LOCUS       sequence-426133-        5717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human WDR12 fragment T90 to T417. N-terminal 6X
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_WDR12:90-417
FEATURES             Location/Qualifiers
     source          1..5717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    726..860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(763..782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     817..836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  889..1054
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1238..1692
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1325..1344)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1534..1555
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1718..1822
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1823..1891,1892..2683)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2041..2060)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2854..3442
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3343..3362
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3596..3613
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3747..3971
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4038..4571)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4741..4746,4747..4763,4764..4769))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5121..5212
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5137..5154
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5271..5288
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5301..5321
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gatgaagaag atgaaatgga ggagtccaca aatcgaccaa gaaagaaaca gaagacagaa
       61 cagttgggac taacaaggac tcccatagtg accctctctg gccacatgga ggcagtttcc
      121 tcagttctgt ggtcagatgc tgaagaaatc tgcagtgcat cttgggacca tacaattaga
      181 gtgtgggatg ttgagtctgg cagtcttaag tcaactttga caggaaataa agtgtttaat
      241 tgtatctcct attctccact ttgtaaacgt ttagcatctg gaagcacaga ttcccatatc
      301 agactgtggg atccccgaac taaagatggt tctttggtgt cgctgtccct aacgtcacat
      361 actggttggg tgacatcagt aaaatggtct cctacccatg aacagcagct gatttcagga
      421 tctttagata acattgttaa gctgtgggat acaagaagtt gtaaggctcc tctctatgat
      481 ctggctgctc atgaagacaa agttctgagt gtagactgga cagacacagg gctacttctg
      541 agtggaggag cagacaataa attgtattcc tacagatatt cacctacctg atgacgaagc
      601 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
      661 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
      721 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      781 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      841 tatcttatca tgtctggatc tgatcactga tatcgcctag gagatccgaa ccagataagt
      901 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
      961 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
     1021 ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt
     1081 ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct
     1141 ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga
     1201 atatcaacgc ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     1261 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1321 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1381 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1441 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1501 cgccctttga cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa
     1561 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     1621 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     1681 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1741 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1801 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1861 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1921 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1981 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2041 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     2101 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     2161 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     2221 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2281 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2341 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2401 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2461 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2521 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2581 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2641 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2701 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2761 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2821 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2881 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2941 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3001 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3061 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3121 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3181 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3241 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3301 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3361 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3421 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3481 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3541 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3601 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     3661 cggtatttca caccgcatag accagccgcg taacctggca aaatcggtta cggttgagta
     3721 ataaatggat gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa
     3781 atagatctaa actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc
     3841 agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc
     3901 attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatggtaa
     3961 agactatatt cgcggcgttg tgacaattta ccgaacaact ccgcggccgg gaagccgatc
     4021 tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg catcacttct
     4081 tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta atctgcttgc
     4141 acgtagatca cataagcacc aagcgcgttg gcctcatgct tgagcagatt gatgagcgcg
     4201 gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatagat atagatctca
     4261 ctacgcggct gctcaaactt gggcagaacg taagccgcga gagcgccaac aaccgcttct
     4321 tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga gcaagttccc gaggtaatcg
     4381 gagtccggct gatgttggga gtaggtggct acgtctccga actcacgacc gaaaagatca
     4441 agagcagccc gcatggattt gacttggtca gggccgagcc tacatgtgcg aatgatgccc
     4501 atacttgagc cacctaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     4561 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     4621 atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     4681 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     4741 agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt
     4801 gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg
     4861 aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc
     4921 tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg
     4981 tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg
     5041 gaaggcgagc atcgtttgtt cgcccaggac tctagctata gttctagtgg ttggctacgt
     5101 atactccgga atattaatag atcatggaga taattaaaat gataaccatc tcgcaaataa
     5161 ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa atattccgga
     5221 ttattcatac cgtcccacca tcgggcgcgg atctcggtcc gaaaaccatg catcatcatc
     5281 atcatcacag cagcggcaga gaaaacttgt atttccaggg cactgcaccc cagccagagc
     5341 aatgcatggc acatgatgac tggatcagtt caattaaagg ggcagaggaa tggatcttga
     5401 ctggttctta tgataagact tctcggatct ggtccttgga aggagcatca ataatgacaa
     5461 ttgtgggaca tacggatgtt gtaaaagatg tggcctgggt gaaaaaagat agtttgtcct
     5521 gcttattatt gagtgcttct atggatcaga ctattctctt atgggagtgg aatgtagaga
     5581 gaaacaaagt gaaagcccta cactgctgta gaggtcatgc tggaagtgta gattctatag
     5641 ctgttgatgg ctcaggaact aaattttgca gtggctcctg ggataagatg ctaaagatct
     5701 ggtctacagt ccctaca
//
