LOCUS       sequence-426085-        6129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of the truncated variants of the SHIP2 that retains only 
            SH2 domain and first proline-rich domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Fafilek B, Balek L, Bosakova MK, Varecha M, Nita A, Gregor T, 
            Gudernova I, Krenova J, Ghosh S, Piskacek M, Jonatova L, Cernohorsky
            NH, Zieba JT, Kostas M, Haugsten EM, Wesche J, Erneux C, Trantirek 
            L, Krakow D, Krejci P
  TITLE     The inositol phosphatase SHIP2 enables sustained ERK activation 
            downstream of FGF receptors by recruiting Src kinases.
  JOURNAL   Sci Signal. 2018 Sep 18;11(548):eaap8608. doi: 
            10.1126/scisignal.aap8608.
  PUBMED    30228226
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2018-09-18"; volume: "11(548):eaap8608. doi"; 
            pages: " 10.1126/scisignal.aap8608"
COMMENT     Sequence Label: SHIP2-N1
FEATURES             Location/Qualifiers
     source          1..6129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2289..2330
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2340..2357
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2386..2402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2386..2402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2413..3035
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2612..2631)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(3184..3772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3264..3283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4101..4148
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4173..4192
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4380..5174)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4380..5174)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4491..4510)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5101..5120
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5209..5566)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5223..5358
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5375..5566
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5523..5543
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(5568..5672)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5699..6127
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5786..5805)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5996..6017
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcctcggcc tgcggggcgc cgggcccggg gggcgccctg ggcagccagg
     1081 ccccctcctg gtaccaccgc gacctgagcc gggcggccgc ggaggagctg ctggcccggg
     1141 cgggccgcga tggcagcttc ctggtccgag acagcgagag cgtggcgggg gccttcgcgc
     1201 tctgcgtcct gtatcagaag catgtgcaca cgtatcgcat tctgcctgat ggagaagatt
     1261 tcttggctgt gcagacctcg cagggtgtgc ctgtgcgccg cttccagacc ctgggtgagc
     1321 tcatcggcct gtacgcccag cccaaccagg gccttgtgtg cgccctgctt cttcctgtag
     1381 agggtgagcg agagccggac ccaccggatg accgggatgc ctcagatggg gaggatgaga
     1441 agcccccgct gcccccgcgc tctggctcca ccagcatttc tgcccccact gggcccagca
     1501 gtcccctgcc agctcctgag actcccacag ctccagctgc tgagagtgct cccaatgggc
     1561 tgagcaccgt ctcgcacgac tacctgaaag gcagctatgg gctggacctg gaagctgtga
     1621 ggggtggagc cagccacctg ccccacctca cccgtaccct cgctacctca tgccggaggc
     1681 tgcacagtga ggtggacaag gtcctgtcag gcctggagat cctgtccaag gtgtttgacc
     1741 agcagagctc gcccatggtg acccgccttt tgcagcagca gaacctgcca cagacagggg
     1801 agcaggaact agagagcctg gtgctgaagc tgtcagtgct aaaggacttc ctgtcaggca
     1861 tccagaagaa ggccctgaag gccctacagg acatgagctc cacagcaccc ccagctccgc
     1921 agccatccac acgtaaggcc aagaccatcc ccgtgcaggc ctttgaggtg aagctagatg
     1981 tgaccctggg tgacctgacc aagattggga agtcacagaa gttcacgctg agcgtggatg
     2041 tggagggtgg gcggctggtg ctgctgcgga gacagcggga ctcccaggag gactggacca
     2101 ccttcacgca cgaccgcatc cgccagctca ttaagtccca gcgtgtccag aacaagctgg
     2161 gtgttgtgtt tgagaaggag aaggaccgga ctcagcgcaa ggacttcatc tttgtcagtg
     2221 cccggaagcg ggaggccttc tgccagctgt tgcagctcat gaagaacaag cactccaagc
     2281 agctcgaggg taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc
     2341 atcatcacca tcaccattga gctagcgttt aaacggccgg ccgcggtcat agctgtttcc
     2401 tgaacagatc ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
     2461 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
     2521 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
     2581 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
     2641 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
     2701 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
     2761 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
     2821 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
     2881 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg
     2941 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca
     3001 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ggcgagcggt
     3061 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3601 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3901 taaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac
     3961 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga
     4021 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4081 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt
     4141 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4201 ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
     4261 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa
     4321 atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     4381 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     4441 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     4501 gtagccaacg ctatgtcctg atagcgatcc gccacaccca gccggccaca gtcgatgaat
     4561 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     4621 acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg
     4681 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     4741 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     4801 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     4861 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     4921 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     4981 gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     5041 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     5101 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     5161 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc
     5221 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     5281 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     5341 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     5401 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     5461 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     5521 acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact cttccttttt
     5581 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5641 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     5701 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5761 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5821 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5881 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5941 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6001 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6061 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6121 gcgaatttt
//
