LOCUS       sequence-426200-        6155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human CORO1C. N-terminal 6X His tag
            with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_CORO1C:1-474
FEATURES             Location/Qualifiers
     source          1..6155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    945..1079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(982..1001)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1036..1055
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1108..1273
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1457..1911
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1544..1563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1753..1774
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1937..2041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2042..2110,2111..2902)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2260..2279)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3073..3661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3562..3581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3815..3832
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3966..4190
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4257..4790)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4960..4965,4966..4982,4983..4988))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5340..5431
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5356..5373
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5490..5507
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5520..5540
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 caagagattg ttgctgagaa ggagaaagca catgaaggag caagacccat gagagccatc
       61 ttcctggccg atggcaatgt cttcaccact gggttcagcc gcatgagcga gcggcagctg
      121 gctctctgga atccgaaaaa tatgcaggaa ccaattgctc ttcatgagat ggacactagc
      181 aatggggtgt tgctgccttt ctatgaccct gacaccagca tcatttactt atgtggaaag
      241 ggtgacagca gtattcgcta ttttgagatc acggatgaat ccccgtacgt ccactacctc
      301 aacacattca gcagcaagga gcctcagaga gggatgggtt acatgcccaa gaggggactt
      361 gatgttaaca aatgtgagat tgccagattc ttcaaacttc atgagagaaa gtgtgaacct
      421 attattatga ctgttcccag gaagtctgac cttttccaag atgacctgta tcctgacaca
      481 gcggggccag aggccgcgct ggaggcagaa gagtggttcg aaggcaagaa tgcagaccca
      541 atcctcatct ccttgaagca cgggtacatt ccaggcaaaa acagggatct caaggtggtc
      601 aagaagaaca ttctggatag caagcccact gcaaacaaga agtgcgacct gatcagcatc
      661 cccaagaaaa ccacagacac ggccagtgtg caaaatgaag ccaagttgga tgagatttta
      721 aaagagatca aatctataaa agacacaatc tgcaatcaag atgagcgtat ttccaagtta
      781 gaacagcaga tggcaaagat agcagcctga tgacgaagct tgtcgagaag tactagagga
      841 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
      901 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
      961 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1021 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     1081 gatcactgat atcgcctagg agatccgaac cagataagtg aaatctagtt ccaaactatt
     1141 ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca tctattttgt
     1201 cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt
     1261 ttgtccgccc acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca
     1321 actccatgtg acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt
     1381 tctgtcatct cttcgttatt aatgtttgta attgactgaa tatcaacgct tatttgcagc
     1441 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     1501 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     1561 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     1621 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     1681 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     1741 acgttcttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     1801 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     1861 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     1921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1981 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2041 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     2101 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     2161 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     2221 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     2281 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     2341 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     2401 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     2461 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     2521 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     2581 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     2641 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     2701 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2761 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2821 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2881 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3721 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3781 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3841 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga
     3901 ccagccgcgt aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa
     3961 gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat
     4021 aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa
     4081 agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc
     4141 ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa gactatattc gcggcgttgt
     4201 gacaatttac cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag
     4261 gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta
     4321 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca
     4381 agcgcgttgg cctcatgctt gagcagattg atgagcgcgg tggcaatgcc ctgcctccgg
     4441 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg
     4501 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
     4561 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
     4621 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
     4681 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
     4741 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
     4801 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag
     4861 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     4921 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct
     4981 tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg
     5041 ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc
     5101 tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg
     5161 gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt gccggtggtg
     5221 ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc
     5281 gcccaggact ctagctatag ttctagtggt tggctacgta tactccggaa tattaataga
     5341 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     5401 taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc gtcccaccat
     5461 cgggcgcgga tctcggtccg aaaaccatgc atcatcatca tcatcacagc agcggcagag
     5521 aaaacttgta tttccagggc atgaggcgag tggtacgaca gagcaagttt cggcatgtat
     5581 ttgggcaagc ggtgaaaaat gaccagtgct atgatgacat ccgggtttct cgtgtgacct
     5641 gggatagttc cttttgtgct gtcaatccca gatttgttgc cataatcata gaggcaagtg
     5701 ggggaggagc gttccttgtc ctccctctgc acaagactgg tcgaattgac aaatcttacc
     5761 ctacagtatg tggccacaca ggaccagtgc tggacataga ctggtgccca cataacgatc
     5821 aggtcattgc cagcggttca gaggactgca cggtcatggt atggcagatc ccagaaaatg
     5881 gactcaccct ttccctgact gaacctgtgg tgattttgga aggccactca aagagagtcg
     5941 gcatcgtggc ttggcatcca acggcccgca atgtgcttct tagtgcaggc tgtgataatg
     6001 ccattatcat ctggaatgtg ggaacagggg aagcccttat aaacttggac gatatgcatt
     6061 cagacatgat ttacaatgtg agctggaacc ggaatggcag tctgatctgc acagcttcca
     6121 aagacaagaa agtgagagtc attgatccca ggaaa
//
