LOCUS       sequence-426087-        5966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human WDR41 fragment Q30 to L441. N-terminal 6X
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_WDR41_30-441
FEATURES             Location/Qualifiers
     source          1..5966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1153..1287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1190..1209)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1244..1263
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1316..1481
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1665..2119
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1752..1771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1961..1982
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2145..2249
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2250..2318,2319..3110)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2468..2487)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3281..3869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3770..3789
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4023..4040
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4174..4398
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4465..4998)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5168..5173,5174..5190,5191..5196))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5548..5639
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5564..5581
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5698..5715
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5728..5748
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aaatcaactc atcttgacag cctctgctga tagaacagtt attgtgtggg atggtgatac
       61 taccagacaa gttcagagaa tatcatgctt ccagtctact gtaaagtgtt taactgttct
      121 tcagagacta gatgtttggc tttctggtgg gaatgacctg tgtgtgtgga accgaaaatt
      181 agatctcctg tgtaagacta gccacctttc tgatacaggt attagtgctt tggttgaaat
      241 acctaagaac tgtgttgtgg cagcagttgg caaagaactg ataattttca ggttggtagc
      301 acccacagaa ggatcactag aatgggatat tcttgaagtt aagcgcctcc ttgatcacca
      361 ggataatatt ctctcattga ttaatgtcaa tgatttgagt tttgtcaccg gctcccacgt
      421 cggagagctg atcatctggg atgccctgga ctggaccatg caggcctatg aacgcaactt
      481 ctgggaccca tctccacaac tggacaccca acaagaaata aaactctgtc aaaaatcaaa
      541 tgacatttct attcatcatt tcacatgtga tgaagagaat gtatttgctg cagttggaag
      601 gggtttatac gtgtatagcc ttcaaatgaa gcgtgtgatt gcctgccaga aaactgcaca
      661 tgactccaat gtcctgcacg ttgccagact tccaaacagg cagttaatct catgctcaga
      721 agatggcagt gtacgcattt gggagttaag agaaaaacag cagcttgcag ctgagcctgt
      781 accaacaggt ttttttaaca tgtggggatt tggaagagtc agcaaacaag ccagccaacc
      841 tgttaaaaag cagcaagaaa atgctacttc atgttcactg gagcttattg gagatttgat
      901 tggacactca tcatctgtgg agatgtttct atactttgaa gatcatggac tagtgacgtg
      961 ctccgctgat catctcatta ttttgtggaa aaatggagag cgagaatctg gattgtgatg
     1021 acgaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt
     1081 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     1141 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1201 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1261 atcaatgtat cttatcatgt ctggatctga tcactgatat cgcctaggag atccgaacca
     1321 gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga
     1381 cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca
     1441 tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat ttttcttcct
     1501 gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat cttcggctac
     1561 tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa tgtttgtaat
     1621 tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     1681 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     1741 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     1801 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     1861 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     1921 ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt gttccaaact
     1981 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     2041 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     2101 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2161 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2221 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2281 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2341 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2401 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2461 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2521 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2581 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2641 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2701 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2761 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2821 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2881 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2941 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3001 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3061 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3121 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3181 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3241 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3301 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3361 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3421 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3481 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3541 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3601 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3661 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3721 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3781 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3841 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3901 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3961 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4021 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     4081 atctgtgcgg tatttcacac cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg
     4141 ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact
     4201 gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac
     4261 tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa
     4321 actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc
     4381 atggtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa
     4441 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
     4501 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
     4561 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga gcagattgat
     4621 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
     4681 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
     4741 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
     4801 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
     4861 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
     4921 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     4981 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     5041 ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     5101 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
     5161 gcattacagt ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt
     5221 ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg
     5281 gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt caggcattgg cggccttgct
     5341 gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg cttcaggaga tcggaagacc
     5401 tcggccgtcg cggcgcttgc cggtggtgct gaccccggat gaagtggttc gcatcctcgg
     5461 ttttctggaa ggcgagcatc gtttgttcgc ccaggactct agctatagtt ctagtggttg
     5521 gctacgtata ctccggaata ttaatagatc atggagataa ttaaaatgat aaccatctcg
     5581 caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata
     5641 ttccggatta ttcataccgt cccaccatcg ggcgcggatc tcggtccgaa aaccatgcat
     5701 catcatcatc atcacagcag cggcagagaa aacttgtatt tccagggcca gaatccctac
     5761 actgaactgc tagtactgaa ggctcatcat gatattgtac gatttctggt acagttagat
     5821 gactacagat ttgcatctgc tggtgatgat ggaattgtag ttgtgtggaa tgcccagaca
     5881 ggggaaaaac ttttagaact gaatggacac actcaaaaga taacagctat tattacattt
     5941 ccttccttgg aatcttgtga agagaa
//
