LOCUS       sequence-426103-        8664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of the full length variants of the SHIP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Fafilek B, Balek L, Bosakova MK, Varecha M, Nita A, Gregor T, 
            Gudernova I, Krenova J, Ghosh S, Piskacek M, Jonatova L, Cernohorsky
            NH, Zieba JT, Kostas M, Haugsten EM, Wesche J, Erneux C, Trantirek 
            L, Krakow D, Krejci P
  TITLE     The inositol phosphatase SHIP2 enables sustained ERK activation 
            downstream of FGF receptors by recruiting Src kinases.
  JOURNAL   Sci Signal. 2018 Sep 18;11(548):eaap8608. doi: 
            10.1126/scisignal.aap8608.
  PUBMED    30228226
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2018-09-18"; volume: "11(548):eaap8608. doi"; 
            pages: " 10.1126/scisignal.aap8608"
COMMENT     Sequence Label: SHIP2-WT
FEATURES             Location/Qualifiers
     source          1..8664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2965..2976)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4824..4865
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4875..4892
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4921..4937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4921..4937)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4948..5570
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5147..5166)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(5719..6307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5799..5818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6636..6683
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6708..6727
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6915..7709)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6915..7709)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7026..7045)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7636..7655
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7744..8101)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7758..7893
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        7910..8101
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8058..8078
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(8103..8207)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8234..8662
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8321..8340)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8531..8552
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcctcggcc tgcggggcgc cgggcccggg gggcgccctg ggcagccagg
     1081 ccccctcctg gtaccaccgc gacctgagcc gggcggccgc ggaggagctg ctggcccggg
     1141 cgggccgcga tggcagcttc ctggtccgag acagcgagag cgtggcgggg gccttcgcgc
     1201 tctgcgtcct gtatcagaag catgtgcaca cgtatcgcat tctgcctgat ggagaagatt
     1261 tcttggctgt gcagacctcg cagggtgtgc ctgtgcgccg cttccagacc ctgggtgagc
     1321 tcatcggcct gtacgcccag cccaaccagg gccttgtgtg cgccctgctt cttcctgtag
     1381 agggtgagcg agagccggac ccaccggatg accgggatgc ctcagatggg gaggatgaga
     1441 agcccccgct gcccccgcgc tctggctcca ccagcatttc tgcccccact gggcccagca
     1501 gtcccctgcc agctcctgag actcccacag ctccagctgc tgagagtgct cccaatgggc
     1561 tgagcaccgt ctcgcacgac tacctgaaag gcagctatgg gctggacctg gaagctgtga
     1621 ggggtggagc cagccacctg ccccacctca cccgtaccct cgctacctca tgccggaggc
     1681 tgcacagtga ggtggacaag gtcctgtcag gcctggagat cctgtccaag gtgtttgacc
     1741 agcagagctc gcccatggtg acccgccttt tgcagcagca gaacctgcca cagacagggg
     1801 agcaggaact agagagcctg gtgctgaagc tgtcagtgct aaaggacttc ctgtcaggca
     1861 tccagaagaa ggccctgaag gccctacagg acatgagctc cacagcaccc ccagctccgc
     1921 agccatccac acgtaaggcc aagaccatcc ccgtgcaggc ctttgaggtg aagctagatg
     1981 tgaccctggg tgacctgacc aagattggga agtcacagaa gttcacgctg agcgtggatg
     2041 tggagggtgg gcggctggtg ctgctgcgga gacagcggga ctcccaggag gactggacca
     2101 ccttcacgca cgaccgcatc cgccagctca ttaagtccca gcgtgtccag aacaagctgg
     2161 gtgttgtgtt tgagaaggag aaggaccgga ctcagcgcaa ggacttcatc tttgtcagtg
     2221 cccggaagcg ggaggccttc tgccagctgt tgcagctcat gaagaacaag cactccaagc
     2281 aggacgagcc cgacatgatc tcagtcttca taggcacctg gaacatggga agtgtaccac
     2341 ctccaaaaaa cgtgacatcc tggttcacat cgaagggtct ggggaagacc ctggacgagg
     2401 tcacagtgac cataccccat gacatctatg tctttgggac ccaggagaac tcagtgggcg
     2461 accgcgagtg gctggaccta ctgcgcgggg gcctcaagga gcttacggat ctggattacc
     2521 gcccgattgc catgcaatca ctgtggaata tcaaggtggc agtgctggtc aagccagagc
     2581 acgagaaccg tatcagccat gtcagtacgt ccagtgtgaa gactggcatc gccaacaccc
     2641 tggggaacaa gggggctgtg ggcgtctcct tcatgtttaa tggcacctca tttggctttg
     2701 tgaattgtca cctcacctcg ggaaatgaga agacggctcg gaggaaccaa aactacttgg
     2761 acatcctgcg gctgctctcg ctgggcgacc ggcagctcaa tgcctttgac atctctctgc
     2821 gtttcacaca cctcttctgg tttggggacc tcaactaccg cctggacatg gatatccagg
     2881 agatcctgaa ctacatcagc aggaaagagt ttgagcccct cctcagggtg gaccagctca
     2941 acctggagcg ggagaagcac aaggtcttcc ttcgattcag tgaggaggag atctccttcc
     3001 cacccaccta ccgctatgag cggggttccc gggacacata tgcctggcac aagcagaagc
     3061 caactggggt ccggaccaat gtgccctcat ggtgtgaccg gattctgtgg aaatcctacc
     3121 ctgaaactca catcatctgc aattcttatg gttgcactga tgacatcgtc accagcgacc
     3181 attcccccgt gtttgggaca tttgaggttg gagttacctc ccagttcatc tccaagaaag
     3241 ggctctcaaa gacttcagac caggcctaca ttgagtttga gagcatcgag gccattgtga
     3301 agacagccag ccgcaccaag ttcttcatcg agttctactc tacctgcctg gaggaataca
     3361 agaagagctt tgagaatgat gcccagagca gtgacaacat caacttcctc aaagtgcagt
     3421 ggtcttcacg ccagctgccc acgctcaaac caattctggc tgatatcgag tacctgcagg
     3481 accagcacct cctgctcaca gtcaagtcca tggatggcta tgaatcctat ggggagtgtg
