LOCUS       sequence-426084-        5684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human RACK1. N-terminal 6X His tag 
            with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_RACK1:1-317
FEATURES             Location/Qualifiers
     source          1..5684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  49..273
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(340..873)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1043..1048,1049..1065,1066..1071))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1423..1514
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1439..1456
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1573..1590
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1603..1623
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2712..2846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2749..2768)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2803..2822
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2875..3040
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3224..3678
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3311..3330)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3520..3541
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3704..3808
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3809..3877,3878..4669)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4027..4046)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4840..5428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5329..5348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5582..5599
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaatcggt tacggttgag taataaatgg atgccctgcg taagcgggtg tgggcggaca
       61 ataaagtctt aaactgaaca aaatagatct aaactatgac aataaagtct taaactagac
      121 agaatagttg taaactgaaa tcagtccagt tatgctgtga aaaagcatac tggacttttg
      181 ttatggctaa agcaaactct tcattttctg aagtgcaaat tgcccgtcgt attaaagagg
      241 ggcgtggcca agggcatggt aaagactata ttcgcggcgt tgtgacaatt taccgaacaa
      301 ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc
      361 gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg
      421 atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg
      481 cttgagcaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc
      541 gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc
      601 gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg
      661 gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc
      721 gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag
      781 cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc
      841 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
      901 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
      961 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     1021 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     1081 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga
     1141 ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc
     1201 attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc cctggcttca
     1261 ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt
     1321 ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg actctagcta
     1381 tagttctagt ggttggctac gtatactccg gaatattaat agatcatgga gataattaaa
     1441 atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa
     1501 aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc ggatctcggt
     1561 ccgaaaacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
     1621 ggcatgactg agcagatgac ccttcgtggc accctcaagg gccacaacgg ctgggtaacc
     1681 cagatcgcta ctaccccgca gttcccggac atgatcctct ccgcctctcg agataagacc
     1741 atcatcatgt ggaaactgac cagggatgag accaactatg gaattccaca gcgtgctctg
     1801 cggggtcact cccactttgt tagtgatgtg gttatctcct cagatggcca gtttgccctc
     1861 tcaggctcct gggatggaac cctgcgcctc tgggatctca caacgggcac caccacgagg
     1921 cgatttgtgg gccataccaa ggatgtgctg agtgtggcct tctcctctga caaccggcag
     1981 attgtctctg gatctcgaga taaaaccatc aagctatgga ataccctggg tgtgtgcaaa
     2041 tacactgtcc aggatgagag ccactcagag tgggtgtctt gtgtccgctt ctcgcccaac
     2101 agcagcaacc ctatcatcgt ctcctgtggc tgggacaagc tggtcaaggt atggaacctg
     2161 gctaactgca agctgaagac caaccacatt ggccacacag gctatctgaa cacggtgact
     2221 gtctctccag atggatccct ctgtgcttct ggaggcaagg atggccaggc catgttatgg
     2281 gatctcaacg aaggcaaaca cctttacacg ctagatggtg gggacatcat caacgccctg
     2341 tgcttcagcc ctaaccgcta ctggctgtgt gctgccacag gccccagcat caagatctgg
     2401 gatttagagg gaaagatcat tgtagatgaa ctgaagcaag aagttatcag taccagcagc
     2461 aaggcagaac caccccagtg cacctccctg gcctggtctg ctgatggcca gactctgttt
     2521 gctggctaca cggacaacct ggtgcgagtg tggcaggtga ccattggcac acgctgatga
     2581 cgaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt
     2641 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     2701 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2761 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2821 tcaatgtatc ttatcatgtc tggatctgat cactgatatc gcctaggaga tccgaaccag
     2881 ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac
     2941 gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat
     3001 ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg
     3061 ttatgttttt aatcaaacat cctgccaact ccatgtgaca aaccgtcatc ttcggctact
     3121 ttttctctgt cacagaatga aaatttttct gtcatctctt cgttattaat gtttgtaatt
     3181 gactgaatat caacgcttat ttgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     3241 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3301 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3361 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3421 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3481 gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag tggactcttg ttccaaactg
     3541 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3601 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3661 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3721 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3781 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3841 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3901 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3961 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4021 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4081 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4141 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4201 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4261 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4321 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4381 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4441 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4501 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4561 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4621 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4681 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4741 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4801 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4861 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4921 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4981 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5041 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5101 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5161 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5221 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5281 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5341 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5401 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5461 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5521 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5581 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     5641 tctgtgcggt atttcacacc gcatagacca gccgcgtaac ctgg
//
