LOCUS       sequence-426203-        5924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human DMXL1 fragment N2620 to N3016. N-terminal
            6X His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_DMXL1:2620-3016
FEATURES             Location/Qualifiers
     source          1..5924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(220..753)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(923..928,929..945,946..951))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1303..1394
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1319..1336
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1453..1470
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1483..1503
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2832..2966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2869..2888)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2923..2942
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2995..3160
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3344..3798
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3431..3450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3640..3661
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3824..3928
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3929..3997,3998..4789)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4147..4166)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4960..5548
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5449..5468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5702..5719
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  join(5853..5924,1..153)
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 agaatagttg taaactgaaa tcagtccagt tatgctgtga aaaagcatac tggacttttg
       61 ttatggctaa agcaaactct tcattttctg aagtgcaaat tgcccgtcgt attaaagagg
      121 ggcgtggcca agggcatggt aaagactata ttcgcggcgt tgtgacaatt taccgaacaa
      181 ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc
      241 gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg
      301 atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg
      361 cttgagcaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc
      421 gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc
      481 gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg
      541 gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc
      601 gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag
      661 cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc
      721 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
      781 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
      841 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
      901 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
      961 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga
     1021 ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc
     1081 attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc cctggcttca
     1141 ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt
     1201 ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg actctagcta
     1261 tagttctagt ggttggctac gtatactccg gaatattaat agatcatgga gataattaaa
     1321 atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa
     1381 aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc ggatctcggt
     1441 ccgaaaacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
     1501 ggcaatgaga tagaagcaga tttgggatat cctggaggta aagcaagaat tattcataag
     1561 gaatctgata tcattactgc gtttgctgtt aataaggcaa atagaaactg catagcaatc
     1621 gcttccagtc atgatgttca agaactggat gtttctggaa ttctggccac acaggtctac
     1681 acttgggtag atgatgatat agaagtggaa accaaaggat cagaagattt cttggttata
     1741 catgctcgtg atgatttaac agctgttcaa ggtacaactc catatacaca tagcaatcct
     1801 ggcactccaa tcaacatgcc atggcttggt agtacacaga ctggcagagg agcatctgtg
     1861 atgattaaga aagccattaa taatgttaga agaatgactt ctcatccaac tcttccttac
     1921 tatctgacag gagctcaaga tggaagtgtc agaatgtttg aatggggcca ttctcaacaa
     1981 ataacctgtt ttcgatctgg tggcaattca agagttacaa gaatgagatt taactatcag
     2041 ggaaataagt ttggaatagt tgatgctgat ggatatttaa gtttgtatca aacaaactgg
     2101 aaatgttgtc cagttactgg aagcatgcct aagccatacc tgacttggca gtgtcataac
     2161 aaaacagcca atgattttgt cttcgttagt tcctcctctc tgatagctac agctggtctt
     2221 tcaactgaca atagaaacgt atgtttgtgg gatactcttg tagcacctgc caatagttta
     2281 gtccatgcat ttacctgcca tgacagtgga gccacagttt tagcatatgc tccaaaacat
     2341 cagctactaa tatcaggtgg cagaaaaggt tttacatatg tatttgacct ttgtcaacga
     2401 caacagaggc agcttttcca gagccatgat tctcctgtta aagccgttgc tgttgatcca
     2461 actgaagagt actttgttac aggatctgcc gaaggcaata taaagatttg gagtctctct
     2521 acctttggtc ttctccatac ttttgtcagt gaacatgctc ggcagtccat ttttagaaat
     2581 attggaactg gagtgatgca aattgagaca gggcctgcaa atcacatttt ctcctgtgga
     2641 gctgatggaa caatgaaaat gagaatactg ccagatcagt ttagcccttt aaattgatga
     2701 cgaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt
     2761 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     2821 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2881 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2941 tcaatgtatc ttatcatgtc tggatctgat cactgatatc gcctaggaga tccgaaccag
     3001 ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac
     3061 gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat
     3121 ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg
     3181 ttatgttttt aatcaaacat cctgccaact ccatgtgaca aaccgtcatc ttcggctact
     3241 ttttctctgt cacagaatga aaatttttct gtcatctctt cgttattaat gtttgtaatt
     3301 gactgaatat caacgcttat ttgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     3361 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3421 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3481 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3541 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3601 gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag tggactcttg ttccaaactg
     3661 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3721 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3781 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3841 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3901 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3961 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4021 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4081 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4141 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4201 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4261 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4321 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4381 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4441 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4501 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4561 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4621 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4681 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4741 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4801 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4861 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4921 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4981 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5041 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5101 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5161 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5221 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5281 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5341 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5401 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5461 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5521 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5581 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5641 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5701 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     5761 tctgtgcggt atttcacacc gcatagacca gccgcgtaac ctggcaaaat cggttacggt
     5821 tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg
     5881 aacaaaatag atctaaacta tgacaataaa gtcttaaact agac
//
