LOCUS       sequence-426113-        5450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  precut biphasic target plasmid version 1.0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Glass DS, Bren A, Vaisbourd E, Mayo A, Alon U
  TITLE     A synthetic differentiation circuit in Escherichia coli for 
            suppressing mutant takeover.
  JOURNAL   Cell. 2024 Jan 31:S0092-8674(24)00061-8. doi: 
            10.1016/j.cell.2024.01.024.
  PUBMED    38320549
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Jan 31:S0092-8674(24)00061-8. doi: 10.1016/j.cell.2024.01.024."
COMMENT     Sequence Label: pDSG553
FEATURES             Location/Qualifiers
     source          1..5450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..879)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        906..1190
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1113..1132
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    2788..2808
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2809..2866
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(join(2962..3753,3754..3822))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3585..3604
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3823..3894)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4348..4893)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4428..4447)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5361..5379
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(5383..5426)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    5429..5450
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa cttttcggat caagctatga aggaagaaga agaaacgaga
     1261 aactaaatac tagagtcaca caggactact agatgatgca aaccgtttta gcgaaaatcg
     1321 tcgcagacaa ggcgatttgg gtagaagccc gcaaacagca gcaaccgctg gccagttttc
     1381 agaatgaggt tcagccgagc acgcgacatt tttatgatgc gctacagggt gcgcgcacgg
     1441 cgtttattct ggagtgcaag aaagcgtcgc cgtcaaaagg cgtgatccgt gatgatttcg
     1501 atccagcacg cattgccgcc atttataaac attacgcttc ggcaatttcg gtgctgactg
     1561 atgagaaata ttttcagggg agctttaatt tcctccccat cgtcagccaa atcgccccgc
     1621 agccgatttt atgtaaagac ttcattatcg acccttacca gatctatctg gcgcgctatt
     1681 accaggccga tgcctgctta ttaatgcttt cagtactgga tgacgaccaa tatcgccagc
     1741 ttgccgccgt cgctcacagt ctggagatgg gggtgctgac cgaagtcagt aatgaagagg
     1801 aacaggagcg cgccattgca ttgggagcaa aggtcgttgg catcaacaac cgcgatctgc
     1861 gtgatttgtc gattgatctc aaccgtaccc gcgagcttgc gccgaaactg gggcacaacg
     1921 tgacggtaat cagcgaatcc ggcatcaata cttacgctca ggtgcgcgag ttaagccact
     1981 tcgctaacgg ttttctgatt ggttcggcgt tgatggccca tgacgatttg cacgccgccg
     2041 tgcgccgggt gttgctgggt gagaataaag tatgtggcct gacgcgtggg caagatgcta
     2101 aagcagctta tgacgcgggc gcgatttacg gtgggttgat ttttgttgcg acatcaccgc
     2161 gttgcgtcaa cgttgaacag gcgcaggaag tgatggctgc ggcaccgttg cagtatgttg
     2221 gcgtgttccg caatcacgat attgccgatg tggtggacaa agctaaggtg ttatcgctgg
     2281 cggcagtgca actgcatggt aatgaagaac agctgtatat cgatacgctg cgtgaagctc
     2341 tgccagcaca tgttgccatc tggaaagcat taagcgtcgg tgaaaccctg cccgcccgcg
     2401 agtttcagca cgttgataaa tatgttttag acaacggcca gggtggaagc gggcaacgtt
     2461 ttgactggtc actattaaat ggtcaatcgc ttggcaacgt tctgctggcg gggggcttag
     2521 gcgcagataa ctgcgtggaa gcggcacaaa ccggctgcgc cggacttgat tttaattctg
     2581 ctgtagagtc gcaaccgggc atcaaagacg cacgtctttt ggcctcggtt ttccagacgc
     2641 tgcgcgcata ttaataactc ggtaccaaag acgaacaata agacgctgaa aagcgtcttt
     2701 tttcgttttg gtcctttgaa gtttagggga tagtccaatt attgaaggcc tccctaacgg
     2761 ggggcctttt tttgtttctg gtctccctac tagtagcggc cgctgcagtc cggcaaaaaa
     2821 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat
     2881 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2941 agctcactca aaggcggtaa tttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3001 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3061 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3121 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3181 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3241 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3301 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3361 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3421 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3481 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3541 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3601 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3661 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3721 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3781 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3841 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3901 gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac
     3961 caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt tctggctgga
     4021 tgatggggcg attcaggcct ggtatgagtc agcaacacct tcttcacgag gcagacctca
     4081 gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg
     4141 cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga
     4201 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
     4261 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
     4321 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
     4381 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
     4441 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     4501 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     4561 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     4621 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     4681 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     4741 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     4801 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     4861 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa ggggtctgac
     4921 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4981 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5041 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5101 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5161 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5221 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5281 ttatccgcct ccatccagtc tattccatgg tgccacctga cgtctaagaa accattatta
     5341 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     5401 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag
//
