LOCUS       sequence-426206-        5765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression of human WDR13 fragment A142 to K485. N-terminal 
            6X His tag with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_WDR13:142-485
FEATURES             Location/Qualifiers
     source          1..5765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..80
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  214..438
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(505..1038)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1208..1213,1214..1230,1231..1236))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1588..1679
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1604..1621
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1738..1755
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1768..1788
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2958..3092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2995..3014)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3049..3068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3121..3286
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3470..3924
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3557..3576)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3766..3787
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3950..4054
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4055..4123,4124..4915)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4273..4292)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5086..5674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5575..5594
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
       61 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
      121 atctgtgcgg tatttcacac cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg
      181 ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact
      241 gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac
      301 tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa
      361 actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc
      421 atggtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa
      481 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
      541 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
      601 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga gcagattgat
      661 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
      721 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
      781 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
      841 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
      901 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
      961 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     1021 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     1081 ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     1141 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
     1201 gcattacagt ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt
     1261 ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg
     1321 gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt caggcattgg cggccttgct
     1381 gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg cttcaggaga tcggaagacc
     1441 tcggccgtcg cggcgcttgc cggtggtgct gaccccggat gaagtggttc gcatcctcgg
     1501 ttttctggaa ggcgagcatc gtttgttcgc ccaggactct agctatagtt ctagtggttg
     1561 gctacgtata ctccggaata ttaatagatc atggagataa ttaaaatgat aaccatctcg
     1621 caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata
     1681 ttccggatta ttcataccgt cccaccatcg ggcgcggatc tcggtccgaa aaccatgcat
     1741 catcatcatc atcacagcag cggcagagaa aacttgtatt tccagggcgc ggaagcgagt
     1801 cgtgcgatgg ctggtgacac gtcgttgtca gaaaactacg cctttgcggg tatgtatcac
     1861 gttttcgacc aacatgtaga tgaggcagtt ccccgtgtga gatttgccaa cgacgacaga
     1921 caccgccttg cgtgttgttc tcttgatggt tcgataagcc tctgccaatt ggttccagct
     1981 cctcccactg tactcagggt actcagaggt catacgagag gtgtctccga ctttgcatgg
     2041 tctctttcta acgatatcct tgtttcaacg tcgctcgatg ctactatgcg tatatgggct
     2101 tcagaggacg gcagatgcat tcgtgagatc cccgacccgg attcagccga gcttctgtgc
     2161 tgcacctttc aacccgtcaa caataatctt acggtcgtag gcaacgccaa acataatgta
     2221 catgttatga acatttcgac aggtaagaaa gttaagggag gctcctctaa gctcactggt
     2281 cgtgtactcg ccctgtcgtt cgacgcccct ggacgtcttc tctgggccgg cgacgaccgc
     2341 ggatcggtgt ttagctttct ctttgatatg gccaccggca aattgaccaa ggctaagaga
     2401 cttgtggttc acgagggttc accagtcacg agtataagcg cgagatcctg ggtctccaga
     2461 gaggcaaggg acccgtcact tctgattaac gcgtgcctga ataaattgct cctttatagg
     2521 gtggtagaca atgagggcac gttgcagttg aagcgttcct ttcctatcga gcagagctca
     2581 catcccgtgc gctcgatctt ttgccccctt atgtcgtttc gtcaaggagc ctgcgtggtg
     2641 acaggttcgg aagatatgtg cgtccatttc ttcgatgttg agcgcgcagc aaaagcggcg
     2701 gtgaataagc tgcaaggcca cagtgctccc gtactggacg tgtcgttcaa ttgcgatgag
     2761 tcgctcttgg cgagttcgga cgccagtggt atggtcatcg tctggaggcg ggagcagaag
     2821 tgatgacgaa gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag
     2881 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     2941 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3061 aactcatcaa tgtatcttat catgtctgga tctgatcact gatatcgcct aggagatccg
     3121 aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct
     3181 tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa
     3241 ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg ggcatttttc
     3301 ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc gtcatcttcg
     3361 gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt attaatgttt
     3421 gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatggga cgcgccctgt
     3481 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3541 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3601 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3661 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     3721 tagacggttt ttcgcccttt gacgttggag tccacgttct taatagtgga ctcttgttcc
     3781 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3841 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3901 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3961 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4021 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4081 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4141 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4201 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4261 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4321 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4381 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4441 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4501 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4561 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4621 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4681 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4741 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4801 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4861 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4921 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4981 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5041 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5101 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5161 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5221 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5281 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5341 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5401 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5461 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5521 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5581 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5641 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5701 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5761 tgtgg
//
