LOCUS       sequence-426145-        5642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human DCAF13 fragment F54 to A356. N-terminal 
            6X His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_DCAF13:54-356
FEATURES             Location/Qualifiers
     source          1..5642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  70..294
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(361..894)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1064..1069,1070..1086,1087..1092))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1444..1535
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1460..1477
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1594..1611
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1624..1644
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2691..2825
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2728..2747)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2782..2801
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2854..3019
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3203..3657
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3290..3309)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3499..3520
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3683..3787
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3788..3856,3857..4648)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4006..4025)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4819..5407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5308..5327
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5561..5578
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tagaccagcc gcgtaacctg gcaaaatcgg ttacggttga gtaataaatg gatgccctgc
       61 gtaagcgggt gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga
      121 caataaagtc ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg
      181 aaaaagcata ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa
      241 ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg taaagactat attcgcggcg
      301 ttgtgacaat ttaccgaaca actccgcggc cgggaagccg atctcggctt gaacgaattg
      361 ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt
      421 tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc
      481 accaagcgcg ttggcctcat gcttgagcag attgatgagc gcggtggcaa tgccctgcct
      541 ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa
      601 cttgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag
      661 cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg
      721 ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga
      781 tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa
      841 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
      901 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
      961 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     1021 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     1081 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     1141 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     1201 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     1261 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     1321 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     1381 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     1441 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     1501 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     1561 ccatcgggcg cggatctcgg tccgaaaacc atgcatcatc atcatcatca cagcagcggc
     1621 agagaaaact tgtatttcca gggctttgca aaaccattcc ttgcttcgct ggatggtcac
     1681 cgtgatggag tcaattgctt ggcaaagcat ccagagaagc tggctactgt cctttctggg
     1741 tcgtgtgatg gagaggttag aatttggaat ctaactcagc ggaattgtat ccgtacaata
     1801 caagcacatg aaggctttgt acgaggaata tgtactcgct tttgtgggac ttcttttttc
     1861 actgttggtg atgacaaaac tgtgaagcag tggaaaatgg atgggccagg ctatggagac
     1921 gaggaagagc cattacatac aatattagga aagacagtgt atactgggat tgatcatcac
     1981 tggaaagaag ctgtttttgc cacatgtgga cagcaagtag acatttggga tgaacaaaga
     2041 actaatccta tatgttcaat gacctgggga tttgacagta taagtagtgt taaatttaac
     2101 ccaattgaga catttctctt gggaagttgt gcatctgaca ggaatatagt actgtacgat
     2161 atgaggcaag ctactccttt gaaaaaggtt atcttagata tgagaacaaa tacaatctgt
     2221 tggaacccta tggaagcttt catttttaca gcagcaaatg aagattataa cttatatact
     2281 tttgatatgc gtgcactgga cactcctgta atggtccata tggatcatgt atctgcagtg
     2341 cttgatgtgg attactctcc cactgggaag gagtttgtgt ctgctagttt cgataaatct
     2401 attcgaatct ttcctgtaga caaaagtcga agcagggagg tatatcatac aaagagaatg
     2461 caacatgtta tctgtgtaaa atggacttct gacagcaagt atattatgtg tggatctgat
     2521 gaaatgaaca ttcgcctgtg gaaagctaat gcttgatgac gaagcttgtc gagaagtact
     2581 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     2641 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     2701 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2761 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     2821 ggatctgatc actgatatcg cctaggagat ccgaaccaga taagtgaaat ctagttccaa
     2881 actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta
     2941 ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca
     3001 actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta atcaaacatc
     3061 ctgccaactc catgtgacaa accgtcatct tcggctactt tttctctgtc acagaatgaa
     3121 aatttttctg tcatctcttc gttattaatg tttgtaattg actgaatatc aacgcttatt
     3181 tgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3241 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     3301 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3361 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     3421 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3481 gagtccacgt tcttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3541 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3601 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     3661 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3721 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3781 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     3841 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     3901 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     3961 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4021 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4081 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4141 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4201 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4261 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4321 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4381 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4441 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4501 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4561 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4621 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     4681 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4741 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4801 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4861 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4921 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     4981 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5041 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5101 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5161 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5221 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5281 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5341 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5401 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5461 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     5521 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     5581 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     5641 ca
//
