LOCUS       sequence-426204-        5708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human PAFAH1B1 fragment G86 to R410. N-terminal
            6X His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_PAFAH1B1:86-410
FEATURES             Location/Qualifiers
     source          1..5708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1076..1210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1113..1132)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1167..1186
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1239..1404
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1588..2042
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1675..1694)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1884..1905
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2068..2172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2173..2241,2242..3033)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2391..2410)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3204..3792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3693..3712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3946..3963
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4097..4321
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4388..4921)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5091..5096,5097..5113,5114..5119))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5471..5562
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5487..5504
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5621..5638
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5651..5671
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cgccagaaaa atatgcattg agtggtcaca ggagtccagt cactcgagtc attttccatc
       61 ctgtgttcag tgttatggtc tctgcttcag aggatgctac aattaaggtg tgggattatg
      121 agactggaga ttttgaacga actcttaaag gacatacaga ctctgtacag gacatttcat
      181 tcgaccacag cggcaagctt ctggcttcct gttctgcaga tatgaccatt aaactatggg
      241 attttcaggg ctttgaatgc atcagaacca tgcacggcca tgaccacaat gtttcttcag
      301 tagccatcat gcccaatgga gatcatatag tgtctgcctc aagggataaa actataaaaa
      361 tgtgggaagt gcaaactggc tactgtgtga agacattcac aggacacaga gaatgggtac
      421 gtatggtacg gccaaatcaa gatggcactc tgatagccag ctgttccaat gaccagactg
      481 tgcgtgtatg ggtcgtagca acaaaggaat gcaaggctga gctccgagag catgagcatg
      541 tggtagaatg catttcctgg gctccagaaa gctcatattc ctccatctct gaagcaacag
      601 gatctgagac taaaaaaagt ggtaaacctg ggccattctt gctgtctgga tccagagaca
      661 agactattaa gatgtgggat gtcagtactg gcatgtgcct tatgaccctc gtgggtcatg
      721 ataactgggt acgtggagtt ctgttccatt ctggggggaa gtttattttg agttgtgctg
      781 atgacaagac cctacgcgta tgggattaca agaacaagcg atgcatgaag accctcaatg
      841 cgcatgaaca ctttgttacc tccttggatt tccacaagac ggcaccctat gtcgtcactg
      901 gcagcgtaga tcaaacagta aaagtgtggg agtgccgttg atgacgaagc ttgtcgagaa
      961 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     1021 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     1081 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1141 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     1201 tgtctggatc tgatcactga tatcgcctag gagatccgaa ccagataagt gaaatctagt
     1261 tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca cccagttccc
     1321 atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac ccctcccagt
     1381 tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt ttttaatcaa
     1441 acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct ctgtcacaga
     1501 atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga atatcaacgc
     1561 ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     1621 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1681 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1741 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1801 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1861 cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa cactcaaccc
     1921 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     1981 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     2041 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2101 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2161 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2221 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2281 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2341 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2401 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     2461 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     2521 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     2581 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     2641 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2701 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2761 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2821 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2881 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2941 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3001 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3061 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3121 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3181 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3241 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3301 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3361 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3421 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3481 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3541 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3601 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3661 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3721 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3781 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3841 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3901 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3961 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4021 caccgcatag accagccgcg taacctggca aaatcggtta cggttgagta ataaatggat
     4081 gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa atagatctaa
     4141 actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc agtccagtta
     4201 tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc attttctgaa
     4261 gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatggtaa agactatatt
     4321 cgcggcgttg tgacaattta ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa
     4381 cgaattgtta ggtggcggta cttgggtcga tatcaaagtg catcacttct tcccgtatgc
     4441 ccaactttgt atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca
     4501 cataagcacc aagcgcgttg gcctcatgct tgagcagatt gatgagcgcg gtggcaatgc
     4561 cctgcctccg gtgctcgccg gagactgcga gatcatagat atagatctca ctacgcggct
     4621 gctcaaactt gggcagaacg taagccgcga gagcgccaac aaccgcttct tggtcgaagg
     4681 cagcaagcgc gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct
     4741 gatgttggga gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc
     4801 gcatggattt gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc
     4861 cacctaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca
     4921 tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta
     4981 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
     5041 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa
     5101 ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc
     5161 cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca
     5221 aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg
     5281 tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct
     5341 tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc
     5401 atcgtttgtt cgcccaggac tctagctata gttctagtgg ttggctacgt atactccgga
     5461 atattaatag atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt
     5521 tactgttttc gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac
     5581 cgtcccacca tcgggcgcgg atctcggtcc gaaaaccatg catcatcatc atcatcacag
     5641 cagcggcaga gaaaacttgt atttccaggg cggtcagaaa cgagacccaa aagaatggat
     5701 tccccgtc
//
