LOCUS       sequence-426096-        5722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of the truncated variants of the SHIP2 that retains first
            proline-rich domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Fafilek B, Balek L, Bosakova MK, Varecha M, Nita A, Gregor T, 
            Gudernova I, Krenova J, Ghosh S, Piskacek M, Jonatova L, Cernohorsky
            NH, Zieba JT, Kostas M, Haugsten EM, Wesche J, Erneux C, Trantirek 
            L, Krakow D, Krejci P
  TITLE     The inositol phosphatase SHIP2 enables sustained ERK activation 
            downstream of FGF receptors by recruiting Src kinases.
  JOURNAL   Sci Signal. 2018 Sep 18;11(548):eaap8608. doi: 
            10.1126/scisignal.aap8608.
  PUBMED    30228226
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2018-09-18"; volume: "11(548):eaap8608. doi"; 
            pages: " 10.1126/scisignal.aap8608"
COMMENT     Sequence Label: SHIP2-N2
FEATURES             Location/Qualifiers
     source          1..5722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1882..1923
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1933..1950
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1979..1995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1979..1995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2006..2628
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2205..2224)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(2777..3365)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2857..2876)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3694..3741
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3766..3785
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3973..4767)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3973..4767)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4084..4103)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4694..4713
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4802..5159)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4816..4951
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4968..5159
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5116..5136
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(5161..5265)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5292..5720
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5379..5398)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5589..5610
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcatgccg gacccaccgg atgaccggga tgcctcagat
     1021 ggggaggatg agaagccccc gctgcccccg cgctctggct ccaccagcat ttctgccccc
     1081 actgggccca gcagtcccct gccagctcct gagactccca cagctccagc tgctgagagt
     1141 gctcccaatg ggctgagcac cgtctcgcac gactacctga aaggcagcta tgggctggac
     1201 ctggaagctg tgaggggtgg agccagccac ctgccccacc tcacccgtac cctcgctacc
     1261 tcatgccgga ggctgcacag tgaggtggac aaggtcctgt caggcctgga gatcctgtcc
     1321 aaggtgtttg accagcagag ctcgcccatg gtgacccgcc ttttgcagca gcagaacctg
     1381 ccacagacag gggagcagga actagagagc ctggtgctga agctgtcagt gctaaaggac
     1441 ttcctgtcag gcatccagaa gaaggccctg aaggccctac aggacatgag ctccacagca
     1501 cccccagctc cgcagccatc cacacgtaag gccaagacca tccccgtgca ggcctttgag
     1561 gtgaagctag atgtgaccct gggtgacctg accaagattg ggaagtcaca gaagttcacg
     1621 ctgagcgtgg atgtggaggg tgggcggctg gtgctgctgc ggagacagcg ggactcccag
     1681 gaggactgga ccaccttcac gcacgaccgc atccgccagc tcattaagtc ccagcgtgtc
     1741 cagaacaagc tgggtgttgt gtttgagaag gagaaggacc ggactcagcg caaggacttc
     1801 atctttgtca gtgcccggaa gcgggaggcc ttctgccagc tgttgcagct catgaagaac
     1861 aagcactcca agcagctcga gggtaagcct atccctaacc ctctcctcgg tctcgattct
     1921 acgcgtaccg gtcatcatca ccatcaccat tgagctagcg tttaaacggc cggccgcggt
     1981 catagctgtt tcctgaacag atcccgggtg gcatccctgt gacccctccc cagtgcctct
     2041 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg
     2101 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg
     2161 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag
     2221 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat
     2281 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta
     2341 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc
     2401 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac
     2461 cactgctccc ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca
     2521 gacacagcat aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg
     2581 cactgtcctc tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc
     2641 agcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2701 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2761 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2821 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2881 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2941 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3001 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3061 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3121 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3181 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3241 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3301 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3361 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3421 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3481 aaaaatgaag ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc
     3541 ctgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg
     3601 gggaaaagga agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag
     3661 agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt
     3721 tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg
     3781 tttcagttag cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga
     3841 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
     3901 cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc
     3961 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
     4021 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
     4081 agcaatatca cgggtagcca acgctatgtc ctgatagcga tccgccacac ccagccggcc
     4141 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     4201 gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag
     4261 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
     4321 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
     4381 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
     4441 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
     4501 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
     4561 ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt
     4621 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
     4681 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
     4741 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat
     4801 ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     4861 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     4921 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     4981 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     5041 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     5101 ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg
     5161 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5221 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5281 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5341 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5401 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5461 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5521 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5581 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5641 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5701 acaaaaattt aacgcgaatt tt
//
