LOCUS       sequence-426190-        8042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning estradiol-inducible expression cassette containi LexA XVE 
            binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate 
            module C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M,
            Dolan L, Mittelsten Scheid O
  TITLE     A versatile CRISPR-based system for lineage tracing in living 
            plants.
  JOURNAL   Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378.
  PUBMED    37403571
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2023 Jul 5. doi: 10.1111/tpj.16378."
COMMENT     Sequence Label: pGGC-LexACasPea
FEATURES             Location/Qualifiers
     source          1..8042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        438..484
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             506..4609
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             506..4609
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4622..4642
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(join(5570..5576,5577..5594,5595..5600))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    5615..5636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5756..5773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5927..6515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6007..6026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6686..7477,7478..7546))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7309..7328
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7547..7651)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7719..7737
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 taatacgact cactataggg agagcggccg ccagatcttc cggatggctc gagtttttca
       61 gcaagatggt ggtggtctcc ggctcctagc aaagtcgacg aattctcccg ggtggatcca
      121 aggccttcta gaaaaacgaa ttccatgcca gcttgggctg caggtcgagg ctaaaaaact
      181 aatcgcatta tcatcccctc gacgtactgt acatataacc actggtttta tatacagcag
      241 tactgtacat ataaccactg gttttatata cagcagtcga cgtactgtac atataaccac
      301 tggttttata tacagcagta ctgtacatat aaccactggt tttatataca gcagtcgagg
      361 taagattaga tatggatatg tatatggata tgtatatggt ggtaatgcca tgtaatatgc
      421 tcgactctag gatcttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      481 aggacacgct gaagctagtc gctgcatgga taagaagtac tctatcggac tcgatatcgg
      541 aactaactct gtgggatggg ctgtgatcac cgatgagtac aaggtgccat ctaagaagtt
      601 caaggttctc ggaaacaccg ataggcactc tatcaagaaa aaccttatcg gtgctctcct
      661 cttcgattct ggtgaaactg ctgaggctac cagactcaag agaaccgcta gaagaaggta
      721 caccagaaga aagaacagga tctgctacct ccaagagatc ttctctaacg agatggctaa
      781 agtggatgat tcattcttcc acaggctcga agagtcattc ctcgtggaag aagataagaa
      841 gcacgagagg caccctatct tcggaaacat cgttgatgag gtggcatacc acgagaagta
      901 ccctactatc taccacctca gaaagaagct cgttgattct actgataagg ctgatctcag
      961 gctcatctac ctcgctctcg ctcacatgat caagttcaga ggacacttcc tcatcgaggg
     1021 tgatctcaac cctgataact ctgatgtgga taagttgttc atccagctcg tgcagaccta
     1081 caaccagctt ttcgaagaga accctatcaa cgcttcaggt gtggatgcta aggctatcct
     1141 ctctgctagg ctctctaagt caagaaggct tgagaacctc attgctcagc tccctggtga
     1201 gaagaagaac ggacttttcg gaaacttgat cgctctctct ctcggactca cccctaactt
     1261 caagtctaac ttcgatctcg ctgaggatgc aaagctccag ctctcaaagg atacctacga
     1321 tgatgatctc gataacctcc tcgctcagat cggagatcag tacgctgatt tgttcctcgc
     1381 tgctaagaac ctctctgatg ctatcctcct cagtgatatc ctcagagtga acaccgagat
     1441 caccaaggct ccactctcag cttctatgat caagagatac gatgagcacc accaggatct
     1501 cacacttctc aaggctcttg ttagacagca gctcccagag aagtacaaag agattttctt
     1561 cgatcagtct aagaacggat acgctggtta catcgatggt ggtgcatctc aagaagagtt
