LOCUS       sequence-426109-        5521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose-inducible trpC (used in cross-feeding experiment).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Glass DS, Bren A, Vaisbourd E, Mayo A, Alon U
  TITLE     A synthetic differentiation circuit in Escherichia coli for 
            suppressing mutant takeover.
  JOURNAL   Cell. 2024 Jan 31:S0092-8674(24)00061-8. doi: 
            10.1016/j.cell.2024.01.024.
  PUBMED    38320549
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Jan 31:S0092-8674(24)00061-8. doi: 10.1016/j.cell.2024.01.024."
COMMENT     Sequence Label: pDSG550
FEATURES             Location/Qualifiers
     source          1..5521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..879)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        906..1190
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1113..1132
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    2859..2879
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2880..2937
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(join(3033..3824,3825..3893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3656..3675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3894..3965)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4419..4964)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4499..4518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5432..5450
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(5454..5497)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    5500..5521
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa cttttcggat caagctatga aggacgcaaa gagggaacta
     1261 aacactttac tagagtcaca caggactact agatgatgca aaccgtttta gcgaaaatcg
     1321 tcgcagacaa ggcgatttgg gtagaagccc gcaaacagca gcaaccgctg gccagttttc
     1381 agaatgaggt tcagccgagc acgcgacatt tttatgatgc gctacagggt gcgcgcacgg
     1441 cgtttattct ggagtgcaag aaagcgtcgc cgtcaaaagg cgtgatccgt gatgatttcg
     1501 atccagcacg cattgccgcc atttataaac attacgcttc ggcaatttcg gtgctgactg
     1561 atgagaaata ttttcagggg agctttaatt tcctccccat cgtcagccaa atcgccccgc
     1621 agccgatttt atgtaaagac ttcattatcg acccttacca gatctatctg gcgcgctatt
     1681 accaggccga tgcctgctta ttaatgcttt cagtactgga tgacgaccaa tatcgccagc
     1741 ttgccgccgt cgctcacagt ctggagatgg gggtgctgac cgaagtcagt aatgaagagg
     1801 aacaggagcg cgccattgca ttgggagcaa aggtcgttgg catcaacaac cgcgatctgc
     1861 gtgatttgtc gattgatctc aaccgtaccc gcgagcttgc gccgaaactg gggcacaacg
     1921 tgacggtaat cagcgaatcc ggcatcaata cttacgctca ggtgcgcgag ttaagccact
     1981 tcgctaacgg ttttctgatt ggttcggcgt tgatggccca tgacgatttg cacgccgccg
     2041 tgcgccgggt gttgctgggt gagaataaag tatgtggcct gacgcgtggg caagatgcta
     2101 aagcagctta tgacgcgggc gcgatttacg gtgggttgat ttttgttgcg acatcaccgc
     2161 gttgcgtcaa cgttgaacag gcgcaggaag tgatggctgc ggcaccgttg cagtatgttg
     2221 gcgtgttccg caatcacgat attgccgatg tggtggacaa agctaaggtg ttatcgctgg
     2281 cggcagtgca actgcatggt aatgaagaac agctgtatat cgatacgctg cgtgaagctc
     2341 tgccagcaca tgttgccatc tggaaagcat taagcgtcgg tgaaaccctg cccgcccgcg
     2401 agtttcagca cgttgataaa tatgttttag acaacggcca gggtggaagc gggcaacgtt
     2461 ttgactggtc actattaaat ggtcaatcgc ttggcaacgt tctgctggcg gggggcttag
     2521 gcgcagataa ctgcgtggaa gcggcacaaa ccggctgcgc cggacttgat tttaattctg
     2581 ctgtagagtc gcaaccgggc atcaaagacg cacgtctttt ggcctcggtt ttccagacgc
     2641 tgcgcgcata ttaataactc ggtaccaaag acgaacaata agacgctgaa aagcgtcttt
     2701 tttcgttttg gtcctttgaa gtttagggga tagtccaatt attgaaggcc tccctaacgg
     2761 ggggcctttt tttgtttctg gtctcccatc gagtggcgcg cttagttcct cgttttctct
     2821 cgttggaaga agaagaaacg agaaactaaa tactagagta ctagtagcgg ccgctgcagt
     2881 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
     2941 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3001 cgagcggtat cagctcactc aaaggcggta atttaccaat gcttaatcag tgaggcacct
     3061 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3121 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3181 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3241 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3301 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3361 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3421 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3481 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3541 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3601 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3661 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     3721 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     3781 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     3841 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     3901 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     3961 gaatgtattt agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag
     4021 ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact
     4081 ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga
     4141 ggcagacctc agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg
     4201 tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat
     4261 gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
     4321 gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat
     4381 acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct
     4441 gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa
     4501 agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg
     4561 tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag
     4621 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
     4681 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc
     4741 accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg
     4801 gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc
     4861 ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt
     4921 cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta
     4981 aggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5041 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5101 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5161 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5221 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5281 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5341 gtcctgcaac tttatccgcc tccatccagt ctattccatg gtgccacctg acgtctaaga
     5401 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
     5461 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga
     5521 g
//
