaaggccggc
     1201 tcttcatgct gcttttggag aaggaggaac agatggatgg caccgtcact ctcaaggatc
     1261 tccgtgtaga actccttgga gagacctcta ttgctgagtg cttgacatac cttgataatg
     1321 gtgttgtgtt tgtcgggtct cgcctgggtg actcccagct tgtgaagctc aacgttgaca
     1381 gtaatgaaca aggctcctat gtagtggcca tggaaacctt taccaactta ggacccattg
     1441 tcgatatgtg cgtggtggac ctggagaggc aggggcaggg gcagctggtc acttgctctg
     1501 gggctttcaa ggaaggttct ttgcggatca tccggaatgg aattggaatc cacgagcatg
     1561 ccagcattga cttaccaggc atcaaaggat tatggccact gcggtctgac cctaatcgtg
     1621 agactgatga cactttggtg ctctcttttg tgggccagac aagagttctc atgttaaatg
     1681 gagaggaggt agaagaaacc gaactgatgg gtttcgtgga tgatcagcag actttcttct
     1741 gtggcaacgt ggctcatcag cagcttatcc agatcacttc agcatcggtg aggttggtct
     1801 ctcaagaacc caaagctctg gtcagtgaat ggaaggagcc tcaggccaag aacatcagtg
     1861 tggcctcctg caatagcagc caggtggtgg tggctgtagg cagggccctc tactatctgc
     1921 agatccatcc tcaggagctc cggcagatca gccacacaga gatggaacat gaagtggctt
     1981 gcttggacat caccccatta ggagacagca atggactgtc ccctctttgt gccattggcc
     2041 tctggacgga catctcggct cgtatcttga agttgccctc ttttgaacta ctgcacaagg
     2101 agatgctggg tggagagatc attcctcgct ccatcctgat gaccaccttt gagagtagcc
     2161 attacctcct ttgtgccttg ggagatggag cgcttttcta ctttgggctc aacattgaga
     2221 caggtctgtt gagcgaccgt aagaaggtga ctttgggcac ccagcccacc gtattgagga
     2281 cttttcgttc tctttctacc accaacgtct ttgcttgttc tgaccgcccc actgtcatct
     2341 atagcagcaa ccacaaattg gtcttctcaa atgtcaacct caaggaagtg aactacatgt
     2401 gtcccctcaa ttcagatggc tatcctgaca gcctggcgct ggccaacaat agcaccctca
     2461 ccattggcac catcgatgag atccagaagc tgcacattcg cacagttccc ctctatgagt
     2521 ctccaaggaa gatctgctac caggaagtgt cccagtgttt cggggtcctc tccagccgca
     2581 ttgaagtcca agacacgagt gggggcacga cagccttgag gcccagcgct agcacccagg
     2641 ctctgtccag cagtgtaagc tccagcaagc tgttctccag cagcactgct cctcatgaga
     2701 cctcctttgg agaagaggtg gaggtgcaca acctacttat cattgaccaa cacacctttg
     2761 aagtgcttca tgcccaccag tttctgcaga atgaatatgc cctcagtctg gtttcctgca
     2821 agctgggcaa agaccccaac acttacttca ttgtgggcac agcaatggtg tatcctgaag
     2881 aggcagagcc caagcagggt cgcattgtgg tctttcagta ttcggatgga aaactacaga
     2941 ctgtggctga aaaggaagtg aaaggggccg tgtactctat ggtggaattt aacgggaagc
     3001 tgttagccag catcaatagc acggtgcggc tctatgagtg gacaacagag aaggagctgc
     3061 gcactgagtg caaccactac aacaacatca tggccctcta cctgaagacc aagggcgact
     3121 tcatcctggt gggcgacctt atgcgctcag tgctgctgct tgcctacaag cccatggaag
     3181 gaaactttga agagattgct cgagacttta atcccaactg gatgagtgct gtggaaatct
     3241 tggatgatga caattttctg ggggctgaaa atgcctttaa cttgtttgtg tgtcaaaagg
     3301 atagcgctgc caccactgac gaggagcggc agcacctcca ggaggttggt cttttccacc
     3361 tgggcgagtt tgtcaatgtc ttttgccacg gctctctggt aatgcagaat ctgggtgaga
     3421 cttccacccc cacacaaggc tcggtgctct tcggcacggt caacggcatg atagggctgg
     3481 tgacctcact gtcagagagc tggtacaacc tcctgctgga catgcagaat cgactcaata
     3541 aagtcatcaa aagtgtgggg aagatcgagc actccttctg gagatccttt cacaccgagc
     3601 ggaagacaga accagccaca ggtttcatcg acggtgactt gattgagagt ttcctggata
     3661 ttagccgccc caagatgcag gaggtggtgg caaacctaca gtatgacgat ggcagcggta
     3721 tgaagcgaga ggccactgca gacgacctca tcaaggttgt ggaggagcta actcggatcc
     3781 attgatgacg aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt
     3841 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     3901 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3961 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4021 caaactcatc aatgtatctt atcatgtctg gatctgatca ctgatatcgc ctaggagatc
     4081 cgaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag
     4141 cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa
     4201 aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt
     4261 tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt
     4321 cggctacttt ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt
     4381 ttgtaattga ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct
     4441 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4501 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4561 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4621 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4681 gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt
     4741 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4801 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4861 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     4921 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4981 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5041 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5101 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5161 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5221 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5281 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5341 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5401 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5461 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5521 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5581 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5641 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5701 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5761 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5821 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5881 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5941 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6001 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6061 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6121 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6181 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6241 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6301 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6361 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6421 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6481 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6541 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6601 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6661 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6721 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6781 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     6841 cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg
     6901 gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc
     6961 ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt
     7021 tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct
     7081 aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc
     7141 caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg
     7201 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
     7261 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
     7321 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagca
     7381 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
     7441 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
     7501 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
     7561 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
     7621 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
     7681 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
     7741 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     7801 tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca
     7861 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     7921 gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca
     7981 tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc
     8041 tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcg
//
