LOCUS       sequence-426202-        7508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of full-length human CORO7. N-terminal 6X His tag 
            with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 7508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_CORO7:1-925
FEATURES             Location/Qualifiers
     source          1..7508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1292..1312)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(1325..1342)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(1401..1492)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(1459..1476)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(1844..1849,1850..1866,1867..1872)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2042..2575
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  2642..2866
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(3000..3017)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3171..3759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3251..3270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3930..4721,4722..4790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4553..4572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4791..4895)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4921..5375)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5058..5079)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5269..5288
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     mobile_element  5559..5724
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    5753..5887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5777..5796)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5831..5850
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      6095..6104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gggaagagaa accctcgctt gcgtctgcat cacccacggg tgtctccatc accgcaggct
       61 gggctgtgtc agggaggggc tccgcagggg gcaccagaca ggaagtgaag ctcgggtggg
      121 gccggcaggc ggggttgagg ctgaccttct gcacctgctg gttgtcccca gcccaccagc
      181 tatgggggtc ggtggcaggc acacagccgg cagtgtccgg gaacaggtcc tcgtggaact
      241 ccacagcctt gcggggcaca tggtagccga tgggcacgat ggctgtgtcg ctcagctgta
      301 ggacgcggag tacctcgcag ctcatgacgg ccagcgcctg ccggggcaca agggcagccc
      361 cacgcagcac gctctccagg acacactggg tcactgggct cagcgccggc tgctgcggga
      421 ccacctcgta acagtacagc tgcctctcgc cctttcctgc caggaccagg agcccagagt
      481 cagggtccag cagaggcacg agacacccaa gcgaggtgtc caaggtgagg gaggccaggg
      541 cgctggagaa gaaccgcgtg tcccacagct tcacttcgcg ctcacgcatc tggttgaatc
      601 cagtagacac aaggtgctcc caggtgccca tccatgccag ccggctatcc ctgctgttct
      661 catgggcctg cgtgctctga gaggcccgcg gctttgttct ggggtcaaag atccgcagct
      721 gcttgtcctt gcacgccgtg cccaccaggg ctccatctcg gctccagacg gcgctctgca
      781 ccaggtcccc atgggctgcc agctctgtca ggggctgctg cttggctgcg tcccagacct
      841 tcacagtggt gcctgctgcg ctcaccagaa tgccgtcaga ggtggggtgg aactgcagta
      901 cctccactgg gaggtcctcg gggcccagca ccaccccggg tgctgagggc agggcctggc
      961 caggccctgg cagtcgccag agttttaccg tcctgtcagc cgagcctgtg gccaggagga
     1021 agtcatcaaa gggcgagaag tccaagtcgg tgactaggtc tgaatggcag cccaggtggg
     1081 ccacgcgtcg cttgtcctct ccttggcctt gcagaggcac aatgcccagt acaccaggac
     1141 ggtcggagtt gaaggcgatc aagctgcagc ttgatttgat gtggttcctg catgaagggg
     1201 cggttcctgc tcgaatgtca ctgatccagg actcgcggcg gggcggccga gcctcggtgt
     1261 gccggaactt ggacaccctg aagcggttca tgccctggaa atacaagttt tctctgccgc
     1321 tgctgtgatg atgatgatga tgcatggttt tcggaccgag atccgcgccc gatggtggga
     1381 cggtatgaat aatccggaat atttataggt ttttttatta caaaactgtt acgaaaacag
     1441 taaaatactt atttatttgc gagatggtta tcattttaat tatctccatg atctattaat
     1501 attccggagt atacgtagcc aaccactaga actatagcta gagtcctggg cgaacaaacg
     1561 atgctcgcct tccagaaaac cgaggatgcg aaccacttca tccggggtca gcaccaccgg
     1621 caagcgccgc gacggccgag gtcttccgat ctcctgaagc cagggcagat ccgtgcacag
     1681 caccttgccg tagaagaaca gcaaggccgc caatgcctga cgatgcgtgg agaccgaaac
     1741 cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc
     1801 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagttgacat aagcctgttc
     1861 ggttcgtaaa ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     1921 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     1981 tgtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     2041 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttagg
     2101 tggctcaagt atgggcatca ttcgcacatg taggctcggc cctgaccaag tcaaatccat
     2161 gcgggctgct cttgatcttt tcggtcgtga gttcggagac gtagccacct actcccaaca
