LOCUS       sequence-426112-       10373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for PiggyBac mediated integration and stable expression of 
            hFGFR3-G380R variant that is associated with Achondroplasia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10373)
  AUTHORS   Kimura T, Bosakova M, Nonaka Y, Hruba E, Yasuda K, Futakawa S, 
            Kubota T, Fafilek B, Gregor T, Abraham SP, Gomolkova R, Belaskova S,
            Pesl M, Csukasi F, Duran I, Fujiwara M, Kavkova M, Zikmund T, Kaiser
            J, Buchtova M, Krakow D, Nakamura Y, Ozono K, Krejci P
  TITLE     An RNA aptamer restores defective bone growth in FGFR3-related 
            skeletal dysplasia in mice.
  JOURNAL   Sci Transl Med. 2021 May 5;13(592):eaba4226. doi: 
            10.1126/scitranslmed.aba4226.
  PUBMED    33952673
REFERENCE   2  (bases 1 to 10373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2021-05-5"; volume: "13(592):eaba4226. doi"; pages: " 
            10.1126/scitranslmed.aba4226"
COMMENT     Sequence Label: TR01F/hFGFR3-G380R-V5
FEATURES             Location/Qualifiers
     source          1..10373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     1421..1440
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        1574..1777
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1728..1748
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1822..1841
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1822..1840
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4403..4444
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4454..4471
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4522..4643
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4891..5102
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5115..5383
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5412..5421
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5442..6104
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             6174..6227
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6228..6827
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6228..6247)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6724..6744
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6881..6901)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6943..7064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7827..7861
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8352..8368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8352..8368)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8365..8387)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8376..8392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8400..8406,8407..8424,8425..8430))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8445..8466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8583..8600)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8754..9342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8834..8853)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9513..10304,10305..10373))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10136..10155
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtattatgcc cagtacatga
     1381 ccttatggga ctttcctact tggcagtaca tctacgtaaa ctagcaaaat aggctgtccc
     1441 cagtgcaagt gcaggtgcca gaacatttct ctggcctaac tggccggtac ctgagctcgc
     1501 tagcgggaat ttccggggac tttccgggaa tttccgggga ctttccggga atttccagat
     1561 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     1621 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     1681 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     1741 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     1801 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagtt
     1861 aagcttggta ccgagctcgg atccagtacc cttcacccac gaccccacca tgggcgcccc
     1921 tgcctgcgcc ctcgcgctct gcgtggccgt ggccatcgtg gccggcgcct cctcggagtc
     1981 cttggggacg gagcagcgcg tcgtggggcg agcggcagaa gtcccgggcc cagagcccgg
     2041 ccagcaggag cagttggtct tcggcagcgg ggatgctgtg gagctgagct gtcccccgcc
     2101 cgggggtggt cccatggggc ccactgtctg ggtcaaggat ggcacagggc tggtgccctc
     2161 ggagcgtgtc ctggtggggc cccagcggct gcaggtgctg aatgcctccc acgaggactc
     2221 cggggcctac agctgccggc agcggctcac gcagcgcgta ctgtgccact tcagtgtgcg
     2281 ggtgacagac gctccatcct cgggagatga cgaagacggg gaggacgagg ctgaggacac
     2341 aggtgtggac acaggggccc cttactggac acggcccgag cggatggaca agaagctgct
     2401 ggccgtgccg gccgccaaca ccgtccgctt ccgctgccca gccgctggca accccactcc
     2461 ctccatctcc tggctgaaga acggcaggga gttccgcggc gagcaccgca ttggaggcat
     2521 caagctgcgg catcagcagt ggagcctggt catggaaagc gtggtgccct cggaccgcgg
     2581 caactacacc tgcgtcgtgg agaacaagtt tggcagcatc cggcagacgt acacgctgga
     2641 cgtgctggag cgctccccgc accggcccat cctgcaggcg gggctgccgg ccaaccagac
     2701 ggcggtgctg ggcagcgacg tggagttcca ctgcaaggtg tacagtgacg cacagcccca
     2761 catccagtgg ctcaagcacg tggaggtgaa cggcagcaag gtgggcccgg acggcacacc
     2821 ctacgttacc gtgctcaaga cggcgggcgc taacaccacc gacaaggagc tagaggttct
     2881 ctccttgcac aacgtcacct ttgaggacgc cggggagtac acctgcctgg cgggcaattc
     2941 tattgggttt tctcatcact ctgcgtggct ggtggtgctg ccagccgagg aggagctggt
     3001 ggaggctgac gaggcgggca gtgtgtatgc aggcatcctc agctacaggg tgggcttctt
     3061 cctgttcatc ctggtggtgg cggctgtgac gctctgccgc ctgcgcagcc cccccaagaa
     3121 aggcctgggc tcccccaccg tgcacaagat ctcccgcttc ccgctcaagc gacaggtgtc
     3181 cctggagtcc aacgcgtcca tgagctccaa cacaccactg gtgcgcatcg caaggctgtc
     3241 ctcaggggag ggccccacgc tggccaatgt ctccgagctc gagctgcctg ccgaccccaa
     3301 atgggagctg tctcgggccc ggctgaccct gggcaagccc cttggggagg gctgcttcgg
     3361 ccaggtggtc atggcggagg ccatcggcat tgacaaggac cgggccgcca agcctgtcac
     3421 cgtagccgtg aagatgctga aagacgatgc cactgacaag gacctgtcgg acctggtgtc
     3481 tgagatggag atgatgaaga tgatcgggaa acacaaaaac atcatcaacc tgctgggcgc
     3541 ctgcacgcag ggcgggcccc tgtacgtgct ggttgagtac gcggccaagg gtaacctgcg
     3601 ggagtttctg cgggcgcggc ggcccccggg cctggactac tccttcgaca cctgcaagcc
     3661 gcccgaggag cagctcacct tcaaggacct ggtgtcctgt gcctaccagg tggcccgggg
     3721 catggagtac ttggcctccc agaagtgcat ccacagggac ctggctgccc gcaatgtgct
     3781 ggtgaccgag gacaacgtga tgaagatcgc agacttcggg ctggcccggg acgtgcacaa
     3841 cctcgactac tacaagaaga caaccaacgg ccggctgccc gtgaagtgga tggcgcctga
     3901 ggccttgttt gaccgagtct acactcacca gagtgacgtc tggtcctttg gggtcctgct
     3961 ctgggagatc ttcacgctgg ggggctcccc gtaccccggc atccctgtgg aggagctctt
     4021 caagctgctg aaggagggcc accgcatgga caagcccgcc aactgcacac acgacctgta
     4081 catgatcatg cgggagtgct ggcatgccgc gccctcccag aggcccacct tcaagcagct
     4141 ggtggaggac ctggaccgtg tccttaccgt gacgtccacc gacgagtacc tggacctgtc
