LOCUS       sequence-426088-        7217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human DDB1 with a loop deletion 
            I396 to D689. Untagged..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-LIC_DDB1:1-1140_delta:396-689
FEATURES             Location/Qualifiers
     source          1..7217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(71..90)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     280..301
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        464..568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(569..637,638..1429)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(787..806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1600..2188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2089..2108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2342..2359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2493..2717
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2784..3317)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3487..3492,3493..3509,3510..3515))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3867..3958
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3883..3900
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    6689..6823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6726..6745)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6780..6799
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6852..7017
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      join(7201..7217,1..438)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
       61 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      121 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      181 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      241 gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag tggactcttg ttccaaactg
      301 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
      361 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      421 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      481 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      541 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      601 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      661 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      721 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      781 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      841 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      901 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      961 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1021 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1081 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1141 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1201 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1261 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1321 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1381 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1441 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1501 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1561 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1621 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1681 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1741 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1801 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1861 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1921 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1981 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2041 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2101 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2161 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2221 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2281 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2341 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2401 tctgtgcggt atttcacacc gcatagacca gccgcgtaac ctggcaaaat cggttacggt
     2461 tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg
     2521 aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact
     2581 gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa
     2641 ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gccaagggca
     2701 tggtaaagac tatattcgcg gcgttgtgac aatttaccga acaactccgc ggccgggaag
     2761 ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc
     2821 acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct
     2881 gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag cagattgatg
     2941 agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag
     3001 atctcactac gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc
     3061 gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg
     3121 taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa
     3181 agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg
     3241 atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     3301 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     3361 cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     3421 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
     3481 cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt catccgtttc
     3541 cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg
     3601 ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc ggccttgctg
     3661 ttcttctacg gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct
     3721 cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt
     3781 tttctggaag gcgagcatcg tttgttcgcc caggactcta gctatagttc tagtggttgg
     3841 ctacgtatac tccggaatat taatagatca tggagataat taaaatgata accatctcgc
     3901 aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat
     3961 tccggattat tcataccgtc ccaccatcgg gcgcggatct cggtccgaaa accatgtcgt
     4021 acaactacgt ggtaacggcc cagaagccca ccgccgtgaa cggctgcgtg accggacact
     4081 ttacttcggc cgaagactta aacctgttga ttgccaaaaa cacgagatta gagatctatg
     4141 tggtcaccgc cgaggggctt cggcccgtca aagaggtggg catgtatggg aagattgcgg
     4201 tcatggagct tttcaggccc aagggggaga gcaaggacct gctgtttatc ttgacagcga
     4261 agtacaatgc ctgcatcctg gagtataaac agagtggcga gagcattgac atcattacgc
     4321 gagcccatgg caatgtccag gaccgcattg gccgcccctc agagaccggc attattggca
     4381 tcattgaccc tgagtgccgg atgattggcc tgcgtctcta tgatggcctt ttcaaggtta
     4441 ttccactaga tcgcgataat aaagaactca aggccttcaa catccgcctg gaggagctgc
     4501 atgtcattga tgtcaagttc ctatatggtt gccaagcacc tactatttgc tttgtctacc
     4561 aggaccctca ggggcggcac gtaaaaacct atgaggtgtc tctccgagaa aaggaattca
     4621 ataagggccc ttggaaacag gaaaatgtcg aagctgaagc ttccatggtg atcgcagtcc
     4681 cagagccctt tgggggggcc atcatcattg gacaggagtc aatcacctat cacaatggtg
     4741 acaaatacct ggctattgcc cctcctatca tcaagcaaag cacgattgtg tgccacaatc
     4801 gagtggaccc taatggctca agatacctgc tgggagacat ggaaggccgg ctcttcatgc
     4861 tgcttttgga gaaggaggaa cagatggatg gcaccgtcac tctcaaggat ctccgtgtag
     4921 aactccttgg agagacctct attgctgagt gcttgacata ccttgataat ggtgttgtgt
     4981 ttgtcgggtc tcgcctgggt gactcccagc ttgtgaagct caacgttgac agtaatgaac
     5041 aaggctccta tgtagtggcc atggaaacct ttaccaactt aggacccatt gtcgatatgt
     5101 gcgtggtgga cctggagagg caggggcagg ggcagctggt cacttgctct ggggctttca
     5161 aggaaggttc tttgcggatc atccggaatg gaattggaag cctggcgctg gccaacaata
     5221 gcaccctcac cattggcacc atcgatgaga tccagaagct gcacattcgc acagttcccc
     5281 tctatgagtc tccaaggaag atctgctacc aggaagtgtc ccagtgtttc ggggtcctct
     5341 ccagccgcat tgaagtccaa gacacgagtg ggggcacgac agccttgagg cccagcgcta
     5401 gcacccaggc tctgtccagc agtgtaagct ccagcaagct gttctccagc agcactgctc
     5461 ctcatgagac ctcctttgga gaagaggtgg aggtgcacaa cctacttatc attgaccaac
     5521 acacctttga agtgcttcat gcccaccagt ttctgcagaa tgaatatgcc ctcagtctgg
     5581 tttcctgcaa gctgggcaaa gaccccaaca cttacttcat tgtgggcaca gcaatggtgt
     5641 atcctgaaga ggcagagccc aagcagggtc gcattgtggt ctttcagtat tcggatggaa
     5701 aactacagac tgtggctgaa aaggaagtga aaggggccgt gtactctatg gtggaattta
     5761 acgggaagct gttagccagc atcaatagca cggtgcggct ctatgagtgg acaacagaga
     5821 aggagctgcg cactgagtgc aaccactaca acaacatcat ggccctctac ctgaagacca
     5881 agggcgactt catcctggtg ggcgacctta tgcgctcagt gctgctgctt gcctacaagc
     5941 ccatggaagg aaactttgaa gagattgctc gagactttaa tcccaactgg atgagtgctg
     6001 tggaaatctt ggatgatgac aattttctgg gggctgaaaa tgcctttaac ttgtttgtgt
     6061 gtcaaaagga tagcgctgcc accactgacg aggagcggca gcacctccag gaggttggtc
     6121 ttttccacct gggcgagttt gtcaatgtct tttgccacgg ctctctggta atgcagaatc
     6181 tgggtgagac ttccaccccc acacaaggct cggtgctctt cggcacggtc aacggcatga
     6241 tagggctggt gacctcactg tcagagagct ggtacaacct cctgctggac atgcagaatc
     6301 gactcaataa agtcatcaaa agtgtgggga agatcgagca ctccttctgg agatcctttc
     6361 acaccgagcg gaagacagaa ccagccacag gtttcatcga cggtgacttg attgagagtt
     6421 tcctggatat tagccgcccc aagatgcagg aggtggtggc aaacctacag tatgacgatg
     6481 gcagcggtat gaagcgagag gccactgcag acgacctcat caaggttgtg gaggagctaa
     6541 ctcggatcca ttgatgacga agcttgtcga gaagtactag aggatcataa tcagccatac
     6601 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     6661 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     6721 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6781 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgatatcgcc
     6841 taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca tttttaattt
     6901 tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata
     6961 atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc gcccacagcg
     7021 gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca tgtgacaaac
     7081 cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc atctcttcgt
     7141 tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat ggcgaatggg
     7201 acgcgccctg tagcggc
//