     3541 tggttgcact caaatccatg atcggcagca cggcccaaca gttcctgacc ttcctatccc
     3601 accgtggcga ggagacaggc aatatcagag gctccatgaa ggtgcgggtg cccacggagc
     3661 gcctgggcac ccgtgagcgg ctctacgagt ggatcagcat tgataaggat gaggcaggag
     3721 caaagagcaa agccccctct gtgtcccgag ggagccagga gcccaggtca gggagccgca
     3781 agccagcctt cacagaggcc tcctgcccgc tctccaggtt atttgaagaa ccagagaaac
     3841 cgccaccaac ggggaggccc ccagccccac cccgagcagc tccccgggag gagcccttga
     3901 cccccaggtt gaagccagag ggagctcctg aaccagaagg ggtggcggcc cccccaccca
     3961 agaacagctt caataaccct gcctactacg tccttgaagg ggtcccgcac cagctgctgc
     4021 ccccggagcc accctcgcct gccagggccc ctgtcccatc tgccaccaag aacaaagtgg
     4081 ccattacagt gcctgctcca cagcttgggc accaccggca ccctcgtgtg ggagagggga
     4141 gttcttcaga tgaggagtct ggaggcacac tgccccctcc agactttcca cctccaccac
     4201 tgccggactc agccatcttc ctgcccccca gcctggatcc tttaccaggg ccagtggtcc
     4261 ggggccgtgg tggggctgag gcccgtggcc caccacctcc caaggcccat ccaaggcctc
     4321 cactgccccc aggcccctca ccagccagca ctttcctggg ggaagtggcc agtggggatg
     4381 accggtcctg ctcggtgctg cagatggcca agacgctgag cgaggtggac tatgcccctg
     4441 ctgggcctgc acgctcagcg ctcctcccag gccccctgga gctgcagccc ccccggggac
     4501 tgccctcgga ctatggccgg cccctcagct tccctccacc ccgcatccgg gagagcatcc
     4561 aggaagacct ggcagaggag gctccgtgcc tgcagggcgg gcgggccagc gggctgggcg
     4621 aggcaggcat gagtgcctgg ctgcgggcca tcggcttgga gcgctatgag gagggcctgg
     4681 tgcataatgg ctgggacgac ctggagtttc tcagtgacat caccgaggag gacttggagg
     4741 aggctggggt gcaggacccg gctcacaagc gcctccttct ggacaccctg cagctcagca
     4801 agacgcgtac gcggccgctc gagggtaagc ctatccctaa ccctctcctc ggtctcgatt
     4861 ctacgcgtac cggtcatcat caccatcacc attgagctag cgtttaaacg gccggccgcg
     4921 gtcatagctg tttcctgaac agatcccggg tggcatccct gtgacccctc cccagtgcct
     4981 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     5041 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     5101 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     5161 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     5221 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     5281 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     5341 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     5401 accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc
     5461 cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt
     5521 ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg
     5581 ccagcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5641 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5701 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5761 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5821 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5881 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5941 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6001 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6061 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6121 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6181 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6241 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6301 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6361 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6421 ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg ctatggcagg
     6481 gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg gggggtgggg
     6541 tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg gggtatcgac
     6601 agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt
     6661 tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg
     6721 tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat ccccgcgctg
     6781 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     6841 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     6901 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     6961 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     7021 tcagcaatat cacgggtagc caacgctatg tcctgatagc gatccgccac acccagccgg
     7081 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     7141 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac
     7201 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     7261 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     7321 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     7381 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     7441 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     7501 agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc
     7561 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     7621 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     7681 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg
     7741 atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     7801 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     7861 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     7921 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     7981 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     8041 gcctggggac tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca
     8101 ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8161 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8221 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8281 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8341 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8401 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8461 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8521 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8581 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8641 taacaaaaat ttaacgcgaa tttt
//
