LOCUS       sequence-42610-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Haataja L, Kaartinen V, Groffen J, Heisterkamp N
  TITLE     The small GTPase Rac3 interacts with the integrin-binding protein 
            CIB and promotes integrin alpha(IIb)beta(3)-mediated adhesion and 
            spreading.
  JOURNAL   J Biol Chem. 2002 Mar 8;277(10):8321-8. Epub 2001 Dec 27.
  PUBMED    11756406
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-03-8"; volume: "277(10)"; pages: "8321-8. Epub 
            2001 Dec 27"
COMMENT     Sequence Label: pCDNA3.1-CIB1-Myc
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(36..65)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(686..705)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(687..705)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(750..801)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(779..799)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcatggtgat ggtgatgatg accggtatgc atattcagat cctcttctga gatgagtttt
       61 tgttcgaagg gccctctaga caggacaatc ttaaaggagc tggcaaagtc tggagaacgg
      121 gagatgacgt gctggaactc agagaggttg atggttccat ccctgtcaat gtcagactcc
      181 tccaggatgt tgtcgatgag ctgcttcatc tcagacgcac taagccgtgt gtcctcgccc
      241 tctcccgtga ggcagttcac cagccggctc aggtcttctc tgttcaaggt tccgtcatca
      301 tcaaagtcaa agatgcggaa ggcataatgg gacttgatgt ctggcgtggc tgtgtcactg
      361 aacacactga ggagatccag gaagtcctca aagctaaggc tgtctttggc tggggatgtg
      421 gagaagaccc tgcagattcg ctccttgaag gggttggcct tgagctctgg aaggctgaga
      481 atctgctcga agggcacttg tgcccgaagt gacgactcca cgctccgctg ctcctgggga
      541 agcagctcac aaaaccgcct gtgggctagg aggatctcct gcttcgtcag gaacgtcaag
      601 tcctggtact cggccagcag ctccttggac aggcgactgc ccgagccccc catcggtacc
      661 aagcttaact agccagcttg ggtctcccta tagtgagtcg tattaatttc gataagccag
      721 taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc ccaccgtaca
      781 cgcctaccgc ccatttgcgt caatggggcg ganttgttac gacattttgg aaagtcccgt
      841 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggag
//