     1621 ctacaagttc atcaagccta tcctcgagaa gatggatgga accgaggaac tcctcgtgaa
     1681 gctcaataga gaggatcttc tcagaaagca gaggaccttc gataacggat ctatccctca
     1741 tcagatccac ctcggagagt tgcacgctat ccttagaagg caagaggatt tctacccatt
     1801 cctcaaggat aacagggaaa agattgagaa gattctcacc ttcagaatcc cttactacgt
     1861 gggacctctc gctagaggaa actcaagatt cgcttggatg accagaaagt ctgaggaaac
     1921 catcacccct tggaacttcg aagaggtggt ggataagggt gctagtgctc agtctttcat
     1981 cgagaggatg accaacttcg ataagaacct tccaaacgag aaggtgctcc ctaagcactc
     2041 tttgctctac gagtacttca ccgtgtacaa cgagttgacc aaggttaagt acgtgaccga
     2101 gggaatgagg aagcctgctt ttttgtcagg tgagcaaaag aaggctatcg ttgatctctt
     2161 gttcaagacc aacagaaagg tgaccgtgaa gcagctcaaa gaggattact tcaagaaaat
     2221 cgagtgcttc gattcagttg agatttctgg tgttgaggat aggttcaacg catctctcgg
     2281 aacctaccac gatctcctca agatcattaa ggataaggat ttcttggata acgaggaaaa
     2341 cgaggatatc ttggaggata tcgttcttac cctcaccctc tttgaagata gagagatgat
     2401 tgaagaaagg ctcaagacct acgctcatct cttcgatgat aaggtgatga agcagttgaa
     2461 gagaagaaga tacactggtt ggggaaggct ctcaagaaag ctcattaacg gaatcaggga
     2521 taagcagtct ggaaagacaa tccttgattt cctcaagtct gatggattcg ctaacagaaa
     2581 cttcatgcag ctcatccacg atgattctct cacctttaaa gaggatatcc agaaggctca
     2641 ggtttcagga cagggtgata gtctccatga gcatatcgct aacctcgctg gatctcctgc
     2701 aatcaagaag ggaatcctcc agactgtgaa ggttgtggat gagttggtga aggtgatggg
     2761 aaggcataag cctgagaaca tcgtgatcga aatggctaga gagaaccaga ccactcagaa
     2821 gggacagaag aactctaggg aaaggatgaa gaggatcgag gaaggtatca aagagcttgg
     2881 atctcagatc ctcaaagagc accctgttga gaacactcag ctccagaatg agaagctcta
     2941 cctctactac ctccagaacg gaagggatat gtatgtggat caagagttgg atatcaacag
     3001 gctctctgat tacgatgttg atcatatcgt gccacagtca ttcttgaagg atgattctat
     3061 cgataacaag gtgctcacca ggtctgataa gaacaggggt aagagtgata acgtgccaag
     3121 tgaagaggtt gtgaagaaaa tgaagaacta ttggaggcag ctcctcaacg ctaagctcat
     3181 cactcagaga aagttcgata acttgactaa ggctgagagg ggaggactct ctgaattgga
     3241 taaggcagga ttcatcaaga ggcagcttgt ggaaaccagg cagatcacta agcacgttgc
     3301 acagatcctc gattctagga tgaacaccaa gtacgatgag aacgataagt tgatcaggga
     3361 agtgaaggtt atcaccctca agtcaaagct cgtgtctgat ttcagaaagg atttccaatt
     3421 ctacaaggtg agggaaatca acaactacca ccacgctcac gatgcttacc ttaacgctgt
     3481 tgttggaacc gctctcatca agaagtatcc taagctcgag tcagagttcg tgtacggtga
     3541 ttacaaggtg tacgatgtga ggaagatgat cgctaagtct gagcaagaga tcggaaaggc
     3601 taccgctaag tatttcttct actctaacat catgaatttc ttcaagaccg agattaccct
     3661 cgctaacggt gagatcagaa agaggccact catcgagaca aacggtgaaa caggtgagat
     3721 cgtgtgggat aagggaaggg atttcgctac cgttagaaag gtgctctcta tgccacaggt
     3781 gaacatcgtt aagaaaaccg aggtgcagac cggtggattc tctaaagagt ctatcctccc
     3841 taagaggaac tctgataagc tcattgctag gaagaaggat tgggacccta agaaatacgg
     3901 tggtttcgat tctcctaccg tggcttactc tgttctcgtt gtggctaagg ttgagaaggg
     3961 aaagagtaag aagctcaagt ctgttaagga acttctcgga atcactatca tggaaaggtc
     4021 atctttcgag aagaacccaa tcgatttcct cgaggctaag ggatacaaag aggttaagaa
     4081 ggatctcatc atcaagctcc caaagtactc actcttcgaa ctcgagaacg gtagaaagag
     4141 gatgctcgct tctgctggtg agcttcaaaa gggaaacgag cttgctctcc catctaagta
     4201 cgttaacttt ctttacctcg cttctcacta cgagaagttg aagggatctc cagaagataa
     4261 cgagcagaag caacttttcg ttgagcagca caagcactac ttggatgaga tcatcgagca
     4321 gatctctgag ttctctaaaa gggtgatcct cgctgatgca aacctcgata aggtgttgtc
     4381 tgcttacaac aagcacagag ataagcctat cagggaacag gcagagaaca tcatccatct
     4441 cttcaccctt accaacctcg gtgctcctgc tgctttcaag tacttcgata caaccatcga
     4501 taggaagaga tacacctcta ccaaagaagt gctcgatgct accctcatcc atcagtctat
     4561 cactggactc tacgagacta ggatcgatct ctcacagctc ggtggtgatt caagggctga
