LOCUS       sequence-426155-        5894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human WDR89. N-terminal 6X His tag 
            with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_WDR89:1-387
FEATURES             Location/Qualifiers
     source          1..5894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    815..949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(852..871)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     906..925
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  978..1143
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1327..1781
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1414..1433)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1623..1644
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1807..1911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1912..1980,1981..2772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2130..2149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2943..3531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3432..3451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3685..3702
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3836..4060
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4127..4660)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4830..4835,4836..4852,4853..4858))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5210..5301
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5226..5243
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5360..5377
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5390..5410
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttggtgcata ttcagagaca catagtgatg atgtcactca agtacgtttc catcccagca
       61 atcccaacat ggtagtctca ggttcatctg atggcctggt aaatgtattt gatattaata
      121 ttgataatga ggaggatgca ctggttacaa cctgtaactc aatttcatca gtaagctgta
      181 ttggttggtc tgggaaaggt tataaacaga tttactgcat gacacatgat gaaggatttt
      241 attggtggga tcttaatcat ctggacactg atgaaccagt tacacgtttg aacatccagg
      301 atgtcagaga agtagttaac atgaaagaag atgctttgga ctatttgatt ggtggcctat
      361 atcatgaaaa gacagacaca ttgcatgtta ttggaggaac aaacaaagga aggattcatt
      421 tgatgaactg cagcatgtca ggactgaccc atgtgactag ccttcaggga gggcatgctg
      481 ctacagtccg ttctttctgt tggaatgtgc aagatgattc tttgttgact ggaggagaag
      541 atgcacagtt gttactttgg aaacctggag ctatagagaa gacctttaca aagaaagaga
      601 gtatgaaaat agcatcctct gtgcaccaac gagtacgagt tcatagtaat gattcttata
      661 aaagaaggaa aaagcagtga tgacgaagct tgtcgagaag tactagagga tcataatcag
      721 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      781 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
      841 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      901 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgat
      961 atcgcctagg agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     1021 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     1081 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     1141 acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg
     1201 acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct
     1261 cttcgttatt aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg
     1321 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1381 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     1441 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     1501 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     1561 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttaa
     1621 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     1681 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     1741 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     1801 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1861 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1921 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1981 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2041 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2101 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2161 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2221 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2281 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2341 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2401 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2461 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2521 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2581 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2641 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2701 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2761 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2821 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2881 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2941 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3001 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3061 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3121 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3181 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3241 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3301 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3361 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3421 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3481 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3541 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3601 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3661 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     3721 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga ccagccgcgt
     3781 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg
     3841 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa
     3901 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg
     3961 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt
     4021 aaagaggggc gtggccaagg gcatggtaaa gactatattc gcggcgttgt gacaatttac
     4081 cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac
     4141 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
     4201 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
     4261 cctcatgctt gagcagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg
     4321 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt
     4381 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct
     4441 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
     4501 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
     4561 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
     4621 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     4681 aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag tcataacaag
     4741 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
     4801 tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact
     4861 gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg
     4921 aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc tccacgcatc
     4981 gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg gatctgccct
     5041 ggcttcagga gatcggaaga cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg
     5101 atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc gcccaggact
     5161 ctagctatag ttctagtggt tggctacgta tactccggaa tattaataga tcatggagat
     5221 aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt
     5281 gtaataaaaa aacctataaa tattccggat tattcatacc gtcccaccat cgggcgcgga
     5341 tctcggtccg aaaaccatgc atcatcatca tcatcacagc agcggcagag aaaacttgta
     5401 tttccagggc atggaaaaga ttgaggaaca atttgctaat ctgcacattg ttaaatgttc
     5461 cttaggaacc aaagagccca cttaccttct tggtatagac acatcaaaga ctgtccaagc
     5521 aggaaaggaa aacttggttg ctgttttatg ttctaatgga tcaatcagaa tatatgataa
     5581 agaaaggtta aatgtactac gagaatttag tggatatcct ggacttctta atggagtcag
     5641 atttgcaaat tcctgtgaca gtgtatattc agcatgtact gatggcactg tgaaatgctg
     5701 ggatgctcga gtagccagag aaaaacctgt tcagctcttc aagggttacc cttccaatat
     5761 ttttatcagt tttgatatta attgtaatga tcatattatt tgtgctggta cagaaaaagt
     5821 tgatgatgat gcattgttgg tgttttggga tgcaaggatg aattctcaga atttatctac
     5881 aactaaagac tcac
//
