LOCUS       sequence-426114-        4116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DAPG-inducible folA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Glass DS, Bren A, Vaisbourd E, Mayo A, Alon U
  TITLE     A synthetic differentiation circuit in Escherichia coli for 
            suppressing mutant takeover.
  JOURNAL   Cell. 2024 Jan 31:S0092-8674(24)00061-8. doi: 
            10.1016/j.cell.2024.01.024.
  PUBMED    38320549
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Jan 31:S0092-8674(24)00061-8. doi: 10.1016/j.cell.2024.01.024."
COMMENT     Sequence Label: pDSG488
FEATURES             Location/Qualifiers
     source          1..4116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(21..92)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             1110..1121
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1128..1364
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     misc_feature    1368..1388
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1389..1446
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(1604..2419)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1604..2419)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2327..2346
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2411..2430
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(3014..3559)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3094..3113)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4027..4045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(4049..4092)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    4095..4116
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 cttaagctgc gagtgggccc gagagcgttc accgacaaac aacagataaa acgaaaggcc
       61 cagtctttcg actgagcctt tcgttttatt tgatgcctgg ggagtgtgag tgggaggtct
      121 gtatgtacgt cgagtacctt tggtcgaaaa aaaaagcccg cactgtcagg tgcgggcttt
      181 tttctgtgtt tccttaacgc tgtgtacccg gacaaacacc attaatcagc aggaaggtaa
      241 attcttcaat atcctgttca acggtcagct gttcggtcag taggcgatac caacaaaaac
      301 caaaaatcat atccagcagc agttcacgat tggtatcttt cggcagttca ccattgctaa
      361 tggcattttc aaccagtttt ttcggcatct cacgacgacg ttccataaac tgatctttca
      421 gctgggtcag ggttgcaggg tccagctgtg cttctgcaat aacacaacga aatgcttcac
      481 cacaaatggt ttcacgccaa actttccaca gattacgcag cagaaaatcc agatcggctt
      541 taaagctacc cagatccgga aatttacgca cctgttcgct ttcattttca tacacttcgg
      601 caatcagtgc tgctttattg gtccaccaac gataaatggt cggtttgctt gcaccggcac
      661 gacgtgcaac gctttcaatg ctcagaccgc tataaccaca ttctttcagg atttcaatgg
      721 tgctggtcag aattgcttta tgggtatgcg gactacgcag gctaccaatg ctgctacggc
      781 tcggggtacg tgccatctag tatttctccc ttcgccgccg attgattcag aaagttaaac
      841 aaaattattt gtagagggaa accgttgtgg tctccctgaa tatattatac gagccttatg
      901 catgcccgta aagttatcca gcaaccactc atagacctag ggcagcagat agggacgacg
      961 tggtgttagc tgtgccgtcg gaattgctat agcccttaac gcatgagaaa gcccccggaa
     1021 gatcaccttc cgggggcttt tttattgcgc tctgattcgt taccaattga catgatacga
     1081 aacgtaccgt atcgttaagg ttactagaga aagaggagaa atactagatg ggtcaaagta
     1141 gcgatgaagc caacgctccc gttgcagggc agtttgcgct tcccctgagt gccacctttg
     1201 gcttagggga tcgcgtacgc aagaaatctg gtgccgcttg gcagggtcaa gtcgtcggtt
     1261 ggtattgcac aaaactcact cctgaaggct atgcggtcga gtccgaatcc cacccaggct
     1321 cagtgcaaat ttatcctgtg gctgcacttg aacgtgtggc ctaataatac tagtagcggc
     1381 cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
     1441 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1501 cggctgcggc gagcggtatc agctcactca aaggcggtaa tctcgagtcc cgtcaagtca
     1561 gcgtaatgct ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga
     1621 gcatcaaatg aaactgcaat ttattcatat caggattatc gataccatat ttttgaaaaa
     1681 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     1741 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     1801 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     1861 gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     1921 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     1981 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     2041 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     2101 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     2161 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     2221 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     2281 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     2341 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctggag caagacgttt
     2401 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     2461 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
     2521 cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc
     2581 ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct
     2641 acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc
     2701 aggcctggta tgagtcagca acaccttctt cacgaggcag acctcagcgc tagcggagtg
     2761 tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg
     2821 agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc
     2881 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa
     2941 cggggcggag atttcctgga agatgccagg aagatactta acagggaagt gagagggccg
     3001 cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct
     3061 caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg
     3121 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     3181 ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     3241 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat
     3301 cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat
     3361 tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag
     3421 ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag
     3481 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg
     3541 cagaccaaaa cgatctcaag aagatcatct tattaagggg tctgacgctc agtggaacga
     3601 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3661 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3721 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3781 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3841 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3901 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3961 ccagtctatt ccatggtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     4021 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     4081 aaggatgatt tctggaattc gcggccgctt ctagag
//
