LOCUS       sequence-426175-        3695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning A. thaliana HTR5 promoter as GreenGate module E1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M,
            Dolan L, Mittelsten Scheid O
  TITLE     A versatile CRISPR-based system for lineage tracing in living 
            plants.
  JOURNAL   Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378.
  PUBMED    37403571
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2023 Jul 5. doi: 10.1111/tpj.16378."
COMMENT     Sequence Label: pGGE1-HTR5p
FEATURES             Location/Qualifiers
     source          1..3695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1230..1248)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1231..1248)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1792..1809)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1963..2551)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2043..2062)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2722..3513,3514..3582))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3345..3364
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3583..3686)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatacgact cactataggg agacgcacat gcggccgcct cgagaattct gacgtcttaa
       61 ttaattatta tcagggtggt ggtctcactg ctttctgccc ttgccaatcc atatctgaag
      121 aagaaaaaaa aacacaaaat tacgatttag atccgatata acaaaatttg aatcgcacag
      181 atcgatctct ttggagattc tatacctaga aaatggagac gattttcaaa tctctgtaaa
      241 aattctggtt tcttcttgac ggaagaagac gacgactcca atatttcggt tagtactgaa
      301 ccggaaagtt tgactggtgc aaccaattta atgtaccgta cgtaacgcac caatcggatt
      361 ttgtattcaa tgggccttat ctgtgagccc attaattgat gtgacggcct aaactaaatc
      421 cgaacggttt atttcagcga tccgcgacgg tttgtattca gccaatagca atcaattatg
      481 tagcagtggt gatcctcgtc aaaccagtaa agctagatct ggaccgttga attggtgcaa
      541 gaaagcacat gttgtgatat ttttacccgt acgattagaa aacttgagaa acacattgat
      601 aatcgataaa aaccgtccga tcatataaat ccgctttacc atcgttgcct ataaattaat
      661 atcaatagcc gtacacgcgt gaagactgac aatattatct ttttcgaatt cggagctcaa
      721 gtttgaaatt cggagaagct agagagtttt ctgaggtacg attcttcgat cctctttgat
      781 tttcctggaa atattttttc ggtgatcgtg aaactactgg aatcgctcga taggtggtac
      841 gaaattaggc gagattagtt tctattcttg gccattatct tgtttcttcg ccgaatgatc
      901 ttccggataa agattttagg ttagagatga atcgtatagc tagatttcat caccagatag
      961 tttctttgtc tagaatctct gaaattctcg atagttttca catgtgtaaa tagattgttc
     1021 ttattcggcg attgttgatt agggttttga ttttcttgat tatgcgattg caattaggga
     1081 ttttctttgg ttttgtgttg atcttacgat acattccggc aattgaatac gtatggatct
     1141 aaatcttgtt aatttgttga acaggacgcg agaccggtgg tatcatggtt tttacctcct
     1201 gaattcggat ccctcgagcg atacacactt ctatagtgtc acctaaatgc gtttaaacct
     1261 tcctgcaggt gacgattacc taacaatcgg tcgattcgtt tgatgttatg ttttgttctc
     1321 gctttggttg gcaggttacg gccaagttcg gtaagagtga gagttttaca gtcaagtaat
     1381 gcgtggcaag ccaacgttaa gctgttgagt cgttttaagt gtaattcggg gcagaattgg
     1441 taaagagagt cgtgtaaaat atcgagttcg cacatcttgt tgtctgatta ttgatttttc
     1501 gcgaaaccat ttaatcatat gacaagatgt gtgtccacct taacttaatg atttttacca
     1561 aaatcattag gggattcatc agcgctgagt gtgtaaatta atttttatgc cgcagcgggc
     1621 cagcaattct cgtgaatcat cgcttaaacg gcctgatttc tgaatagcta tgcgtaagcg
     1681 ggtgttgtct aacattctgc gttcctcttt atcctgtctg aaccggctgc attaatgaat
     1741 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1801 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1861 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1921 gcaaaaggcc aggaaccgta aaaaggccgc attgctggcg tttttccata ggctccgccc
     1981 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2041 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2101 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2161 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2221 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2281 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2341 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2401 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2461 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2521 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2581 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2641 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2701 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2761 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2821 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagatccac gctcaccggc
     2881 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2941 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3001 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3061 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3121 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3181 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3241 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3301 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3361 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3421 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3481 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3541 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3601 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3661 gaaaaataaa caaatagggg ttccgcccgc gaaat
//
