LOCUS       sequence-426149-        5789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human CSTF1 fragment G80 to D431. N-terminal 6X
            His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_CSTF1:80-431
FEATURES             Location/Qualifiers
     source          1..5789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..482
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(115..134)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     324..345
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        508..612
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(613..681,682..1473)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(831..850)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1644..2232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2133..2152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2386..2403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2537..2761
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2828..3361)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3531..3536,3537..3553,3554..3559))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3911..4002
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3927..3944
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4061..4078
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4091..4111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    5305..5439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5342..5361)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5396..5415
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5468..5633
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
       61 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      121 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      181 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      241 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
      301 cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa cactcaaccc
      361 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
      421 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
      481 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      541 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      601 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      661 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      721 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
      781 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      841 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      901 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      961 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1021 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1081 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1141 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1201 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1261 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     1321 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     1381 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1441 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1501 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1561 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1621 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1681 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1741 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1801 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1861 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1921 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1981 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2041 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2101 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2161 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2221 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2281 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2341 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2401 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     2461 caccgcatag accagccgcg taacctggca aaatcggtta cggttgagta ataaatggat
     2521 gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa atagatctaa
     2581 actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc agtccagtta
     2641 tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc attttctgaa
     2701 gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatggtaa agactatatt
     2761 cgcggcgttg tgacaattta ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa
     2821 cgaattgtta ggtggcggta cttgggtcga tatcaaagtg catcacttct tcccgtatgc
     2881 ccaactttgt atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca
     2941 cataagcacc aagcgcgttg gcctcatgct tgagcagatt gatgagcgcg gtggcaatgc
     3001 cctgcctccg gtgctcgccg gagactgcga gatcatagat atagatctca ctacgcggct
     3061 gctcaaactt gggcagaacg taagccgcga gagcgccaac aaccgcttct tggtcgaagg
     3121 cagcaagcgc gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct
     3181 gatgttggga gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc
     3241 gcatggattt gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc
     3301 cacctaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca
     3361 tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta
     3421 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
     3481 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa
     3541 ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc
     3601 cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca
     3661 aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg
     3721 tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct
     3781 tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc
     3841 atcgtttgtt cgcccaggac tctagctata gttctagtgg ttggctacgt atactccgga
     3901 atattaatag atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt
     3961 tactgttttc gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac
     4021 cgtcccacca tcgggcgcgg atctcggtcc gaaaaccatg catcatcatc atcatcacag
     4081 cagcggcaga gaaaacttgt atttccaggg cgggattgac ctggaatttg atgcagatgt
     4141 tcagactatg tccccagagg cttctgagta cgaaacatgc tatgtcacat cacataaagg
     4201 accatgccgt gtagctacct atagtagaga tggacagtta atagctactg ggtctgctga
     4261 tgcttcgata aagatacttg acacagagag gatgttggcc aaaagtgcca tgccaataga
     4321 ggtcatgatg aatgagaccg cacaacaaaa tatggaaaac cacccagtga ttcgaactct
     4381 ttatgaccat gtggatgaag tcacgtgcct tgctttccac ccaacagaac agatcctggc
     4441 ttctggttca agggattata ctcttaaatt atttgattat tccaaaccat cagcaaaaag
     4501 agccttcaaa tacattcagg aagctgaaat gttacgttcc atctcttttc atccttctgg
     4561 agactttata cttgtcggaa ctcagcatcc tactcttcgc ctttatgata tcaacacctt
     4621 tcaatgtttt gtctcttgca atcctcaaga tcaacacacc gatgctatat gttccgttaa
     4681 ttacaattct agtgccaata tgtacgtaac tggaagcaag gacggctgca tcaaattatg
     4741 ggatggtgtt tcaaatcgat gcatcacaac ttttgagaaa gcacatgacg gtgctgaagt
     4801 ttgttctgcc attttttcca aaaattctaa atacattctc tcaagtggaa aagactctgt
     4861 agctaaactt tgggaaatat caacgggacg aacactggtc agatacacgg gcgcgggttt
     4921 aagtggacgc caggtgcacc ggacacaggc tgtgtttaac cacaccgagg actatgtgtt
     4981 gctgcccgac gagaggacga tcagtctttg ctgctgggac tcgaggacag ccgagcggag
     5041 aaacctgctg tcgttggggc acaacaatat tgtacgctgc atagtgcact cccccaccaa
     5101 ccccgggttc atgacgtgca gcgatgactt cagagcgcgg ttttggtacc ggagatcgac
     5161 cactgactga tgacgaagct tgtcgagaag tactagagga tcataatcag ccataccaca
     5221 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     5281 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     5341 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5401 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgat atcgcctagg
     5461 agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
     5521 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
     5581 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc
     5641 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
     5701 atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt
     5761 aatgtttgta attgactgaa tatcaacgc
//
