LOCUS       sequence-426184-        3111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning P. sativum PEA3A terminator as GreenGate module C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M,
            Dolan L, Mittelsten Scheid O
  TITLE     A versatile CRISPR-based system for lineage tracing in living 
            plants.
  JOURNAL   Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378.
  PUBMED    37403571
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2023 Jul 5. doi: 10.1111/tpj.16378."
COMMENT     Sequence Label: pGGC-PEA3AT
FEATURES             Location/Qualifiers
     source          1..3111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(645..664)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(646..664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        674..777
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(778..846,847..1638)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(996..1015)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1809..2397
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2298..2317
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2551..2568
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atttaggtga cactatagaa gtgtgtatcg ctcgagggat ccgaattcag gaggtaaaaa
       61 ccatgatggt ggtggtctcc ggctcacgac gaactctaag agctcaggcc tcccagcttt
      121 cgtccgtatc atcggtttcg acaacgttcg tcaagttcaa tgcatcagtt tcattgccca
      181 cacaccagaa tcctactaag tttgagtatt atggcattgg aaaagctgtt ttcttctatc
      241 atttgttctg cttgtaattt actgtgttct ttcagttttt gttttcggac atcaaaatgc
      301 aaatggatgg ataagagtta ataaatgata tggtcctttt gttcattctc aaattattat
      361 tatctgttgt ttttacttta atgggttgaa tttaagtaag aaaggaacta acagtgtgat
      421 attaaggtgc aatgttagac atataaaaca gtctttcacc tctctttggt tatgtcttga
      481 attggtttgt ttcttcactt atctgtgtaa tcaagtttac tatgagtcta tgatcaagta
      541 attatgcaat caagttaagt acagtatagg ctttcagcga gaccggtggt ctgataataa
      601 ttaattaaga cgtcagaatt ctcgaggcgg ccgcatgtgc gtctccctat agtgagtcgt
      661 attaatttcg cgggcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      721 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      781 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      841 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      901 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      961 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1021 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1081 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1141 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1201 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1261 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1321 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1381 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1441 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggatctcgc
     1501 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1561 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1621 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1681 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1741 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1801 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1861 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1921 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1981 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2041 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2101 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2161 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2221 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2281 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2341 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2401 gccagcaatg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2461 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2521 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2581 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagccggttc
     2641 agacaggata aagaggaacg cagaatgtta gacaacaccc gcttacgcat agctattcag
     2701 aaatcaggcc gtttaagcga tgattcacga gaattgctgg cccgctgcgg cataaaaatt
     2761 aatttacaca ctcagcgctg atgaatcccc taatgatttt ggtaaaaatc attaagttaa
     2821 ggtggacaca catcttgtca tatgattaaa tggtttcgcg aaaaatcaat aatcagacaa
     2881 caagatgtgc gaactcgata ttttacacga ctctctttac caattctgcc ccgaattaca
     2941 cttaaaacga ctcaacagct taacgttggc ttgccacgca ttacttgact gtaaaactct
     3001 cactcttacc gaacttggcc gtaacctgcc aaccaaagcg agaacaaaac ataacatcaa
     3061 acgaatcgac cgattgttag gtaatcgtca cctgcaggaa ggtttaaacg c
//
