LOCUS       sequence-426139-        6200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human CORO1B. N-terminal 6X His tag
            with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_CORO1B:1-489
FEATURES             Location/Qualifiers
     source          1..6200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1270..1404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1307..1326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1361..1380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1433..1598
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1782..2236
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1869..1888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2078..2099
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2262..2366
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2367..2435,2436..3227)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2585..2604)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3398..3986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3887..3906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4140..4157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4291..4515
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4582..5115)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5285..5290,5291..5307,5308..5313))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5665..5756
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5681..5698
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5815..5832
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5845..5865
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cccagagaac gggctgacct ccccgctgac agagccggtg gtggtactgg aggggcacac
       61 caagcgagtg ggcatcatcg cctggcaccc cacggcccga aacgtgctgc tcagtgcagg
      121 ctgcgacaac gtggtactca tctggaatgt gggcacagcg gaggagctgt accgcctgga
      181 cagcctgcac cctgacctca tctacaatgt cagctggaac cacaatggca gcctgttttg
      241 ctcagcatgc aaggacaaga gcgtgcgcat catcgacccc cgtcggggca ccctggtggc
      301 agagcgggag aaggctcatg agggggcccg gcccatgcgg gccatcttcc tggcagatgg
      361 caaggtgttc accacaggct tcagccgaat gagcgagcgg cagctggcgc tctgggaccc
      421 agaaaacctc gaggaaccca tggccctgca ggaactggac tcgagcaacg gggccctgct
      481 gcccttctac gaccccgaca ccagtgtggt ctacgtctgc ggcaagggtg actccagcat
      541 ccggtacttt gagatcacag aggagcctcc ctacatccac ttcctgaaca cgttcaccag
      601 caaggagccg cagcggggta tgggcagcat gcccaagcgg ggcctggagg tcagcaagtg
      661 cgagatcgcc cggttctaca aactgcatga gcgcaagtgt gagcccatcg tcatgactgt
      721 gccaagaaag tcggacctct tccaggatga tctgtacccc gacacagccg ggcccgaggc
      781 agccctggag gctgaggagt gggtgagcgg gcgggatgcc gacccgatcc tcatctcact
      841 gcgggaggcc tacgtgccca gcaagcagcg ggacctgaag atcagccggc gcaacgtgtt
      901 gtctgacagc cggcccgcca tggccccggg ctcctcccac ctaggggccc ccgcctccac
      961 caccactgct gctgatgcca cccccagcgg cagcctggcc agagccgggg aggctgggaa
     1021 gctggaggag gtgatgcagg agctgcgggc cctgagggcg ctggtcaagg agcagggcga
     1081 ccgcatctgc cgcctggagg agcagctggg ccgcatggag aacggggatg cgtgatgacg
     1141 aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta
     1201 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     1261 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1321 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1381 aatgtatctt atcatgtctg gatctgatca ctgatatcgc ctaggagatc cgaaccagat
     1441 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc
     1501 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt
     1561 ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt
     1621 atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt
     1681 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
     1741 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     1801 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     1861 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     1921 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     1981 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     2041 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
     2101 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2161 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2221 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2281 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2341 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2401 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2461 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2521 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2581 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2641 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2701 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2761 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2821 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2881 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2941 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3001 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3061 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3121 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3181 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3241 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3301 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3361 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3421 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3481 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3541 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3601 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3661 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3721 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3781 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3841 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3901 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3961 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4021 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4081 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4141 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     4201 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     4261 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     4321 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     4381 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     4441 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     4501 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     4561 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     4621 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     4681 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagca gattgatgag
     4741 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     4801 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     4861 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     4921 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     4981 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     5041 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     5101 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     5161 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     5221 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     5281 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     5341 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct
     5401 ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt
     5461 cttctacggc aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg
     5521 gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt
     5581 tctggaaggc gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct
     5641 acgtatactc cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa
     5701 ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc
     5761 cggattattc ataccgtccc accatcgggc gcggatctcg gtccgaaaac catgcatcat
     5821 catcatcatc acagcagcgg cagagaaaac ttgtatttcc agggcatgtc cttccgcaaa
     5881 gtggtccggc agagcaaatt ccggcatgtg ttcgggcagc cggtcaagaa cgaccagtgc
     5941 tatgaggaca ttcgcgtgtc ccgtgttacc tgggacagca ccttctgcgc cgtcaacccc
     6001 aagttcctgg cggtgattgt ggaggccagt ggagggggtg cctttctggt gctcccccta
     6061 agcaagacgg gccgcattga caaggcctac ccgacggtgt gtgggcacac gggacctgtc
     6121 ctggacatcg actggtgtcc tcacaacgac gaagtcatag ccagcggctc ggaggactgc
     6181 acggtcatgg tgtggcagat
//
