LOCUS       sequence-426131-        4985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human DDA1. Untagged..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-LIC_DDA1:1-102
FEATURES             Location/Qualifiers
     source          1..4985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    91..225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(115..134)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     169..188
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(724..815)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(782..799)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(1167..1172,1173..1189,1190..1195)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1365..1898
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  1965..2189
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(2323..2340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2494..3082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2574..2593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3253..4044,4045..4113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3876..3895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4114..4218)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4244..4698)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4381..4402)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4592..4611
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  join(4882..4985,1..62)
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc acttatctgg
       61 ttcggatctc ctaggcgata tcagtgatca gatccagaca tgataagata cattgatgag
      121 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
      181 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      241 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      301 ctctacaaat gtggtatggc tgattatgat cctctagtac ttctcgacaa gcttcgtcat
      361 caagtgtcct cgtgcatgtc tgggctgtcg gtccgcgcca ccttgcgggg aggtgcggag
      421 ctctcgcctt ccagctccac ttgctcctgg tctctcttct tggcagcgtt ctttttgtcc
      481 cattgctgat gcaggtagcg caggaggatg tttgtctttt ctgtcacgat gatctgttca
      541 gacgggtact cgcgggtagg caggtagact gagggccgtc ggttcgaggc tttgcacacg
      601 gagtccgcgt gaaatcgact aaaattgctt ttgttgtaga caggcagtcc tttcaaaaaa
      661 tctgccatgg ttttcggacc gagatccgcg cccgatggtg ggacggtatg aataatccgg
      721 aatatttata ggttttttta ttacaaaact gttacgaaaa cagtaaaata cttatttatt
      781 tgcgagatgg ttatcatttt aattatctcc atgatctatt aatattccgg agtatacgta
      841 gccaaccact agaactatag ctagagtcct gggcgaacaa acgatgctcg ccttccagaa
      901 aaccgaggat gcgaaccact tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc
      961 gaggtcttcc gatctcctga agccagggca gatccgtgca cagcaccttg ccgtagaaga
     1021 acagcaaggc cgccaatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca
     1081 gccaggacag aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt
     1141 ggaaacggat gaaggcacga acccagttga cataagcctg ttcggttcgt aaactgtaat
     1201 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1261 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttgtacag tctatgcctc
     1321 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     1381 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aggtggctca agtatgggca
     1441 tcattcgcac atgtaggctc ggccctgacc aagtcaaatc catgcgggct gctcttgatc
     1501 ttttcggtcg tgagttcgga gacgtagcca cctactccca acatcagccg gactccgatt
     1561 acctcgggaa cttgctccgt agtaagacat tcatcgcgct tgctgccttc gaccaagaag
     1621 cggttgttgg cgctctcgcg gcttacgttc tgcccaagtt tgagcagccg cgtagtgaga
     1681 tctatatcta tgatctcgca gtctccggcg agcaccggag gcagggcatt gccaccgcgc
     1741 tcatcaatct gctcaagcat gaggccaacg cgcttggtgc ttatgtgatc tacgtgcaag
     1801 cagattacgg tgacgatccc gcagtggctc tctatacaaa gttgggcata cgggaagaag
     1861 tgatgcactt tgatatcgac ccaagtaccg ccacctaaca attcgttcaa gccgagatcg
     1921 gcttcccggc cgcggagttg ttcggtaaat tgtcacaacg ccgcgaatat agtctttacc
     1981 atgcccttgg ccacgcccct ctttaatacg acgggcaatt tgcacttcag aaaatgaaga
     2041 gtttgcttta gccataacaa aagtccagta tgctttttca cagcataact ggactgattt
     2101 cagtttacaa ctattctgtc tagtttaaga ctttattgtc atagtttaga tctattttgt
     2161 tcagtttaag actttattgt ccgcccacac ccgcttacgc agggcatcca tttattactc
     2221 aaccgtaacc gattttgcca ggttacgcgg ctggtctatg cggtgtgaaa taccgcacag
     2281 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     2341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3181 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3241 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3301 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3361 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3421 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3481 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3541 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3601 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3661 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3721 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3781 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3841 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3901 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3961 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4021 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4081 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     4261 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     4321 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     4381 ccagtttgga acaagagtcc actattaaga acgtggactc caacgtcaaa gggcgaaaaa
     4441 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     4501 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     4561 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     4621 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     4681 cgccgctaca gggcgcgtcc cattcgccat tcaggctgca aataagcgtt gatattcagt
     4741 caattacaaa cattaataac gaagagatga cagaaaaatt ttcattctgt gacagagaaa
     4801 aagtagccga agatgacggt ttgtcacatg gagttggcag gatgtttgat taaaaacata
     4861 acaggaagaa aaatgccccg ctgtgggcgg acaaaatagt tgggaactgg gaggggtgga
     4921 aatggagttt ttaaggatta tttagggaag agtgacaaaa tagatgggaa ctgggtgtag
     4981 cgtcg
//
