LOCUS       sequence-426196-        5630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human ATG16L2 fragment A321 to Q619. N-terminal
            6X His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_ATG16L2:321-619
FEATURES             Location/Qualifiers
     source          1..5630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(655..660,661..677,678..683))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1035..1126
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1051..1068
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1185..1202
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1215..1235
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2270..2404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2307..2326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2361..2380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2433..2598
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2782..3236
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2869..2888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3078..3099
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3262..3366
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3367..3435,3436..4227)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3585..3604)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4398..4986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4887..4906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5140..5157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5291..5515
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(5582..5630,1..485))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
       61 atcacataag caccaagcgc gttggcctca tgcttgagca gattgatgag cgcggtggca
      121 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc
      181 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg
      241 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc
      301 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca
      361 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt
      421 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat
      481 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
      541 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
      601 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
      661 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
      721 cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag
      781 cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc
      841 aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg
      901 cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc
      961 gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct acgtatactc
     1021 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     1081 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     1141 ataccgtccc accatcgggc gcggatctcg gtccgaaaac catgcatcat catcatcatc
     1201 acagcagcgg cagagaaaac ttgtatttcc agggcgctgc ccgacttcct acccgggctc
     1261 aggatgtgct ggatgcccac ctctctgagg tcaatgctgt tcgttttggc cccaacagca
     1321 gcctcctggc cactggaggg gctgaccgcc tgatccacct ctggaatgtt gtgggaagtc
     1381 gcctggaggc caaccagacc ctggagggag ctggtggcag catcaccagt gtggactttg
     1441 acccctcggg ctaccaggtt ttagcagcaa cttacaacca ggctgcccag ctctggaagg
     1501 tgggggaggc acagtccaag gagacactgt ctggacacaa ggataaggtg acagctgcca
     1561 aattcaagct aacgaggcac caggcagtga ctgggagccg cgaccggaca gtgaaggagt
     1621 gggacctcgg ccgtgcctat tgctccagga ccatcaatgt cctttcctac tgtaatgacg
     1681 tggtgtgtgg ggaccatatc atcattagtg gccacaatga ccagaagatc cggttctggg
     1741 acagcagggg gccccactgc acccaggtca tccctgtgca gggccgggtc acctccctga
     1801 gcctcagcca cgaccaactg cacctgctca gctgttcccg agacaacaca ctcaaggtca
     1861 tcgacctgcg tgtcagcaac atccgccagg tgttcagggc cgatggcttc aagtgtggtt
     1921 ctgactggac caaagctgtg ttcagcccgg acagaagcta tgcactggca ggctcctgtg
     1981 atggggccct ttacatctgg gatgtggaca ccgggaaact ggagagcaga ctacagggac
     2041 cccattgcgc tgccgtcaac gccgtggcct ggtgctactc cgggagccac atggtgagcg
     2101 tggaccaggg caggaaggtt gtgctctggc agtgatgacg aagcttgtcg agaagtacta
     2161 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     2221 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2281 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2341 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     2401 gatctgatca ctgatatcgc ctaggagatc cgaaccagat aagtgaaatc tagttccaaa
     2461 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
     2521 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
     2581 ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc
     2641 tgccaactcc atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa
     2701 atttttctgt catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt
     2761 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     2821 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     2881 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     2941 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     3001 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3061 agtccacgtt cttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3121 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3181 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     3241 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3301 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3361 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3421 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3481 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3541 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3601 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3661 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3721 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3781 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3841 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3901 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3961 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4021 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4081 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4141 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4201 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5161 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     5221 atagaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat ggatgccctg
     5281 cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg
     5341 acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt
     5401 gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa
     5461 attgcccgtc gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc
     5521 gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt
     5581 gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
//