     2221 tcagccggac tccgattacc tcgggaactt gctccgtagt aagacattca tcgcgcttgc
     2281 tgccttcgac caagaagcgg ttgttggcgc tctcgcggct tacgttctgc ccaagtttga
     2341 gcagccgcgt agtgagatct atatctatga tctcgcagtc tccggcgagc accggaggca
     2401 gggcattgcc accgcgctca tcaatctgct caagcatgag gccaacgcgc ttggtgctta
     2461 tgtgatctac gtgcaagcag attacggtga cgatcccgca gtggctctct atacaaagtt
     2521 gggcatacgg gaagaagtga tgcactttga tatcgaccca agtaccgcca cctaacaatt
     2581 cgttcaagcc gagatcggct tcccggccgc ggagttgttc ggtaaattgt cacaacgccg
     2641 cgaatatagt ctttaccatg cccttggcca cgcccctctt taatacgacg ggcaatttgc
     2701 acttcagaaa atgaagagtt tgctttagcc ataacaaaag tccagtatgc tttttcacag
     2761 cataactgga ctgatttcag tttacaacta ttctgtctag tttaagactt tattgtcata
     2821 gtttagatct attttgttca gtttaagact ttattgtccg cccacacccg cttacgcagg
     2881 gcatccattt attactcaac cgtaaccgat tttgccaggt tacgcggctg gtctatgcgg
     2941 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     3001 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3061 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3121 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3361 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3601 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3721 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3781 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3841 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4921 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     4981 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     5041 tagggttgag tgttgttcca gtttggaaca agagtccact attaagaacg tggactccaa
     5101 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     5161 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     5221 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     5281 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     5341 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcaaat
     5401 aagcgttgat attcagtcaa ttacaaacat taataacgaa gagatgacag aaaaattttc
     5461 attctgtgac agagaaaaag tagccgaaga tgacggtttg tcacatggag ttggcaggat
     5521 gtttgattaa aaacataaca ggaagaaaaa tgccccgctg tgggcggaca aaatagttgg
     5581 gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt gacaaaatag
     5641 atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca aaatagtttg
     5701 gaactagatt tcacttatct ggttcggatc tcctaggcga tatcagtgat cagatccaga
     5761 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     5821 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     5881 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     5941 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctagt
     6001 acttctcgac aagcttcgtc atcagtccca ctcgtcctcg tccacgcctt caaaggagtc
     6061 ctgggggagt gggtcctccc ggttccccag ttttgccacc atggcattca gcagctcctc
     6121 cttcttttgc tggtcagact tttcttccag gtactgcgct gaggatgggg cccgacgagc
     6181 aggggcctct cggggggctt ggctcactgg gctcatgtca ggaggctgca ggctgagaag
     6241 ccagggctgc ccattagcgc cttgcagcca ggcctcggca ctgagcacag gctcccagat
     6301 cacagccgtg tctgggaaca cgtcatcctg gaagaactct ttccggactc ggggcagccg
     6361 gaaggccaca ggctccaggg aggactgacg cagccgcagg caccgcatca gctccacttc
     6421 ccgcacgtcg cactccgtct taggcaggag gacgaggccc ttgtgggggt caggcgacgt
     6481 gaagctgttg cactccagga agaaagggga ctcggggagc agctcgtaca ggaatacacg
     6541 ggtgtcgccc ttgccggtca ggagcaccag gccagtgtct gggtcgtagc tgggcagcag
     6601 ggttgaggga gccacgtcca ggcccaacac tgccaagggt ccgccggcca gggcctcagc
     6661 ttcatatagg agcagctggc gctcactttg gctgtcaaag ccagacacca gcagacagcg
     6721 accatcacat acccagacaa tgcgagctcc gcgtcctccc ttgggccctg ggccttcctg
     6781 caggggctca gggccactcc ggggcctgta gacccgcaca cgcccatcct tgcagacagt
     6841 ggccagctgc tgcccatcag gactccaggc caggctgaag atctggtctt ggtggccctg
     6901 cagcttcagc cgatcagctc cagcctgaag gtcccagatg cgaacagtga ggtcatagga
     6961 ggacgaggcc agcacattgg ctgccagtgg gtggaagcgc agggagcaga tcttctccgt
     7021 gtggcctgtg agcacagtct ctggcgtggt gagcacctct tccaggccct ctgcgggtac
     7081 ccgccacagt cggatcctgg cgtcctcacc agccacagcg aggcgatggg ggtcaaaggg
     7141 gtcccaggcc agatcagtca cagctgcccc attctgcagc gtgggcagtg ccgtgtcggg
     7201 caggcggcca ggcttccgta gctcaagcac agccacctgt cccccgctgc tgagcagcgg
     7261 cacggccaca cgcagcttgt tggcacagaa gccgtcactc tcaccaggtg tggtgaggtt
     7321 gagccccttg aggttggtga tgtggctgtc tcggtgcagg acagtgccct gagcatggcg
     7381 gaacttggaa ctggggccca gcaggctctg cagcgacctc aaactggggc tggtcccgat
     7441 gccactggtg ctggagagtg agggccccag gctggagggc gtggagggcg aggtcagcga
     7501 actgggag
//