     4621 tcctaagaag aagaggaagg tttgagacgc aggcctccca gctttcgtcc gtatcatcgg
     4681 tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt gcccacacac cagaatccta
     4741 ctaagtttga gtattatggc attggaaaag ctgttttctt ctatcatttg ttctgcttgt
     4801 aatttactgt gttctttcag tttttgtttt cggacatcaa aatgcaaatg gatggataag
     4861 agttaataaa tgatatggtc cttttgttca ttctcaaatt attattatct gttgttttta
     4921 ctttaatggg ttgaatttaa gtaagaaagg aactaacagt gtgatattaa ggtgcaatgt
     4981 tagacatata aaacagtctt tcacctctct ttggttatgt cttgaattgg tttgtttctt
     5041 cacttatctg tgtaatcaag tttactatga gtctatgatc aagtaattat gcaatcaagt
     5101 taagtacagt ataggctttc agcgagaccg gtggtatctt tctagaagat ctcctacaat
     5161 attctcagct gccatggaaa atcgatgttc ttcttttatt ctctcaagat tttcaggctg
     5221 tatattaaaa cttatattaa gaactatgct aaccacctca tcaggaaccg ttgtaggtgg
     5281 cgtgggtttt cttggcaatc gactctcatg aaaactacga gctaaatatt caatatgttc
     5341 ctcttgacca actttattct gcattttttt tgaacgaggt ttagagcaag cttcaggaaa
     5401 ctgagacagg aattttatta aaaatttaaa ttttgaagaa agttcagggt taatagcatc
     5461 cattttttgc tttgcaagtt cctcagcatt cttaacaaaa gacgtctctt ttgacatgtt
     5521 taaagtttaa acctcctgtg tgaaattatt atccgctcat aattccacac attatacgag
     5581 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5641 cgttgcgctc actgccaatt gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5701 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5761 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5821 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5881 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5941 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6001 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6061 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6121 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6181 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6241 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6301 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6361 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6421 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6481 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6541 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6601 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6661 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6721 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6781 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6841 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6901 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6961 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7021 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7081 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7141 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7201 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7261 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7321 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7381 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7441 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7501 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7561 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7621 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7681 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccgc
     7741 ccctgcagcc gaattatatt atttttgcca aataattttt aacaaaagct ctgaagtctt
     7801 cttcatttaa attcttagat gatacttcat ctggaaaatt gtcccaatta gtagcatcac
     7861 gctgtgagta agttctaaac cattttttta ttgttgtatt atctctaatc ttactactcg
     7921 atgagttttc ggtattatct ctatttttaa cttggagcag gttccattca ttgttttttt
     7981 catcatagtg aataaaatca actgctttaa cacttgtgcc tgaacaccat atccatccgg
     8041 cg
//
