LOCUS       sequence-426089-        5966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human RBBP7 fragment M1 to E411. N-terminal 6X 
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_RBBP7:1-411
FEATURES             Location/Qualifiers
     source          1..5966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(199..732)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(902..907,908..924,925..930))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1282..1373
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1298..1315
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1432..1449
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1462..1482
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2853..2987
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2890..2909)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2944..2963
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3016..3181
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3365..3819
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3452..3471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3661..3682
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3845..3949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3950..4018,4019..4810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4168..4187)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4981..5569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5470..5489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5723..5740
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  join(5874..5966,1..132)
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 cagtccagtt atgctgtgaa aaagcatact ggacttttgt tatggctaaa gcaaactctt
       61 cattttctga agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa gggcatggta
      121 aagactatat tcgcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat
      181 ctcggcttga acgaattgtt aggtggcggt acttgggtcg atatcaaagt gcatcacttc
      241 ttcccgtatg cccaactttg tatagagagc cactgcggga tcgtcaccgt aatctgcttg
      301 cacgtagatc acataagcac caagcgcgtt ggcctcatgc ttgagcagat tgatgagcgc
      361 ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg agatcataga tatagatctc
      421 actacgcggc tgctcaaact tgggcagaac gtaagccgcg agagcgccaa caaccgcttc
      481 ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg agcaagttcc cgaggtaatc
      541 ggagtccggc tgatgttggg agtaggtggc tacgtctccg aactcacgac cgaaaagatc
      601 aagagcagcc cgcatggatt tgacttggtc agggccgagc ctacatgtgc gaatgatgcc
      661 catacttgag ccacctaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
      721 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
      781 catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc
      841 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta
      901 cagtttacga accgaacagg cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg
      961 tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc
     1021 gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt
     1081 ctacggcaag gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc
     1141 gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct
     1201 ggaaggcgag catcgtttgt tcgcccagga ctctagctat agttctagtg gttggctacg
     1261 tatactccgg aatattaata gatcatggag ataattaaaa tgataaccat ctcgcaaata
     1321 aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatattccgg
     1381 attattcata ccgtcccacc atcgggcgcg gatctcggtc cgaaaaccat gcatcatcat
     1441 catcatcaca gcagcggcag agaaaacttg tatttccagg gcatggcgag taaagagatg
     1501 tttgaagata ctgtggagga gcgtgtcatc aatgaagaat ataaaatctg gaagaagaat
     1561 acaccgtttc tatatgacct ggttatgacc catgctcttc agtggcccag tcttaccgtt
     1621 cagtggcttc ctgaagtgac taaacctgaa ggaaaagatt atgcccttca ttggctagtg
     1681 ctggggactc atacgtctga tgagcagaat catctggtgg ttgctcgagt acatattccc
     1741 aatgatgatg cacagtttga tgcttcccat tgtgacagtg acaagggtga atttggtggc
     1801 tttggttctg taacaggaaa aattgaatgt gaaattaaaa tcaatcacga aggagaagta
     1861 aaccgtgctc gttacatgcc gcagaatcct cacatcattg ctacaaaaac accatcttct
     1921 gatgtgttgg tttttgacta tacaaaacac cctgctaaac cagacccaag tggagaatgt
     1981 aatcctgatc tcagattaag aggtcaccag aaggaaggct atggtctctc ctggaattca
     2041 aatttgagtg gacatctcct aagtgcatct gatgaccata ctgtttgtct gtgggatata
     2101 aacgcaggac caaaagaagg caaaattgtg gatgctaaag ccatctttac tggccactca
     2161 gctgttgtag aggatgtggc ctggcacctg ctgcacgagt cattgtttgg atctgttgct
     2221 gatgatcaga aacttatgat atgggacacc aggtccaata ccacctccaa gccgagtcac
     2281 ttggtggatg cgcacactgc cgaagtcaac tgcctctcat tcaatcccta cagcgaattt
     2341 attctagcca ccggctctgc ggataagacc gtagctttat gggatctgcg taacttaaaa
     2401 ttaaaactcc ataccttcga atctcataaa gatgaaattt tccaggtcca ctggtctcca
     2461 cataatgaaa ctattctggc ttcaagtggt actgaccgcc gcctgaatgt gtgggattta
     2521 agtaaaattg gggaagaaca atcagcagaa gatgcagaag atgggcctcc agaactcctg
     2581 tttattcatg gaggacacac tgctaagatt tcagatttta gctggaaccc caatgagcct
     2641 tgggtcattt gctcagtgtc tgaggataac atcatgcaga tatggcaaat ggctgaaaat
     2701 atttacaatg atgaatgatg acgaagcttg tcgagaagta ctagaggatc ataatcagcc
     2761 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2821 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2881 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2941 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgatat
     3001 cgcctaggag atccgaacca gataagtgaa atctagttcc aaactatttt gtcattttta
     3061 attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta
     3121 aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac
     3181 agcggggcat ttttcttcct gttatgtttt taatcaaaca tcctgccaac tccatgtgac
     3241 aaaccgtcat cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct
     3301 tcgttattaa tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa
     3361 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3421 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3481 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3541 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3601 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttcttaata
     3661 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3721 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3781 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     3841 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3901 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3961 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4021 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4081 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4141 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4201 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4261 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4321 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4381 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4441 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4501 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4561 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4621 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4681 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4741 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4801 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4861 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4921 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4981 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5041 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5101 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5161 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5221 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5281 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5341 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5401 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5461 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5521 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5581 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5641 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5701 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     5761 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagacc agccgcgtaa
     5821 cctggcaaaa tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc
     5881 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
     5941 tagacagaat agttgtaaac tgaaat
//