     4201 ggcgcctttc gagcagtact ccccgggtgg ccaggacacc cccagctcca gctcctcagg
     4261 ggacgactcc gtgtttgccc acgacctgct gcccccggcc ccacccagca gtgggggctc
     4321 gcggacggcc aagggtcaag acaattctgc agatatccag cacagtggcg gccgctcgag
     4381 tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
     4441 tacgcgtacc ggtcatcatc accatcacca ttgagtttaa accctagagt cggggcggcc
     4501 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     4561 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     4621 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     4681 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     4741 ataaggatcc gtttgcgtat tgggcgctct tccgctgatc tgcgcagcac catggcctga
     4801 aataacctct gaaagaggaa cttggttagc taccttctaa atcggatccg cggccgcgaa
     4861 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     4921 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa
     4981 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     5041 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     5101 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc
     5161 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg
     5221 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc
     5281 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac
     5341 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc
     5401 ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt
     5461 gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca
     5521 cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca
     5581 gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca
     5641 gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca
     5701 tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg
     5761 gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct
     5821 tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata
     5881 acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct
     5941 tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg
     6001 ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca
     6061 tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt
     6121 cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca
     6181 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca
     6241 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     6301 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     6361 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6421 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6481 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6541 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6601 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     6661 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     6721 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     6781 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg
     6841 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6901 gtggatacgc tgctttaatg cctttgtatc atgcgttaac taaacttgtt tattgcagct
     6961 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7021 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggaattga
     7081 ctcaaatgat gtcaattagt ctatcagaag ctcatctggt ctcccttccg ggggacaaga
     7141 catccctgtt taatatttaa acagcagtgt tcccaaactg ggttcttata tcccttgctc
     7201 tggtcaacca ggttgcaggg tttcctgtcc tcacaggaac gaagtcccta aagaaacagt
     7261 ggcagccagg tttagccccg gaattgactg gattcctttt ttagggccca ttggtatggc
     7321 tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag
     7381 gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg
     7441 ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc tgccccctag
     7501 cgggggaggg acgtaattac atccctgggg gctttggggg ggggctgtcc ctgatatcta
     7561 taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata
     7621 attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact
     7681 cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg
     7741 ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag
     7801 aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg
     7861 gttaatctag ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc
     7921 aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga
     7981 agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg
     8041 cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt
     8101 cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt
     8161 cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg
     8221 tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc
     8281 tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcttg
     8341 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8401 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8461 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8521 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8581 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8641 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8701 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8761 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8821 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8881 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8941 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9001 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9061 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9121 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9181 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9241 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9301 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9361 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9421 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9481 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9541 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9601 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9661 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9721 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9781 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9841 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9901 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9961 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10021 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10081 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10141 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10201 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10261 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10321 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact cat
//
