LOCUS       sequence-426135-        5792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human AAMP fragment D82 to R434. N-terminal 6X 
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_AAMP:82-434
FEATURES             Location/Qualifiers
     source          1..5792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(286..819)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(989..994,995..1011,1012..1017))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1369..1460
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1385..1402
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1519..1536
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1549..1569
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2766..2900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2803..2822)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2857..2876
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2929..3094
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3278..3732
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3365..3384)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3574..3595
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3758..3862
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3863..3931,3932..4723)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4081..4100)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4894..5482
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5383..5402
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5636..5653
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  join(5787..5792,1..219)
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 cggacaataa agtcttaaac tgaacaaaat agatctaaac tatgacaata aagtcttaaa
       61 ctagacagaa tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga
      121 cttttgttat ggctaaagca aactcttcat tttctgaagt gcaaattgcc cgtcgtatta
      181 aagaggggcg tggccaaggg catggtaaag actatattcg cggcgttgtg acaatttacc
      241 gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact
      301 tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac
      361 tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc
      421 ctcatgcttg agcagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga
      481 gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta
      541 agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt
      601 actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac
      661 gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg
      721 gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg
      781 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
      841 acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc
      901 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
      961 gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt atgtcaactg
     1021 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1081 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg
     1141 tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg
     1201 gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga
     1261 tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg cccaggactc
     1321 tagctatagt tctagtggtt ggctacgtat actccggaat attaatagat catggagata
     1381 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
     1441 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggat
     1501 ctcggtccga aaaccatgca tcatcatcat catcacagca gcggcagaga aaacttgtat
     1561 ttccagggcg atagcgaggt cacctttgca ttgcactcag catctgtgtt ttgtgtgagc
     1621 ctggacccca agaccaatac cttggcagtg accgggggtg aagatgacaa agccttcgta
     1681 tggcggctca gcgatgggga gctgctcttt gagtgtgcag gccataaaga ctctgtgact
     1741 tgtgctggtt tcagccatga ctccactcta gtggccacag gggacatgag tggcctcttg
     1801 aaagtgtggc aggtggacac taaggaggag gtctggtcct ttgaagcggg agacctggag
     1861 tggatggagt ggcatcctcg ggcacctgtc ctgttggcgg gcacagctga cggcaacacc
     1921 tggatgtgga aagtcccgaa tggtgactgc aagaccttcc agggtcccaa ctgcccagcc
     1981 acctgtggcc gagtcctccc tgatgggaag agagctgtgg taggctatga agatgggacc
     2041 atcaggattt gggacctgaa gcagggaagc cctatccatg tactgaaagg gactgagggt
     2101 caccagggcc cactcacctg tgttgctgcc aaccaggatg gcagcttgat cctaactggc
     2161 tctgtggact gccaggccaa gctggtcagt gccaccaccg gcaaggtggt gggtgttttt
     2221 agacctgaga ctgtggcctc ccagcccagc ctgggagaag gggaggagag tgagtccaac
     2281 tcggtggagt ccttgggctt ctgcagtgtg atgcccctgg cagctgttgg ctacctggat
     2341 gggaccttgg ccatctatga cctggctacg cagactctta ggcatcagtg tcagcaccag
     2401 tcgggcatcg tgcagctgct gtgggaggca ggcactgccg tggtatatac ctgcagcctg
     2461 gatggcatcg tgcgcctctg ggacgcccgg accggccgcc tgcttactga ctaccggggc
     2521 cacacggctg agatcctgga ctttgccctc agcaaagatg cctccctggt ggtgaccacg
     2581 tcaggagacc acaaagcgaa agtattttgt gtccaaaggc ctgaccgttg atgacgaagc
     2641 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
     2701 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     2761 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     2821 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     2881 tatcttatca tgtctggatc tgatcactga tatcgcctag gagatccgaa ccagataagt
     2941 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
     3001 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
     3061 ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt
     3121 ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct
     3181 ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga
     3241 atatcaacgc ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     3301 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3361 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3421 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3481 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3541 cgccctttga cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa
     3601 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     3661 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3721 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3781 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3841 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3901 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3961 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4021 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4081 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4141 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4201 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4261 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4321 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4381 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4441 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4501 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4561 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4621 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4681 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4741 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4801 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4861 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4921 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4981 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5041 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5101 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5161 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5221 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5281 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5341 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5401 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5461 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5521 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5581 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5641 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     5701 cggtatttca caccgcatag accagccgcg taacctggca aaatcggtta cggttgagta
     5761 ataaatggat gccctgcgta agcgggtgtg gg
//
