LOCUS       sequence-426150-        5765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human DCAF8L2 fragment G207 to L550. N-terminal
            6X His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_DCAF8L2:207-550
FEATURES             Location/Qualifiers
     source          1..5765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    750..884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(787..806)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     841..860
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  913..1078
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1262..1716
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1349..1368)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1558..1579
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1742..1846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1847..1915,1916..2707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2065..2084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2878..3466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3367..3386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3620..3637
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3771..3995
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4062..4595)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4765..4770,4771..4787,4788..4793))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5145..5236
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5161..5178
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5295..5312
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5325..5345
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 caagaccggc cagcttcaaa agttgtggta acaagagaaa atgataagaa agtgggactg
       61 tatacaatta ctgtgaatcc cgccaatacc taccaatttg cagtgggtgg acaagatcag
      121 tttgtaagga tttatgacca gaggaaaatt gataagaaag aaaacaatgg cgtgctcaag
      181 aaattcactc ctcatcatct ggttaattgt gatttcccaa caaacatcac ctgcgttgtg
      241 tacagccacg atggcacaga gctgctagcc agctacaatg atgacgatat ttacctcttc
      301 aactcctctc acagtgatgg tgctcaatac agtaagagat ttaagggaca cagaaataat
      361 accacagtca aaggtgttaa tttctatggc cccaggagtg agtttgtagt gagcggtagt
      421 gattgcgggc acatcttctt ctgggagaaa tcatcctgcc aaatcatcca gttcctaaag
      481 gggagcagag aaggtacaat aaactgtctt gaaccccacc cttacctacc tgtgttggcg
      541 tgcagtggcc tagatcatga tgtcaagatc tggacaccca cagctaaagc tgccactgag
      601 cttactgggt tatgatgacg aagcttgtcg agaagtacta gaggatcata atcagccata
      661 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      721 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      781 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      841 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca ctgatatcgc
      901 ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt
      961 ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat
     1021 aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc
     1081 ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa
     1141 ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt catctcttcg
     1201 ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa tggcgaatgg
     1261 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     1321 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     1381 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     1441 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     1501 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg
     1561 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     1621 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1681 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     1741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     2101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2221 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2401 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2461 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2701 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2761 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3601 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     3661 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct
     3721 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     3781 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     3841 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     3901 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     3961 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     4021 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     4081 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     4141 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     4201 tgcttgagca gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     4261 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     4321 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     4381 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     4441 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     4501 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     4561 ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
     4621 gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg
     4681 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     4741 gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt
     4801 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc
     4861 gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag
     4921 gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt
     4981 caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa
     5041 gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca ggactctagc
     5101 tatagttcta gtggttggct acgtatactc cggaatatta atagatcatg gagataatta
     5161 aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca gttttgtaat
     5221 aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc gcggatctcg
     5281 gtccgaaaac catgcatcat catcatcatc acagcagcgg cagagaaaac ttgtatttcc
     5341 agggcggggc aagagccttt gtgcagcgtt tccgcctgca atatcgtctt gcagaccatg
     5401 tcggctgtgt caatactgta cactttaacc agcgtggcac ccggctggcc agtagcggtg
     5461 atgacctaaa ggtgatagtg tgggactggg tgcggcagag gccagtactg aactttgaaa
     5521 gtggtcacac aaataatgtc ttccaggcca agttccttcc taactgtggt gattccactc
     5581 tggccatgtg tgcccgtgat gggcaggtac gggtagcaga gttaattaat gcatcatatt
     5641 tcaacaatac taagtgtgtg gcccagcaca ggggacctgc ccacaagttg gctctggagc
     5701 ctgactctcc ttataagttc ctcacttcag gtgaagatgc tgttgtcttc accattgacc
     5761 tcaga
//
