LOCUS       sequence-426153-        6791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human UTP4. N-terminal 6X His tag 
            with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_UTP4:1-686
FEATURES             Location/Qualifiers
     source          1..6791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    679..813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(716..735)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     770..789
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  842..1007
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1191..1645
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1278..1297)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1487..1508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1671..1775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1776..1844,1845..2636)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1994..2013)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2807..3395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3296..3315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3549..3566
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3700..3924
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3991..4524)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4694..4699,4700..4716,4717..4722))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5074..5165
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5090..5107
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5224..5241
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5254..5274
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tggagtccat gtctacaacg taaaacagct aaagcttcac tgcacggtgc ctgcttacaa
       61 tttcccagtg actgctatgg ctattgcccc caataccaac aaccttgtca tcgctcattc
      121 ggaccagcag gtatttgagt acagcatccc agacaaacag tatacagatt ggagccggac
      181 tgtccagaag cagggctttc accacctttg gctccaaagg gatactccta tcacacacat
      241 cagttttcat cccaagagac cgatgcacat ccttctccat gatgcctaca tgttctgcat
      301 cattgacaag tcattgcccc ttccaaatga caaaacctta ctctacaatc catttcctcc
      361 cacgaatgaa tcagatgtca tccggaggcg cacagctcat gcttttaaaa tttctaagat
      421 atataagcct ctactcttca tggatctttt ggatgaaaga acactcgtgg cagtagaacg
      481 gcctctggat gacatcattg ctcagctccc accacccatt aaaaagaaga aatttggaac
      541 ctgatgacga agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta
      601 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
      661 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      721 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      781 aaactcatca atgtatctta tcatgtctgg atctgatcac tgatatcgcc taggagatcc
      841 gaaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc
      901 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa
      961 actccatttc cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt
     1021 cttcctgtta tgtttttaat caaacatcct gccaactcca tgtgacaaac cgtcatcttc
     1081 ggctactttt tctctgtcac agaatgaaaa tttttctgtc atctcttcgt tattaatgtt
     1141 tgtaattgac tgaatatcaa cgcttatttg cagcctgaat ggcgaatggg acgcgccctg
     1201 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1261 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1321 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1381 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     1441 atagacggtt tttcgccctt tgacgttgga gtccacgttc ttaatagtgg actcttgttc
     1501 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     1561 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     1621 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1681 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1741 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1801 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1861 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1921 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1981 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2041 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2101 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2161 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2221 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2281 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2341 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2401 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2461 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2521 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2581 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2641 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2701 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2761 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2821 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2881 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2941 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3001 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3061 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3121 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3181 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3241 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3361 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3421 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3481 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3541 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     3601 ttacgcatct gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg gcaaaatcgg
     3661 ttacggttga gtaataaatg gatgccctgc gtaagcgggt gtgggcggac aataaagtct
     3721 taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga cagaatagtt
     3781 gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta
     3841 aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc
     3901 aagggcatgg taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc
     3961 cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa
     4021 gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc
     4081 gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgagcag
     4141 attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata
     4201 gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc
     4261 aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt
     4321 cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg
     4381 accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt
     4441 gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt
     4501 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     4561 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac
     4621 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
     4681 ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat
     4741 ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct
     4801 gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc
     4861 cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg
     4921 aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat
     4981 cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag
     5041 tggttggcta cgtatactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     5101 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     5161 taaatattcc ggattattca taccgtccca ccatcgggcg cggatctcgg tccgaaaacc
     5221 atgcatcatc atcatcatca cagcagcggc agagaaaact tgtatttcca gggcatgggt
     5281 gaatttaagg tccatcgagt acgtttcttt aattatgttc catcaggaat ccgctgtgtg
     5341 gcttacaata accagtcaaa cagattggct gtttcacgaa cagatggcac tgtggaaatt
     5401 tataacttgt cagcaaacta ctttcaggag aaatttttcc caggtcatga gtctcgggct
     5461 acagaagctt tgtgctgggc agaaggacag cgactcttta gtgctgggct caatggcgag
     5521 attatggagt atgatttaca ggcgttaaac atcaagtatg ctatggatgc ctttggagga
     5581 cctatttgga gcatggctgc cagccccagt ggctctcaac ttttggttgg ttgtgaagat
     5641 ggatctgtga aactatttca aattacccca gacaaaatcc agtttgaaag aaattttgat
     5701 cggcagaaaa gtcgcatcct gagtctcagc tggcatccct ctggtaccca cattgcagct
     5761 ggttccatag actacattag tgtgtttgat gtcaaatcag gcagcgctgt tcataagatg
     5821 attgtggaca ggcagtatat gggcgtgtct aagcggaagt gcatcgtgtg gggtgtcgcc
     5881 ttcttgtccg atggcactat cataagtgtg gactctgctg ggaaggtgca gttctgggac
     5941 tcagccactg ggacgcttgt gaagagccat ctcatcgcta atgctgacgt gcagtccatt
     6001 gctgtagctg accaagaaga cagtttcgtg gtgggcacag ccgagggaac agtcttccat
     6061 tttcagctgg tccctgtgac atctaacagc agtgagaagc agtgggtgcg gacaaaaccg
     6121 ttccagcatc acactcatga cgtgcgcact gtggcccaca gcccaacagc gctgatatct
     6181 ggaggcactg acacccactt agtctttcgt cctctcatgg agaaggtgga agtaaagaat
     6241 tacgatgccg ctctccgaaa aatcaccttt ccccaccgat gtctcatctc ctgttctaaa
     6301 aagaggcagc ttctcctctt ccagtttgct catcacttag aactttggcg actgggatcc
     6361 acagttgcaa caggcaagaa tggggatact cttccactct ctaaaaatgc agatcattta
     6421 ctgcacctaa agacaaaggg tcctgagaac attatctgta gctgtatctc cccatgtgga
     6481 agttggatag cctattctac agtttctcgg ttttttctct atcggctgaa ttatgaacat
     6541 gacaacataa gcctcaaaag ggtttccaaa atgccagcat tccttcgctc tgcccttcag
     6601 attttgtttt ctgaagattc aacaaagctc tttgtagcat caaatcaagg agctctgcat
     6661 attgttcagc tgtcaggagg aagcttcaag cacctgcatg ctttccagcc tcagtcagga
     6721 acagtggagg ccatgtgtct tttggcagtc agtccagatg ggaattggct agctgcatca
     6781 ggtaccagtg c
//
