LOCUS       sequence-426086-        6023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human WDR41 fragment Q30 to V459. N-terminal 6X
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_WDR41_30-459
FEATURES             Location/Qualifiers
     source          1..6023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    995..1129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1032..1051)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1086..1105
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1158..1323
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1507..1961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1594..1613)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1803..1824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1987..2091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2092..2160,2161..2952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2310..2329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3123..3711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3612..3631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3865..3882
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4016..4240
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4307..4840)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5010..5015,5016..5032,5033..5038))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5390..5481
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5406..5423
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5540..5557
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5570..5590
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 caggtattag tgctttggtt gaaataccta agaactgtgt tgtggcagca gttggcaaag
       61 aactgataat tttcaggttg gtagcaccca cagaaggatc actagaatgg gatattcttg
      121 aagttaagcg cctccttgat caccaggata atattctctc attgattaat gtcaatgatt
      181 tgagttttgt caccggctcc cacgtcggag agctgatcat ctgggatgcc ctggactgga
      241 ccatgcaggc ctatgaacgc aacttctggg acccatctcc acaactggac acccaacaag
      301 aaataaaact ctgtcaaaaa tcaaatgaca tttctattca tcatttcaca tgtgatgaag
      361 agaatgtatt tgctgcagtt ggaaggggtt tatacgtgta tagccttcaa atgaagcgtg
      421 tgattgcctg ccagaaaact gcacatgact ccaatgtcct gcacgttgcc agacttccaa
      481 acaggcagtt aatctcatgc tcagaagatg gcagtgtacg catttgggag ttaagagaaa
      541 aacagcagct tgcagctgag cctgtaccaa caggtttttt taacatgtgg ggatttggaa
      601 gagtcagcaa acaagccagc caacctgtta aaaagcagca agaaaatgct acttcatgtt
      661 cactggagct tattggagat ttgattggac actcatcatc tgtggagatg tttctatact
      721 ttgaagatca tggactagtg acgtgctccg ctgatcatct cattattttg tggaaaaatg
      781 gagagcgaga atctggattg cgcagtttaa gattatttca aaaattagag gagaatggtg
      841 acttatacct tgctgtctga tgacgaagct tgtcgagaag tactagagga tcataatcag
      901 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      961 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     1021 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     1081 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgat
     1141 atcgcctagg agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     1201 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     1261 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     1321 acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg
     1381 acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct
     1441 cttcgttatt aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg
     1501 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1561 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     1621 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     1681 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     1741 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttaa
     1801 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     1861 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     1921 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     1981 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2041 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2101 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2161 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2221 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2281 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2341 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2401 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2461 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2521 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2581 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2641 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2701 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2761 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2821 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2881 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2941 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3001 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3061 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3121 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3181 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3241 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3301 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3361 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3421 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3481 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3541 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3661 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3721 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3781 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3841 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     3901 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga ccagccgcgt
     3961 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg
     4021 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa
     4081 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg
     4141 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt
     4201 aaagaggggc gtggccaagg gcatggtaaa gactatattc gcggcgttgt gacaatttac
     4261 cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac
     4321 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
     4381 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
     4441 cctcatgctt gagcagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg
     4501 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt
     4561 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct
     4621 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
     4681 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
     4741 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
     4801 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     4861 aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag tcataacaag
     4921 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
     4981 tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact
     5041 gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg
     5101 aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc tccacgcatc
     5161 gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg gatctgccct
     5221 ggcttcagga gatcggaaga cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg
     5281 atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc gcccaggact
     5341 ctagctatag ttctagtggt tggctacgta tactccggaa tattaataga tcatggagat
     5401 aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt
     5461 gtaataaaaa aacctataaa tattccggat tattcatacc gtcccaccat cgggcgcgga
     5521 tctcggtccg aaaaccatgc atcatcatca tcatcacagc agcggcagag aaaacttgta
     5581 tttccagggc cagaatccct acactgaact gctagtactg aaggctcatc atgatattgt
     5641 acgatttctg gtacagttag atgactacag atttgcatct gctggtgatg atggaattgt
     5701 agttgtgtgg aatgcccaga caggggaaaa acttttagaa ctgaatggac acactcaaaa
     5761 gataacagct attattacat ttccttcctt ggaatcttgt gaagagaaaa atcaactcat
     5821 cttgacagcc tctgctgata gaacagttat tgtgtgggat ggtgatacta ccagacaagt
     5881 tcagagaata tcatgcttcc agtctactgt aaagtgttta actgttcttc agagactaga
     5941 tgtttggctt tctggtggga atgacctgtg tgtgtggaac cgaaaattag atctcctgtg
     6001 taagactagc cacctttctg ata
//
