LOCUS       sequence-426147-        5708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human EIF3I. N-terminal 6X His tag 
            with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_EIF3I:1-325
FEATURES             Location/Qualifiers
     source          1..5708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  37..261
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(328..861)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1031..1036,1037..1053,1054..1059))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1411..1502
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1427..1444
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1561..1578
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1591..1611
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2724..2858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2761..2780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2815..2834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2887..3052
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3236..3690
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3323..3342)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3532..3553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3716..3820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3821..3889,3890..4681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4039..4058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4852..5440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5341..5360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5594..5611
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggttgagta ataaatggat gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa
       61 actgaacaaa atagatctaa actatgacaa taaagtctta aactagacag aatagttgta
      121 aactgaaatc agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag
      181 caaactcttc attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggccaag
      241 ggcatggtaa agactatatt cgcggcgttg tgacaattta ccgaacaact ccgcggccgg
      301 gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg
      361 catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta
      421 atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct tgagcagatt
      481 gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatagat
      541 atagatctca ctacgcggct gctcaaactt gggcagaacg taagccgcga gagcgccaac
      601 aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga gcaagttccc
      661 gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga actcacgacc
      721 gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc tacatgtgcg
      781 aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct gctgcgtaac
      841 atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga
      901 tgcccgaggc atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
      961 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta
     1021 cttgcattac agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg
     1081 tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc
     1141 ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt
     1201 gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag
     1261 acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct
     1321 cggttttctg gaaggcgagc atcgtttgtt cgcccaggac tctagctata gttctagtgg
     1381 ttggctacgt atactccgga atattaatag atcatggaga taattaaaat gataaccatc
     1441 tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa
     1501 atattccgga ttattcatac cgtcccacca tcgggcgcgg atctcggtcc gaaaaccatg
     1561 catcatcatc atcatcacag cagcggcaga gaaaacttgt atttccaggg catgaagccg
     1621 atcctactgc agggccatga gcggtccatt acgcagatta agtataaccg cgaaggagac
     1681 ctcctcttta ctgtggccaa ggaccctatc gtcaatgtat ggtactctgt gaatggtgag
     1741 aggctgggca cctacatggg ccataccgga gctgtgtggt gtgtggacgc tgactgggac
     1801 accaagcatg tcctcactgg ctcagctgac aacagctgtc gtctctggga ctgtgaaaca
     1861 ggaaagcagc tggcccttct caagaccaat tcggctgtcc ggacctgcgg ttttgacttt
     1921 gggggcaaca tcatcatgtt ctccacggac aagcagatgg gctaccagtg ctttgtgagc
     1981 ttttttgacc tgcgggatcc gagccagatt gacaacaatg agccctacat gaagatccct
     2041 tgcaatgact ctaaaatcac cagtgctgtt tggggacccc tgggggagtg catcatcgct
     2101 ggccatgaga gtggagagct caaccagtat agtgccaagt ctggagaggt gttggtgaat
     2161 gttaaggagc actcccggca gatcaacgac atccagttat ccagggacat gaccatgttt
     2221 gtgaccgcgt ccaaggacaa cacagccaag ctttttgact ccacaactct tgaacatcag
     2281 aagactttcc ggacagaacg tcctgtcaac tcagctgccc tctcccccaa ctatgaccat
     2341 gtggtcctgg gcggtggtca ggaagccatg gatgtaacca caacctccac caggattggc
     2401 aagtttgagg ccaggttctt ccatttggcc tttgaagaag agtttggaag agtcaagggt
     2461 cactttggac ctatcaacag tgttgccttc catcctgatg gcaagagcta cagcagcggc
     2521 ggcgaagatg gttacgtccg tatccattac ttcgacccac agtacttcga atttgagttt
     2581 gaggcttgat gacgaagctt gtcgagaagt actagaggat cataatcagc cataccacat
     2641 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2701 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2761 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2821 tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgata tcgcctagga
     2881 gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt aattttcgta
     2941 ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct
     3001 taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca
     3061 tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga caaaccgtca
     3121 tcttcggcta ctttttctct gtcacagaat gaaaattttt ctgtcatctc ttcgttatta
     3181 atgtttgtaa ttgactgaat atcaacgctt atttgcagcc tgaatggcga atgggacgcg
     3241 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3301 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3361 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3421 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3481 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttcttaat agtggactct
     3541 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3601 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3661 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     3721 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3781 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3841 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3901 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3961 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4021 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4081 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4141 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4201 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4261 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4321 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4381 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4441 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4501 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4561 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4621 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4681 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4741 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4801 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4861 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4921 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4981 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5041 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5101 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5161 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5221 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5281 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5341 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5401 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5461 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5521 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5581 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     5641 tctccttacg catctgtgcg gtatttcaca ccgcatagac cagccgcgta acctggcaaa
     5701 atcggtta
//
