LOCUS       sequence-426141-        6371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human DCAF11. N-terminal 6X His tag
            with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_DCAF11:1-546
FEATURES             Location/Qualifiers
     source          1..6371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(700..705,706..722,723..728))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1080..1171
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1096..1113
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1230..1247
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1260..1280
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(2131..2145)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     CDS             complement(2538..2549)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    3056..3190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3093..3112)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3147..3166
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3219..3384
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3568..4022
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3655..3674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3864..3885
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4048..4152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4153..4221,4222..5013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4371..4390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5184..5772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5673..5692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5926..5943
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6077..6301
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(6368..6371,1..530))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta
       61 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca
      121 agcgcgttgg cctcatgctt gagcagattg atgagcgcgg tggcaatgcc ctgcctccgg
      181 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg
      241 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
      301 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
      361 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
      421 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
      481 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
      541 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag
      601 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
      661 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct
      721 tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg
      781 ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc
      841 tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg
      901 gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt gccggtggtg
      961 ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc
     1021 gcccaggact ctagctatag ttctagtggt tggctacgta tactccggaa tattaataga
     1081 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     1141 taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc gtcccaccat
     1201 cgggcgcgga tctcggtccg aaaaccatgc atcatcatca tcatcacagc agcggcagag
     1261 aaaacttgta tttccagggc atgggatcgc ggaacagcag cagtgcagga tccgggtccg
     1321 gagacccctc cgagggcttg ccccgaagag gggctggcct gcgtcggagt gaggaagagg
     1381 aagaagagga tgaagatgtg gatctggccc aggtactggc ctatctcctc cgcagaggcc
     1441 aagtgaggtt ggtgcaggga ggaggtgcag caaatttaca attcattcag gccctcttgg
     1501 actcagagga agagaatgac agagcttggg atggtcgtct tggggatcga tacaacccac
     1561 ctgtggatgc tacccctgac acccgggagc tggaattcaa tgagatcaag acacaagtgg
     1621 aactggccac agggcagctg gggcttaggc gggccgccca gaagcacagc tttcctcgaa
     1681 tgttgcacca gagagaacgg ggcctctgcc atcggggaag cttctccctt ggagaacagt
     1741 ctcgagtgat atctcacttc ttgcccaatg atctgggctt cactgatagc tactctcaga
     1801 aggctttctg tggcatctac agcaaagatg gtcaaatatt catgtctgct tgccaagacc
     1861 agacaatccg actctatgac tgccgatatg gccgtttcca taaattcaag agcatcaagg
     1921 cccgcgatgt aggctggagc gtcttggatg tggccttcac ccctgatggg aaccacttcc
     1981 tctactctag ctggtctgat tacattcata tctgcaatat ctatggtgag ggagatacac
     2041 acactgccct ggatctcagg ccagatgagc gtcgctttgc tgtcttctcc attgctgtct
     2101 cctcagatgg acgagaagta ctaggagggg ccaatgatgg ctgcctgtat gtctttgacc
     2161 gagaacagaa ccggcgcacc cttcagattg agtcccatga ggatgatgtg aatgcagtgg
     2221 cctttgctga tataagctcc caaatcctgt tctctggggg agatgatgcc atctgcaaag
     2281 tgtgggatcg acgcaccatg cgggaggatg accccaagcc tgtgggtgca ctggctggac
     2341 accaggatgg catcaccttc attgacagca agggtgatgc ccggtatctg atctccaact
     2401 ctaaagacca gaccatcaaa ctctgggata tccgacgctt ttccagccgg gaaggcatgg
     2461 aagcttcacg ccaggctgcc acacagcaaa actgggacta tcggtggcag caagtgccca
     2521 aaaaagcctg gcggaagctg aagctcccag gggacagctc cttgatgacc taccggggcc
     2581 acggagtgct gcacaccctc atccgctgcc ggttctcccc cattcatagc actggccagc
     2641 agttcatcta cagtggctgc tccactggca aagtggttgt gtacgacctt ctaagtggcc
     2701 acattgtgaa gaagctgacc aaccacaagg cctgtgtgcg tgacgtcagt tggcacccct
     2761 ttgaagagaa gattgtcagc agttcgtggg acgggaacct gcgtctgtgg cagtaccgcc
     2821 aggctgagta cttccaggat gacatgccag aatctgagga atgtgccagc gcccctgccc
     2881 cagtgcccca atcctctaca cccttttcct caccccagtg atgacgaagc ttgtcgagaa
     2941 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     3001 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3061 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3121 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3181 tgtctggatc tgatcactga tatcgcctag gagatccgaa ccagataagt gaaatctagt
     3241 tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca cccagttccc
     3301 atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac ccctcccagt
     3361 tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt ttttaatcaa
     3421 acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct ctgtcacaga
     3481 atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga atatcaacgc
     3541 ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     3601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3721 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3781 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3841 cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa cactcaaccc
     3901 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3961 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     4021 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4081 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4141 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4201 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4261 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4321 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4381 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4441 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4501 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4561 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4621 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4681 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4741 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4801 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4861 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4921 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4981 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5041 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5101 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5521 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5581 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5641 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5701 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5761 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5821 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5881 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5941 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     6001 caccgcatag accagccgcg taacctggca aaatcggtta cggttgagta ataaatggat
     6061 gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa atagatctaa
     6121 actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc agtccagtta
     6181 tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc attttctgaa
     6241 gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatggtaa agactatatt
     6301 cgcggcgttg tgacaattta ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa
     6361 cgaattgtta g
//
